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Genome-wide tracking of dCas9-methyltransferase footprints

In normal mammalian development cytosine methylation is essential and is directed to specific regions of the genome. Despite notable advances through mapping its genome-wide distribution, studying the direct contribution of DNA methylation to gene and genome regulation has been limited by the lack o...

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Detalles Bibliográficos
Autores principales: Galonska, Christina, Charlton, Jocelyn, Mattei, Alexandra L., Donaghey, Julie, Clement, Kendell, Gu, Hongcang, Mohammad, Arman W., Stamenova, Elena K., Cacchiarelli, Davide, Klages, Sven, Timmermann, Bernd, Cantz, Tobias, Schöler, Hans R., Gnirke, Andreas, Ziller, Michael J., Meissner, Alexander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5807365/
https://www.ncbi.nlm.nih.gov/pubmed/29426832
http://dx.doi.org/10.1038/s41467-017-02708-5
Descripción
Sumario:In normal mammalian development cytosine methylation is essential and is directed to specific regions of the genome. Despite notable advances through mapping its genome-wide distribution, studying the direct contribution of DNA methylation to gene and genome regulation has been limited by the lack of tools for its precise manipulation. Thus, combining the targeting capability of the CRISPR–Cas9 system with an epigenetic modifier has attracted interest in the scientific community. In contrast to profiling the genome-wide cleavage of a nuclease competent Cas9, tracing the global activity of a dead Cas9 (dCas9) methyltransferase fusion protein is challenging within a highly methylated genome. Here, we report the generation and use of an engineered, methylation depleted but maintenance competent mouse ES cell line and find surprisingly ubiquitous nuclear activity of dCas9-methyltransferases. Subsequent experiments in human somatic cells refine these observations and point to an important difference between genetic and epigenetic editing tools that require unique experimental considerations.