Single cell RNA sequencing of stem cell-derived retinal ganglion cells

We used single cell sequencing technology to characterize the transcriptomes of 1,174 human embryonic stem cell-derived retinal ganglion cells (RGCs) at the single cell level. The human embryonic stem cell line BRN3B-mCherry (A81-H7), was differentiated to RGCs using a guided differentiation approac...

Descripción completa

Detalles Bibliográficos
Autores principales: Daniszewski, Maciej, Senabouth, Anne, Nguyen, Quan H., Crombie, Duncan E., Lukowski, Samuel W., Kulkarni, Tejal, Sluch, Valentin M., Jabbari, Jafar S., Chamling, Xitiz, Zack, Donald J., Pébay, Alice, Powell, Joseph E., Hewitt, Alex W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2018
Materias:
Data Descriptor
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5810423/
https://www.ncbi.nlm.nih.gov/pubmed/29437159
http://dx.doi.org/10.1038/sdata.2018.13
_version_ 1783299750907346944
author Daniszewski, Maciej
Senabouth, Anne
Nguyen, Quan H.
Crombie, Duncan E.
Lukowski, Samuel W.
Kulkarni, Tejal
Sluch, Valentin M.
Jabbari, Jafar S.
Chamling, Xitiz
Zack, Donald J.
Pébay, Alice
Powell, Joseph E.
Hewitt, Alex W.
author_facet Daniszewski, Maciej
Senabouth, Anne
Nguyen, Quan H.
Crombie, Duncan E.
Lukowski, Samuel W.
Kulkarni, Tejal
Sluch, Valentin M.
Jabbari, Jafar S.
Chamling, Xitiz
Zack, Donald J.
Pébay, Alice
Powell, Joseph E.
Hewitt, Alex W.
author_sort Daniszewski, Maciej
collection PubMed
description We used single cell sequencing technology to characterize the transcriptomes of 1,174 human embryonic stem cell-derived retinal ganglion cells (RGCs) at the single cell level. The human embryonic stem cell line BRN3B-mCherry (A81-H7), was differentiated to RGCs using a guided differentiation approach. Cells were harvested at day 36 and prepared for single cell RNA sequencing. Our data indicates the presence of three distinct subpopulations of cells, with various degrees of maturity. One cluster of 288 cells showed increased expression of genes involved in axon guidance together with semaphorin interactions, cell-extracellular matrix interactions and ECM proteoglycans, suggestive of a more mature RGC phenotype.
format Online
Article
Text
id pubmed-5810423
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-58104232018-02-21 Single cell RNA sequencing of stem cell-derived retinal ganglion cells Daniszewski, Maciej Senabouth, Anne Nguyen, Quan H. Crombie, Duncan E. Lukowski, Samuel W. Kulkarni, Tejal Sluch, Valentin M. Jabbari, Jafar S. Chamling, Xitiz Zack, Donald J. Pébay, Alice Powell, Joseph E. Hewitt, Alex W. Sci Data Data Descriptor We used single cell sequencing technology to characterize the transcriptomes of 1,174 human embryonic stem cell-derived retinal ganglion cells (RGCs) at the single cell level. The human embryonic stem cell line BRN3B-mCherry (A81-H7), was differentiated to RGCs using a guided differentiation approach. Cells were harvested at day 36 and prepared for single cell RNA sequencing. Our data indicates the presence of three distinct subpopulations of cells, with various degrees of maturity. One cluster of 288 cells showed increased expression of genes involved in axon guidance together with semaphorin interactions, cell-extracellular matrix interactions and ECM proteoglycans, suggestive of a more mature RGC phenotype. Nature Publishing Group 2018-02-13 /pmc/articles/PMC5810423/ /pubmed/29437159 http://dx.doi.org/10.1038/sdata.2018.13 Text en Copyright © 2018, The Author(s) http://creativecommons.org/licenses/by/4.0/ Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ The Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the metadata files made available in this article.
spellingShingle Data Descriptor
Daniszewski, Maciej
Senabouth, Anne
Nguyen, Quan H.
Crombie, Duncan E.
Lukowski, Samuel W.
Kulkarni, Tejal
Sluch, Valentin M.
Jabbari, Jafar S.
Chamling, Xitiz
Zack, Donald J.
Pébay, Alice
Powell, Joseph E.
Hewitt, Alex W.
Single cell RNA sequencing of stem cell-derived retinal ganglion cells
title Single cell RNA sequencing of stem cell-derived retinal ganglion cells
title_full Single cell RNA sequencing of stem cell-derived retinal ganglion cells
title_fullStr Single cell RNA sequencing of stem cell-derived retinal ganglion cells
title_full_unstemmed Single cell RNA sequencing of stem cell-derived retinal ganglion cells
title_short Single cell RNA sequencing of stem cell-derived retinal ganglion cells
title_sort single cell rna sequencing of stem cell-derived retinal ganglion cells
topic Data Descriptor
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5810423/
https://www.ncbi.nlm.nih.gov/pubmed/29437159
http://dx.doi.org/10.1038/sdata.2018.13
work_keys_str_mv AT daniszewskimaciej singlecellrnasequencingofstemcellderivedretinalganglioncells
AT senabouthanne singlecellrnasequencingofstemcellderivedretinalganglioncells
AT nguyenquanh singlecellrnasequencingofstemcellderivedretinalganglioncells
AT crombieduncane singlecellrnasequencingofstemcellderivedretinalganglioncells
AT lukowskisamuelw singlecellrnasequencingofstemcellderivedretinalganglioncells
AT kulkarnitejal singlecellrnasequencingofstemcellderivedretinalganglioncells
AT sluchvalentinm singlecellrnasequencingofstemcellderivedretinalganglioncells
AT jabbarijafars singlecellrnasequencingofstemcellderivedretinalganglioncells
AT chamlingxitiz singlecellrnasequencingofstemcellderivedretinalganglioncells
AT zackdonaldj singlecellrnasequencingofstemcellderivedretinalganglioncells
AT pebayalice singlecellrnasequencingofstemcellderivedretinalganglioncells
AT powelljosephe singlecellrnasequencingofstemcellderivedretinalganglioncells
AT hewittalexw singlecellrnasequencingofstemcellderivedretinalganglioncells

Ejemplares similares