Rapid one-step biotinylation of biological and non-biological surfaces

We describe a rapid one-step method to biotinylate virtually any biological or non-biological surface. Contacting a solution of biotin-spacer-lipid constructs with a surface will form a coating within seconds on non-biological surfaces or within minutes on most biological membranes including membran...

Descripción completa

Detalles Bibliográficos
Autores principales: Henry, Stephen, Williams, Eleanor, Barr, Katie, Korchagina, Elena, Tuzikov, Alexandr, Ilyushina, Natalia, Abayzeed, Sidahmed A., Webb, Kevin F., Bovin, Nicolai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5809608/
https://www.ncbi.nlm.nih.gov/pubmed/29434224
http://dx.doi.org/10.1038/s41598-018-21186-3
_version_ 1783299596681740288
author Henry, Stephen
Williams, Eleanor
Barr, Katie
Korchagina, Elena
Tuzikov, Alexandr
Ilyushina, Natalia
Abayzeed, Sidahmed A.
Webb, Kevin F.
Bovin, Nicolai
author_facet Henry, Stephen
Williams, Eleanor
Barr, Katie
Korchagina, Elena
Tuzikov, Alexandr
Ilyushina, Natalia
Abayzeed, Sidahmed A.
Webb, Kevin F.
Bovin, Nicolai
author_sort Henry, Stephen
collection PubMed
description We describe a rapid one-step method to biotinylate virtually any biological or non-biological surface. Contacting a solution of biotin-spacer-lipid constructs with a surface will form a coating within seconds on non-biological surfaces or within minutes on most biological membranes including membrane viruses. The resultant biotinylated surface can then be used to interact with avidinylated conjugates, beads, vesicles, surfaces or cells.
format Online
Article
Text
id pubmed-5809608
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-58096082018-02-15 Rapid one-step biotinylation of biological and non-biological surfaces Henry, Stephen Williams, Eleanor Barr, Katie Korchagina, Elena Tuzikov, Alexandr Ilyushina, Natalia Abayzeed, Sidahmed A. Webb, Kevin F. Bovin, Nicolai Sci Rep Article We describe a rapid one-step method to biotinylate virtually any biological or non-biological surface. Contacting a solution of biotin-spacer-lipid constructs with a surface will form a coating within seconds on non-biological surfaces or within minutes on most biological membranes including membrane viruses. The resultant biotinylated surface can then be used to interact with avidinylated conjugates, beads, vesicles, surfaces or cells. Nature Publishing Group UK 2018-02-12 /pmc/articles/PMC5809608/ /pubmed/29434224 http://dx.doi.org/10.1038/s41598-018-21186-3 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Henry, Stephen
Williams, Eleanor
Barr, Katie
Korchagina, Elena
Tuzikov, Alexandr
Ilyushina, Natalia
Abayzeed, Sidahmed A.
Webb, Kevin F.
Bovin, Nicolai
Rapid one-step biotinylation of biological and non-biological surfaces
title Rapid one-step biotinylation of biological and non-biological surfaces
title_full Rapid one-step biotinylation of biological and non-biological surfaces
title_fullStr Rapid one-step biotinylation of biological and non-biological surfaces
title_full_unstemmed Rapid one-step biotinylation of biological and non-biological surfaces
title_short Rapid one-step biotinylation of biological and non-biological surfaces
title_sort rapid one-step biotinylation of biological and non-biological surfaces
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5809608/
https://www.ncbi.nlm.nih.gov/pubmed/29434224
http://dx.doi.org/10.1038/s41598-018-21186-3
work_keys_str_mv AT henrystephen rapidonestepbiotinylationofbiologicalandnonbiologicalsurfaces
AT williamseleanor rapidonestepbiotinylationofbiologicalandnonbiologicalsurfaces
AT barrkatie rapidonestepbiotinylationofbiologicalandnonbiologicalsurfaces
AT korchaginaelena rapidonestepbiotinylationofbiologicalandnonbiologicalsurfaces
AT tuzikovalexandr rapidonestepbiotinylationofbiologicalandnonbiologicalsurfaces
AT ilyushinanatalia rapidonestepbiotinylationofbiologicalandnonbiologicalsurfaces
AT abayzeedsidahmeda rapidonestepbiotinylationofbiologicalandnonbiologicalsurfaces
AT webbkevinf rapidonestepbiotinylationofbiologicalandnonbiologicalsurfaces
AT bovinnicolai rapidonestepbiotinylationofbiologicalandnonbiologicalsurfaces

Ejemplares similares