The Cellular Chaperone Heat Shock Protein 90 Is Required for Foot-and-Mouth Disease Virus Capsid Precursor Processing and Assembly of Capsid Pentamers
Productive picornavirus infection requires the hijacking of host cell pathways to aid with the different stages of virus entry, synthesis of the viral polyprotein, and viral genome replication. Many picornaviruses, including foot-and-mouth disease virus (FMDV), assemble capsids via the multimerizati...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5809743/ https://www.ncbi.nlm.nih.gov/pubmed/29212943 http://dx.doi.org/10.1128/JVI.01415-17 |
_version_ | 1783299610660306944 |
---|---|
author | Newman, Joseph Asfor, Amin S. Berryman, Stephen Jackson, Terry Curry, Stephen Tuthill, Tobias J. |
author_facet | Newman, Joseph Asfor, Amin S. Berryman, Stephen Jackson, Terry Curry, Stephen Tuthill, Tobias J. |
author_sort | Newman, Joseph |
collection | PubMed |
description | Productive picornavirus infection requires the hijacking of host cell pathways to aid with the different stages of virus entry, synthesis of the viral polyprotein, and viral genome replication. Many picornaviruses, including foot-and-mouth disease virus (FMDV), assemble capsids via the multimerization of several copies of a single capsid precursor protein into a pentameric subunit which further encapsidates the RNA. Pentamer formation is preceded by co- and posttranslational modification of the capsid precursor (P1-2A) by viral and cellular enzymes and the subsequent rearrangement of P1-2A into a structure amenable to pentamer formation. We have developed a cell-free system to study FMDV pentamer assembly using recombinantly expressed FMDV capsid precursor and 3C protease. Using this assay, we have shown that two structurally different inhibitors of the cellular chaperone heat shock protein 90 (hsp90) impeded FMDV capsid precursor processing and subsequent pentamer formation. Treatment of FMDV permissive cells with the hsp90 inhibitor prior to infection reduced the endpoint titer by more than 10-fold while not affecting the activity of a subgenomic replicon, indicating that translation and replication of viral RNA were unaffected by the drug. IMPORTANCE FMDV of the Picornaviridae family is a pathogen of huge economic importance to the livestock industry due to its effect on the restriction of livestock movement and necessary control measures required following an outbreak. The study of FMDV capsid assembly, and picornavirus capsid assembly more generally, has tended to be focused upon the formation of capsids from pentameric intermediates or the immediate cotranslational modification of the capsid precursor protein. Here, we describe a system to analyze the early stages of FMDV pentameric capsid intermediate assembly and demonstrate a novel requirement for the cellular chaperone hsp90 in the formation of these pentameric intermediates. We show the added complexity involved for this process to occur, which could be the basis for a novel antiviral control mechanism for FMDV. |
format | Online Article Text |
id | pubmed-5809743 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-58097432018-02-21 The Cellular Chaperone Heat Shock Protein 90 Is Required for Foot-and-Mouth Disease Virus Capsid Precursor Processing and Assembly of Capsid Pentamers Newman, Joseph Asfor, Amin S. Berryman, Stephen Jackson, Terry Curry, Stephen Tuthill, Tobias J. J Virol Structure and Assembly Productive picornavirus infection requires the hijacking of host cell pathways to aid with the different stages of virus entry, synthesis of the viral polyprotein, and viral genome replication. Many picornaviruses, including foot-and-mouth disease virus (FMDV), assemble capsids via the multimerization of several copies of a single capsid precursor protein into a pentameric subunit which further encapsidates the RNA. Pentamer formation is preceded by co- and posttranslational modification of the capsid precursor (P1-2A) by viral and cellular enzymes and the subsequent rearrangement of P1-2A into a structure amenable to pentamer formation. We have developed a cell-free system to study FMDV pentamer assembly using recombinantly expressed FMDV capsid precursor and 3C protease. Using this assay, we have shown that two structurally different inhibitors of the cellular chaperone heat shock protein 90 (hsp90) impeded FMDV capsid precursor processing and subsequent pentamer formation. Treatment of FMDV permissive cells with the hsp90 inhibitor prior to infection reduced the endpoint titer by more than 10-fold while not affecting the activity of a subgenomic replicon, indicating that translation and replication of viral RNA were unaffected by the drug. IMPORTANCE FMDV of the Picornaviridae family is a pathogen of huge economic importance to the livestock industry due to its effect on the restriction of livestock movement and necessary control measures required following an outbreak. The study of FMDV capsid assembly, and picornavirus capsid assembly more generally, has tended to be focused upon the formation of capsids from pentameric intermediates or the immediate cotranslational modification of the capsid precursor protein. Here, we describe a system to analyze the early stages of FMDV pentameric capsid intermediate assembly and demonstrate a novel requirement for the cellular chaperone hsp90 in the formation of these pentameric intermediates. We show the added complexity involved for this process to occur, which could be the basis for a novel antiviral control mechanism for FMDV. American Society for Microbiology 2018-02-12 /pmc/articles/PMC5809743/ /pubmed/29212943 http://dx.doi.org/10.1128/JVI.01415-17 Text en Copyright © 2018 Newman et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Structure and Assembly Newman, Joseph Asfor, Amin S. Berryman, Stephen Jackson, Terry Curry, Stephen Tuthill, Tobias J. The Cellular Chaperone Heat Shock Protein 90 Is Required for Foot-and-Mouth Disease Virus Capsid Precursor Processing and Assembly of Capsid Pentamers |
title | The Cellular Chaperone Heat Shock Protein 90 Is Required for Foot-and-Mouth Disease Virus Capsid Precursor Processing and Assembly of Capsid Pentamers |
title_full | The Cellular Chaperone Heat Shock Protein 90 Is Required for Foot-and-Mouth Disease Virus Capsid Precursor Processing and Assembly of Capsid Pentamers |
title_fullStr | The Cellular Chaperone Heat Shock Protein 90 Is Required for Foot-and-Mouth Disease Virus Capsid Precursor Processing and Assembly of Capsid Pentamers |
title_full_unstemmed | The Cellular Chaperone Heat Shock Protein 90 Is Required for Foot-and-Mouth Disease Virus Capsid Precursor Processing and Assembly of Capsid Pentamers |
title_short | The Cellular Chaperone Heat Shock Protein 90 Is Required for Foot-and-Mouth Disease Virus Capsid Precursor Processing and Assembly of Capsid Pentamers |
title_sort | cellular chaperone heat shock protein 90 is required for foot-and-mouth disease virus capsid precursor processing and assembly of capsid pentamers |
topic | Structure and Assembly |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5809743/ https://www.ncbi.nlm.nih.gov/pubmed/29212943 http://dx.doi.org/10.1128/JVI.01415-17 |
work_keys_str_mv | AT newmanjoseph thecellularchaperoneheatshockprotein90isrequiredforfootandmouthdiseaseviruscapsidprecursorprocessingandassemblyofcapsidpentamers AT asforamins thecellularchaperoneheatshockprotein90isrequiredforfootandmouthdiseaseviruscapsidprecursorprocessingandassemblyofcapsidpentamers AT berrymanstephen thecellularchaperoneheatshockprotein90isrequiredforfootandmouthdiseaseviruscapsidprecursorprocessingandassemblyofcapsidpentamers AT jacksonterry thecellularchaperoneheatshockprotein90isrequiredforfootandmouthdiseaseviruscapsidprecursorprocessingandassemblyofcapsidpentamers AT currystephen thecellularchaperoneheatshockprotein90isrequiredforfootandmouthdiseaseviruscapsidprecursorprocessingandassemblyofcapsidpentamers AT tuthilltobiasj thecellularchaperoneheatshockprotein90isrequiredforfootandmouthdiseaseviruscapsidprecursorprocessingandassemblyofcapsidpentamers AT newmanjoseph cellularchaperoneheatshockprotein90isrequiredforfootandmouthdiseaseviruscapsidprecursorprocessingandassemblyofcapsidpentamers AT asforamins cellularchaperoneheatshockprotein90isrequiredforfootandmouthdiseaseviruscapsidprecursorprocessingandassemblyofcapsidpentamers AT berrymanstephen cellularchaperoneheatshockprotein90isrequiredforfootandmouthdiseaseviruscapsidprecursorprocessingandassemblyofcapsidpentamers AT jacksonterry cellularchaperoneheatshockprotein90isrequiredforfootandmouthdiseaseviruscapsidprecursorprocessingandassemblyofcapsidpentamers AT currystephen cellularchaperoneheatshockprotein90isrequiredforfootandmouthdiseaseviruscapsidprecursorprocessingandassemblyofcapsidpentamers AT tuthilltobiasj cellularchaperoneheatshockprotein90isrequiredforfootandmouthdiseaseviruscapsidprecursorprocessingandassemblyofcapsidpentamers |