Isolation and complete genome analysis of neurotropic dengue virus serotype 3 from the cerebrospinal fluid of an encephalitis patient

Although neurological manifestations associated with dengue viruses (DENV) infection have been reported, there is very limited information on the genetic characteristics of neurotropic DENV. Here we describe the isolation and complete genome analysis of DENV serotype 3 (DENV-3) from cerebrospinal fl...

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Autores principales: Dhenni, Rama, Karyanti, Mulya Rahma, Putri, Nina Dwi, Yohan, Benediktus, Yudhaputri, Frilasita A., Ma'roef, Chairin Nisa, Fadhilah, Araniy, Perkasa, Aditya, Restuadi, Restuadi, Trimarsanto, Hidayat, Mangunatmadja, Irawan, Ledermann, Jeremy P., Rosenberg, Ronald, Powers, Ann M., Myint, Khin Saw Aye, Sasmono, R. Tedjo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5809095/
https://www.ncbi.nlm.nih.gov/pubmed/29329287
http://dx.doi.org/10.1371/journal.pntd.0006198
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author Dhenni, Rama
Karyanti, Mulya Rahma
Putri, Nina Dwi
Yohan, Benediktus
Yudhaputri, Frilasita A.
Ma'roef, Chairin Nisa
Fadhilah, Araniy
Perkasa, Aditya
Restuadi, Restuadi
Trimarsanto, Hidayat
Mangunatmadja, Irawan
Ledermann, Jeremy P.
Rosenberg, Ronald
Powers, Ann M.
Myint, Khin Saw Aye
Sasmono, R. Tedjo
author_facet Dhenni, Rama
Karyanti, Mulya Rahma
Putri, Nina Dwi
Yohan, Benediktus
Yudhaputri, Frilasita A.
Ma'roef, Chairin Nisa
Fadhilah, Araniy
Perkasa, Aditya
Restuadi, Restuadi
Trimarsanto, Hidayat
Mangunatmadja, Irawan
Ledermann, Jeremy P.
Rosenberg, Ronald
Powers, Ann M.
Myint, Khin Saw Aye
Sasmono, R. Tedjo
author_sort Dhenni, Rama
collection PubMed
description Although neurological manifestations associated with dengue viruses (DENV) infection have been reported, there is very limited information on the genetic characteristics of neurotropic DENV. Here we describe the isolation and complete genome analysis of DENV serotype 3 (DENV-3) from cerebrospinal fluid of an encephalitis paediatric patient in Jakarta, Indonesia. Next-generation sequencing was employed to deduce the complete genome of the neurotropic DENV-3 isolate. Based on complete genome analysis, two unique and nine uncommon amino acid changes in the protein coding region were observed in the virus. A phylogenetic tree and molecular clock analysis revealed that the neurotropic virus was a member of Sumatran-Javan clade of DENV-3 genotype I and shared a common ancestor with other isolates from Jakarta around 1998. This is the first report of neurotropic DENV-3 complete genome analysis, providing detailed information on the genetic characteristics of this virus.
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spelling pubmed-58090952018-02-28 Isolation and complete genome analysis of neurotropic dengue virus serotype 3 from the cerebrospinal fluid of an encephalitis patient Dhenni, Rama Karyanti, Mulya Rahma Putri, Nina Dwi Yohan, Benediktus Yudhaputri, Frilasita A. Ma'roef, Chairin Nisa Fadhilah, Araniy Perkasa, Aditya Restuadi, Restuadi Trimarsanto, Hidayat Mangunatmadja, Irawan Ledermann, Jeremy P. Rosenberg, Ronald Powers, Ann M. Myint, Khin Saw Aye Sasmono, R. Tedjo PLoS Negl Trop Dis Research Article Although neurological manifestations associated with dengue viruses (DENV) infection have been reported, there is very limited information on the genetic characteristics of neurotropic DENV. Here we describe the isolation and complete genome analysis of DENV serotype 3 (DENV-3) from cerebrospinal fluid of an encephalitis paediatric patient in Jakarta, Indonesia. Next-generation sequencing was employed to deduce the complete genome of the neurotropic DENV-3 isolate. Based on complete genome analysis, two unique and nine uncommon amino acid changes in the protein coding region were observed in the virus. A phylogenetic tree and molecular clock analysis revealed that the neurotropic virus was a member of Sumatran-Javan clade of DENV-3 genotype I and shared a common ancestor with other isolates from Jakarta around 1998. This is the first report of neurotropic DENV-3 complete genome analysis, providing detailed information on the genetic characteristics of this virus. Public Library of Science 2018-01-12 /pmc/articles/PMC5809095/ /pubmed/29329287 http://dx.doi.org/10.1371/journal.pntd.0006198 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Dhenni, Rama
Karyanti, Mulya Rahma
Putri, Nina Dwi
Yohan, Benediktus
Yudhaputri, Frilasita A.
Ma'roef, Chairin Nisa
Fadhilah, Araniy
Perkasa, Aditya
Restuadi, Restuadi
Trimarsanto, Hidayat
Mangunatmadja, Irawan
Ledermann, Jeremy P.
Rosenberg, Ronald
Powers, Ann M.
Myint, Khin Saw Aye
Sasmono, R. Tedjo
Isolation and complete genome analysis of neurotropic dengue virus serotype 3 from the cerebrospinal fluid of an encephalitis patient
title Isolation and complete genome analysis of neurotropic dengue virus serotype 3 from the cerebrospinal fluid of an encephalitis patient
title_full Isolation and complete genome analysis of neurotropic dengue virus serotype 3 from the cerebrospinal fluid of an encephalitis patient
title_fullStr Isolation and complete genome analysis of neurotropic dengue virus serotype 3 from the cerebrospinal fluid of an encephalitis patient
title_full_unstemmed Isolation and complete genome analysis of neurotropic dengue virus serotype 3 from the cerebrospinal fluid of an encephalitis patient
title_short Isolation and complete genome analysis of neurotropic dengue virus serotype 3 from the cerebrospinal fluid of an encephalitis patient
title_sort isolation and complete genome analysis of neurotropic dengue virus serotype 3 from the cerebrospinal fluid of an encephalitis patient
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5809095/
https://www.ncbi.nlm.nih.gov/pubmed/29329287
http://dx.doi.org/10.1371/journal.pntd.0006198
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