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Nemopilema nomurai jellyfish venom exerts an anti-metastatic effect by inhibiting Smad- and NF-κB-mediated epithelial–mesenchymal transition in HepG2 cells

Epithelial–mesenchymal transition (EMT) is a key initial step in metastasis for malignant cancer cells to obtain invasive and motile properties. Inhibiting EMT has become a new strategy for cancer therapy. In our previous in vivo study, Nemopilema nomurai jellyfish venom (NnV) -treated HepG2 xenogra...

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Autores principales: Lee, Hyunkyoung, Pyo, Min Jung, Bae, Seong Kyeong, Heo, Yunwi, Choudhary, Indu, Hwang, Duhyeon, Yang, Hyeryeon, Kim, Je-hein, Chae, Jinho, Han, Chang Hoon, Kang, Changkeun, Yum, Seungshic, Kim, Euikyung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5809415/
https://www.ncbi.nlm.nih.gov/pubmed/29434219
http://dx.doi.org/10.1038/s41598-018-20724-3
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author Lee, Hyunkyoung
Pyo, Min Jung
Bae, Seong Kyeong
Heo, Yunwi
Choudhary, Indu
Hwang, Duhyeon
Yang, Hyeryeon
Kim, Je-hein
Chae, Jinho
Han, Chang Hoon
Kang, Changkeun
Yum, Seungshic
Kim, Euikyung
author_facet Lee, Hyunkyoung
Pyo, Min Jung
Bae, Seong Kyeong
Heo, Yunwi
Choudhary, Indu
Hwang, Duhyeon
Yang, Hyeryeon
Kim, Je-hein
Chae, Jinho
Han, Chang Hoon
Kang, Changkeun
Yum, Seungshic
Kim, Euikyung
author_sort Lee, Hyunkyoung
collection PubMed
description Epithelial–mesenchymal transition (EMT) is a key initial step in metastasis for malignant cancer cells to obtain invasive and motile properties. Inhibiting EMT has become a new strategy for cancer therapy. In our previous in vivo study, Nemopilema nomurai jellyfish venom (NnV) -treated HepG2 xenograft mice group showed that E-cadherin expression was strongly detected compared with non-treated groups. Therefore, this study aimed to determine whether NnV could inhibit the invasive and migratory abilities of HepG2 human hepatocellular carcinoma cells and to examine its effect on EMT. Our results revealed that transforming growth factor (TGF)-β1 induced cell morphological changes and downregulated E-cadherin and β-catenin expression, but upregulated N-cadherin and vimentin expression through the Smad and NF-κB pathways in HepG2 cells. Treatment of TGF-β1-stimulated HepG2 cells with NnV reversed the EMT-related marker expression, thereby inhibiting cell migration and invasion. NnV also significantly suppressed the activation of p-Smad3, Smad4, and p-NF-κB in a dose-dependent manner. These data indicated that NnV can significantly suppress cell migration and invasion by inhibiting EMT in HepG2 cells, and therefore might be a promising target for hepatocellular carcinoma therapeutics.
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spelling pubmed-58094152018-02-15 Nemopilema nomurai jellyfish venom exerts an anti-metastatic effect by inhibiting Smad- and NF-κB-mediated epithelial–mesenchymal transition in HepG2 cells Lee, Hyunkyoung Pyo, Min Jung Bae, Seong Kyeong Heo, Yunwi Choudhary, Indu Hwang, Duhyeon Yang, Hyeryeon Kim, Je-hein Chae, Jinho Han, Chang Hoon Kang, Changkeun Yum, Seungshic Kim, Euikyung Sci Rep Article Epithelial–mesenchymal transition (EMT) is a key initial step in metastasis for malignant cancer cells to obtain invasive and motile properties. Inhibiting EMT has become a new strategy for cancer therapy. In our previous in vivo study, Nemopilema nomurai jellyfish venom (NnV) -treated HepG2 xenograft mice group showed that E-cadherin expression was strongly detected compared with non-treated groups. Therefore, this study aimed to determine whether NnV could inhibit the invasive and migratory abilities of HepG2 human hepatocellular carcinoma cells and to examine its effect on EMT. Our results revealed that transforming growth factor (TGF)-β1 induced cell morphological changes and downregulated E-cadherin and β-catenin expression, but upregulated N-cadherin and vimentin expression through the Smad and NF-κB pathways in HepG2 cells. Treatment of TGF-β1-stimulated HepG2 cells with NnV reversed the EMT-related marker expression, thereby inhibiting cell migration and invasion. NnV also significantly suppressed the activation of p-Smad3, Smad4, and p-NF-κB in a dose-dependent manner. These data indicated that NnV can significantly suppress cell migration and invasion by inhibiting EMT in HepG2 cells, and therefore might be a promising target for hepatocellular carcinoma therapeutics. Nature Publishing Group UK 2018-02-12 /pmc/articles/PMC5809415/ /pubmed/29434219 http://dx.doi.org/10.1038/s41598-018-20724-3 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Lee, Hyunkyoung
Pyo, Min Jung
Bae, Seong Kyeong
Heo, Yunwi
Choudhary, Indu
Hwang, Duhyeon
Yang, Hyeryeon
Kim, Je-hein
Chae, Jinho
Han, Chang Hoon
Kang, Changkeun
Yum, Seungshic
Kim, Euikyung
Nemopilema nomurai jellyfish venom exerts an anti-metastatic effect by inhibiting Smad- and NF-κB-mediated epithelial–mesenchymal transition in HepG2 cells
title Nemopilema nomurai jellyfish venom exerts an anti-metastatic effect by inhibiting Smad- and NF-κB-mediated epithelial–mesenchymal transition in HepG2 cells
title_full Nemopilema nomurai jellyfish venom exerts an anti-metastatic effect by inhibiting Smad- and NF-κB-mediated epithelial–mesenchymal transition in HepG2 cells
title_fullStr Nemopilema nomurai jellyfish venom exerts an anti-metastatic effect by inhibiting Smad- and NF-κB-mediated epithelial–mesenchymal transition in HepG2 cells
title_full_unstemmed Nemopilema nomurai jellyfish venom exerts an anti-metastatic effect by inhibiting Smad- and NF-κB-mediated epithelial–mesenchymal transition in HepG2 cells
title_short Nemopilema nomurai jellyfish venom exerts an anti-metastatic effect by inhibiting Smad- and NF-κB-mediated epithelial–mesenchymal transition in HepG2 cells
title_sort nemopilema nomurai jellyfish venom exerts an anti-metastatic effect by inhibiting smad- and nf-κb-mediated epithelial–mesenchymal transition in hepg2 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5809415/
https://www.ncbi.nlm.nih.gov/pubmed/29434219
http://dx.doi.org/10.1038/s41598-018-20724-3
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