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Identification of a novel autoantibody against self-vimentin specific in secondary Sjögren’s syndrome
BACKGROUND: Sjögren’s syndrome (SS) is a primary autoimmune disease (pSS) or secondarily associated with other autoimmune diseases (sSS). The mechanisms underlying immune dysregulation in this syndrome remain unknown, and clinically it is difficult to diagnose owing to a lack of specific biomarkers....
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5810024/ https://www.ncbi.nlm.nih.gov/pubmed/29433534 http://dx.doi.org/10.1186/s13075-017-1508-5 |
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author | Li, Yu-Hui Gao, Ya-Ping Dong, Jie Shi, Lian-Jie Sun, Xiao-Lin Li, Ru Zhang, Xue-Wu Liu, Yu Long, Li He, Jing Zhong, Qun-Jie Morand, Eric Yang, Guang Li, Zhan-Guo |
author_facet | Li, Yu-Hui Gao, Ya-Ping Dong, Jie Shi, Lian-Jie Sun, Xiao-Lin Li, Ru Zhang, Xue-Wu Liu, Yu Long, Li He, Jing Zhong, Qun-Jie Morand, Eric Yang, Guang Li, Zhan-Guo |
author_sort | Li, Yu-Hui |
collection | PubMed |
description | BACKGROUND: Sjögren’s syndrome (SS) is a primary autoimmune disease (pSS) or secondarily associated with other autoimmune diseases (sSS). The mechanisms underlying immune dysregulation in this syndrome remain unknown, and clinically it is difficult to diagnose owing to a lack of specific biomarkers. METHODS: We extracted immunoglobulins (Igs) from the sera of patients with sSS associated with rheumatoid arthritis (RA) and used them to screen a phage display library of peptides with random sequences. RESULTS: Our results show that an sSS-specific peptide, designated 3S-P, was recognized by sera of 68.2% (60 of 88) patients with sSS, 66.2% of patients with RA-sSS, and 76.5% of patients with systemic lupus erythematosus (SLE)-sSS. The anti-3S-P antibody was scarcely found in patients with pSS (1.8%), RA (1.3%), SLE (4.2%), ankylosing spondylitis (0%), and gout (3.3%), as well as in healthy donors (2%). The 3S-P-binding Igs (antibodies) were used to identify antigens from salivary glands and synovial tissues from patients with sSS. A putative target autoantigen expressed in the synovium and salivary gland recognized by anti-3S-P antibody was identified as self-vimentin. CONCLUSIONS: This novel autoantibody is highly specific in the diagnosis of sSS, and the underlying molecular mechanism of the disease might be epitope spreading involved with vimentin. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13075-017-1508-5) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5810024 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-58100242018-02-16 Identification of a novel autoantibody against self-vimentin specific in secondary Sjögren’s syndrome Li, Yu-Hui Gao, Ya-Ping Dong, Jie Shi, Lian-Jie Sun, Xiao-Lin Li, Ru Zhang, Xue-Wu Liu, Yu Long, Li He, Jing Zhong, Qun-Jie Morand, Eric Yang, Guang Li, Zhan-Guo Arthritis Res Ther Research Article BACKGROUND: Sjögren’s syndrome (SS) is a primary autoimmune disease (pSS) or secondarily associated with other autoimmune diseases (sSS). The mechanisms underlying immune dysregulation in this syndrome remain unknown, and clinically it is difficult to diagnose owing to a lack of specific biomarkers. METHODS: We extracted immunoglobulins (Igs) from the sera of patients with sSS associated with rheumatoid arthritis (RA) and used them to screen a phage display library of peptides with random sequences. RESULTS: Our results show that an sSS-specific peptide, designated 3S-P, was recognized by sera of 68.2% (60 of 88) patients with sSS, 66.2% of patients with RA-sSS, and 76.5% of patients with systemic lupus erythematosus (SLE)-sSS. The anti-3S-P antibody was scarcely found in patients with pSS (1.8%), RA (1.3%), SLE (4.2%), ankylosing spondylitis (0%), and gout (3.3%), as well as in healthy donors (2%). The 3S-P-binding Igs (antibodies) were used to identify antigens from salivary glands and synovial tissues from patients with sSS. A putative target autoantigen expressed in the synovium and salivary gland recognized by anti-3S-P antibody was identified as self-vimentin. CONCLUSIONS: This novel autoantibody is highly specific in the diagnosis of sSS, and the underlying molecular mechanism of the disease might be epitope spreading involved with vimentin. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13075-017-1508-5) contains supplementary material, which is available to authorized users. BioMed Central 2018-02-12 2018 /pmc/articles/PMC5810024/ /pubmed/29433534 http://dx.doi.org/10.1186/s13075-017-1508-5 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Li, Yu-Hui Gao, Ya-Ping Dong, Jie Shi, Lian-Jie Sun, Xiao-Lin Li, Ru Zhang, Xue-Wu Liu, Yu Long, Li He, Jing Zhong, Qun-Jie Morand, Eric Yang, Guang Li, Zhan-Guo Identification of a novel autoantibody against self-vimentin specific in secondary Sjögren’s syndrome |
title | Identification of a novel autoantibody against self-vimentin specific in secondary Sjögren’s syndrome |
title_full | Identification of a novel autoantibody against self-vimentin specific in secondary Sjögren’s syndrome |
title_fullStr | Identification of a novel autoantibody against self-vimentin specific in secondary Sjögren’s syndrome |
title_full_unstemmed | Identification of a novel autoantibody against self-vimentin specific in secondary Sjögren’s syndrome |
title_short | Identification of a novel autoantibody against self-vimentin specific in secondary Sjögren’s syndrome |
title_sort | identification of a novel autoantibody against self-vimentin specific in secondary sjögren’s syndrome |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5810024/ https://www.ncbi.nlm.nih.gov/pubmed/29433534 http://dx.doi.org/10.1186/s13075-017-1508-5 |
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