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Expression and replication of virus-like circular DNA in human cells
The consumption of bovine milk and meat is considered a risk factor for colon- and breast cancer formation, and milk consumption has also been implicated in an increased risk for developing Multiple Sclerosis (MS). A number of highly related virus-like DNAs have been recently isolated from bovine mi...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5809378/ https://www.ncbi.nlm.nih.gov/pubmed/29434270 http://dx.doi.org/10.1038/s41598-018-21317-w |
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author | Eilebrecht, Sebastian Hotz-Wagenblatt, Agnes Sarachaga, Victor Burk, Amelie Falida, Konstantina Chakraborty, Deblina Nikitina, Ekaterina Tessmer, Claudia Whitley, Corinna Sauerland, Charlotte Gunst, Karin Grewe, Imke Bund, Timo |
author_facet | Eilebrecht, Sebastian Hotz-Wagenblatt, Agnes Sarachaga, Victor Burk, Amelie Falida, Konstantina Chakraborty, Deblina Nikitina, Ekaterina Tessmer, Claudia Whitley, Corinna Sauerland, Charlotte Gunst, Karin Grewe, Imke Bund, Timo |
author_sort | Eilebrecht, Sebastian |
collection | PubMed |
description | The consumption of bovine milk and meat is considered a risk factor for colon- and breast cancer formation, and milk consumption has also been implicated in an increased risk for developing Multiple Sclerosis (MS). A number of highly related virus-like DNAs have been recently isolated from bovine milk and sera and from a brain sample of a MS patient. As a genetic activity of these Acinetobacter-related bovine milk and meat factors (BMMFs) is unknown in eukaryotes, we analyzed their expression and replication potential in human HEK293TT cells. While all analyzed BMMFs show transcriptional activity, the MS brain isolate MSBI1.176, sharing homology with a transmissible spongiform encephalopathy-associated DNA molecule, is transcribed at highest levels. We show expression of a replication-associated protein (Rep), which is highly conserved among all BMMFs, and serological tests indicate a human anti-Rep immune response. While the cow milk isolate CMI1.252 is replication-competent in HEK293TT cells, replication of MSBI1.176 is complemented by CMI1.252, pointing at an interplay during the establishment of persistence in human cells. Transcriptome profiling upon BMMF expression identified host cellular gene expression changes related to cell cycle progression and cell viability control, indicating potential pathways for a pathogenic involvement of BMMFs. |
format | Online Article Text |
id | pubmed-5809378 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-58093782018-02-15 Expression and replication of virus-like circular DNA in human cells Eilebrecht, Sebastian Hotz-Wagenblatt, Agnes Sarachaga, Victor Burk, Amelie Falida, Konstantina Chakraborty, Deblina Nikitina, Ekaterina Tessmer, Claudia Whitley, Corinna Sauerland, Charlotte Gunst, Karin Grewe, Imke Bund, Timo Sci Rep Article The consumption of bovine milk and meat is considered a risk factor for colon- and breast cancer formation, and milk consumption has also been implicated in an increased risk for developing Multiple Sclerosis (MS). A number of highly related virus-like DNAs have been recently isolated from bovine milk and sera and from a brain sample of a MS patient. As a genetic activity of these Acinetobacter-related bovine milk and meat factors (BMMFs) is unknown in eukaryotes, we analyzed their expression and replication potential in human HEK293TT cells. While all analyzed BMMFs show transcriptional activity, the MS brain isolate MSBI1.176, sharing homology with a transmissible spongiform encephalopathy-associated DNA molecule, is transcribed at highest levels. We show expression of a replication-associated protein (Rep), which is highly conserved among all BMMFs, and serological tests indicate a human anti-Rep immune response. While the cow milk isolate CMI1.252 is replication-competent in HEK293TT cells, replication of MSBI1.176 is complemented by CMI1.252, pointing at an interplay during the establishment of persistence in human cells. Transcriptome profiling upon BMMF expression identified host cellular gene expression changes related to cell cycle progression and cell viability control, indicating potential pathways for a pathogenic involvement of BMMFs. Nature Publishing Group UK 2018-02-12 /pmc/articles/PMC5809378/ /pubmed/29434270 http://dx.doi.org/10.1038/s41598-018-21317-w Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Eilebrecht, Sebastian Hotz-Wagenblatt, Agnes Sarachaga, Victor Burk, Amelie Falida, Konstantina Chakraborty, Deblina Nikitina, Ekaterina Tessmer, Claudia Whitley, Corinna Sauerland, Charlotte Gunst, Karin Grewe, Imke Bund, Timo Expression and replication of virus-like circular DNA in human cells |
title | Expression and replication of virus-like circular DNA in human cells |
title_full | Expression and replication of virus-like circular DNA in human cells |
title_fullStr | Expression and replication of virus-like circular DNA in human cells |
title_full_unstemmed | Expression and replication of virus-like circular DNA in human cells |
title_short | Expression and replication of virus-like circular DNA in human cells |
title_sort | expression and replication of virus-like circular dna in human cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5809378/ https://www.ncbi.nlm.nih.gov/pubmed/29434270 http://dx.doi.org/10.1038/s41598-018-21317-w |
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