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Retinal progenitor cells release extracellular vesicles containing developmental transcription factors, microRNA and membrane proteins

A range of cell types, including embryonic stem cells, neurons and astrocytes have been shown to release extracellular vesicles (EVs) containing molecular cargo. Across cell types, EVs facilitate transfer of mRNA, microRNA and proteins between cells. Here we describe the release kinetics and content...

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Autores principales: Zhou, Jing, Benito-Martin, Alberto, Mighty, Jason, Chang, Lynne, Ghoroghi, Shima, Wu, Hao, Wong, Madeline, Guariglia, Sara, Baranov, Petr, Young, Michael, Gharbaran, Rajendra, Emerson, Mark, Mark, Milica Tesic, Molina, Henrik, Canto-Soler, M. Valeria, Selgas, Hector Peinado, Redenti, Stephen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5809580/
https://www.ncbi.nlm.nih.gov/pubmed/29434302
http://dx.doi.org/10.1038/s41598-018-20421-1
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author Zhou, Jing
Benito-Martin, Alberto
Mighty, Jason
Chang, Lynne
Ghoroghi, Shima
Wu, Hao
Wong, Madeline
Guariglia, Sara
Baranov, Petr
Young, Michael
Gharbaran, Rajendra
Emerson, Mark
Mark, Milica Tesic
Molina, Henrik
Canto-Soler, M. Valeria
Selgas, Hector Peinado
Redenti, Stephen
author_facet Zhou, Jing
Benito-Martin, Alberto
Mighty, Jason
Chang, Lynne
Ghoroghi, Shima
Wu, Hao
Wong, Madeline
Guariglia, Sara
Baranov, Petr
Young, Michael
Gharbaran, Rajendra
Emerson, Mark
Mark, Milica Tesic
Molina, Henrik
Canto-Soler, M. Valeria
Selgas, Hector Peinado
Redenti, Stephen
author_sort Zhou, Jing
collection PubMed
description A range of cell types, including embryonic stem cells, neurons and astrocytes have been shown to release extracellular vesicles (EVs) containing molecular cargo. Across cell types, EVs facilitate transfer of mRNA, microRNA and proteins between cells. Here we describe the release kinetics and content of EVs from mouse retinal progenitor cells (mRPCs). Interestingly, mRPC derived EVs contain mRNA, miRNA and proteins associated with multipotency and retinal development. Transcripts enclosed in mRPC EVs, include the transcription factors Pax6, Hes1, and Sox2, a mitotic chromosome stabilizer Ki67, and the neural intermediate filaments Nestin and GFAP. Proteomic analysis of EV content revealed retinogenic growth factors and morphogen proteins. mRPC EVs were shown to transfer GFP mRNA between cell populations. Finally, analysis of EV mediated functional cargo delivery, using the Cre-loxP recombination system, revealed transfer and uptake of Cre+ EVs, which were then internalized by target mRPCs activating responder loxP GFP expression. In summary, the data supports a paradigm of EV genetic material encapsulation and transfer within RPC populations. RPC EV transfer may influence recipient RPC transcriptional and post-transcriptional regulation, representing a novel mechanism of differentiation and fate determination during retinal development.
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spelling pubmed-58095802018-02-15 Retinal progenitor cells release extracellular vesicles containing developmental transcription factors, microRNA and membrane proteins Zhou, Jing Benito-Martin, Alberto Mighty, Jason Chang, Lynne Ghoroghi, Shima Wu, Hao Wong, Madeline Guariglia, Sara Baranov, Petr Young, Michael Gharbaran, Rajendra Emerson, Mark Mark, Milica Tesic Molina, Henrik Canto-Soler, M. Valeria Selgas, Hector Peinado Redenti, Stephen Sci Rep Article A range of cell types, including embryonic stem cells, neurons and astrocytes have been shown to release extracellular vesicles (EVs) containing molecular cargo. Across cell types, EVs facilitate transfer of mRNA, microRNA and proteins between cells. Here we describe the release kinetics and content of EVs from mouse retinal progenitor cells (mRPCs). Interestingly, mRPC derived EVs contain mRNA, miRNA and proteins associated with multipotency and retinal development. Transcripts enclosed in mRPC EVs, include the transcription factors Pax6, Hes1, and Sox2, a mitotic chromosome stabilizer Ki67, and the neural intermediate filaments Nestin and GFAP. Proteomic analysis of EV content revealed retinogenic growth factors and morphogen proteins. mRPC EVs were shown to transfer GFP mRNA between cell populations. Finally, analysis of EV mediated functional cargo delivery, using the Cre-loxP recombination system, revealed transfer and uptake of Cre+ EVs, which were then internalized by target mRPCs activating responder loxP GFP expression. In summary, the data supports a paradigm of EV genetic material encapsulation and transfer within RPC populations. RPC EV transfer may influence recipient RPC transcriptional and post-transcriptional regulation, representing a novel mechanism of differentiation and fate determination during retinal development. Nature Publishing Group UK 2018-02-12 /pmc/articles/PMC5809580/ /pubmed/29434302 http://dx.doi.org/10.1038/s41598-018-20421-1 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Zhou, Jing
Benito-Martin, Alberto
Mighty, Jason
Chang, Lynne
Ghoroghi, Shima
Wu, Hao
Wong, Madeline
Guariglia, Sara
Baranov, Petr
Young, Michael
Gharbaran, Rajendra
Emerson, Mark
Mark, Milica Tesic
Molina, Henrik
Canto-Soler, M. Valeria
Selgas, Hector Peinado
Redenti, Stephen
Retinal progenitor cells release extracellular vesicles containing developmental transcription factors, microRNA and membrane proteins
title Retinal progenitor cells release extracellular vesicles containing developmental transcription factors, microRNA and membrane proteins
title_full Retinal progenitor cells release extracellular vesicles containing developmental transcription factors, microRNA and membrane proteins
title_fullStr Retinal progenitor cells release extracellular vesicles containing developmental transcription factors, microRNA and membrane proteins
title_full_unstemmed Retinal progenitor cells release extracellular vesicles containing developmental transcription factors, microRNA and membrane proteins
title_short Retinal progenitor cells release extracellular vesicles containing developmental transcription factors, microRNA and membrane proteins
title_sort retinal progenitor cells release extracellular vesicles containing developmental transcription factors, microrna and membrane proteins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5809580/
https://www.ncbi.nlm.nih.gov/pubmed/29434302
http://dx.doi.org/10.1038/s41598-018-20421-1
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