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Fluorescence Modulation of Green Fluorescent Protein Using Fluorinated Unnatural Amino Acids
The ability to modulate protein function through minimal perturbations to amino acid structure represents an ideal mechanism to engineer optimized proteins. Due to the novel spectroscopic properties of green fluorescent protein, it has found widespread application as a reporter protein throughout th...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5806519/ https://www.ncbi.nlm.nih.gov/pubmed/28714902 http://dx.doi.org/10.3390/molecules22071194 |
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author | Villa, Jordan K. Tran, Hong-Anh Vipani, Megha Gianturco, Stephanie Bhasin, Konark Russell, Brent L. Harbron, Elizabeth J. Young, Douglas D. |
author_facet | Villa, Jordan K. Tran, Hong-Anh Vipani, Megha Gianturco, Stephanie Bhasin, Konark Russell, Brent L. Harbron, Elizabeth J. Young, Douglas D. |
author_sort | Villa, Jordan K. |
collection | PubMed |
description | The ability to modulate protein function through minimal perturbations to amino acid structure represents an ideal mechanism to engineer optimized proteins. Due to the novel spectroscopic properties of green fluorescent protein, it has found widespread application as a reporter protein throughout the fields of biology and chemistry. Using site-specific amino acid mutagenesis, we have incorporated various fluorotyrosine residues directly into the fluorophore of the protein, altering the fluorescence and shifting the pKa of the phenolic proton associated with the fluorophore. Relative to wild type GFP, the fluorescence spectrum of the protein is altered with each additional fluorine atom, and the mutant GFPs have the potential to be employed as pH sensors due to the altered electronic properties of the fluorine atoms. |
format | Online Article Text |
id | pubmed-5806519 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-58065192018-02-09 Fluorescence Modulation of Green Fluorescent Protein Using Fluorinated Unnatural Amino Acids Villa, Jordan K. Tran, Hong-Anh Vipani, Megha Gianturco, Stephanie Bhasin, Konark Russell, Brent L. Harbron, Elizabeth J. Young, Douglas D. Molecules Communication The ability to modulate protein function through minimal perturbations to amino acid structure represents an ideal mechanism to engineer optimized proteins. Due to the novel spectroscopic properties of green fluorescent protein, it has found widespread application as a reporter protein throughout the fields of biology and chemistry. Using site-specific amino acid mutagenesis, we have incorporated various fluorotyrosine residues directly into the fluorophore of the protein, altering the fluorescence and shifting the pKa of the phenolic proton associated with the fluorophore. Relative to wild type GFP, the fluorescence spectrum of the protein is altered with each additional fluorine atom, and the mutant GFPs have the potential to be employed as pH sensors due to the altered electronic properties of the fluorine atoms. MDPI 2017-07-16 /pmc/articles/PMC5806519/ /pubmed/28714902 http://dx.doi.org/10.3390/molecules22071194 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Villa, Jordan K. Tran, Hong-Anh Vipani, Megha Gianturco, Stephanie Bhasin, Konark Russell, Brent L. Harbron, Elizabeth J. Young, Douglas D. Fluorescence Modulation of Green Fluorescent Protein Using Fluorinated Unnatural Amino Acids |
title | Fluorescence Modulation of Green Fluorescent Protein Using Fluorinated Unnatural Amino Acids |
title_full | Fluorescence Modulation of Green Fluorescent Protein Using Fluorinated Unnatural Amino Acids |
title_fullStr | Fluorescence Modulation of Green Fluorescent Protein Using Fluorinated Unnatural Amino Acids |
title_full_unstemmed | Fluorescence Modulation of Green Fluorescent Protein Using Fluorinated Unnatural Amino Acids |
title_short | Fluorescence Modulation of Green Fluorescent Protein Using Fluorinated Unnatural Amino Acids |
title_sort | fluorescence modulation of green fluorescent protein using fluorinated unnatural amino acids |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5806519/ https://www.ncbi.nlm.nih.gov/pubmed/28714902 http://dx.doi.org/10.3390/molecules22071194 |
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