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Genetic diversity and cross-species transmission of kobuviruses in Vietnam

Cross-species transmission of viruses poses a sustained threat to public health. Due to increased contact between humans and other animal species the possibility exists for cross-species transmissions and ensuing disease outbreaks. By using conventional PCR amplification and next generation sequenci...

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Autores principales: Lu, Lu, Van Dung, Nguyen, Ivens, Alasdair, Bogaardt, Carlijn, O’Toole, Aine, Bryant, Juliet E, Carrique-Mas, Juan, Van Cuong, Nguyen, Anh, Pham Hong, Rabaa, Maia A, Tue, Ngo Tri, Thwaites, Guy E, Baker, Stephen, Simmonds, Peter, Woolhouse, Mark Ej
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5810437/
https://www.ncbi.nlm.nih.gov/pubmed/29449965
http://dx.doi.org/10.1093/ve/vey002
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author Lu, Lu
Van Dung, Nguyen
Ivens, Alasdair
Bogaardt, Carlijn
O’Toole, Aine
Bryant, Juliet E
Carrique-Mas, Juan
Van Cuong, Nguyen
Anh, Pham Hong
Rabaa, Maia A
Tue, Ngo Tri
Thwaites, Guy E
Baker, Stephen
Simmonds, Peter
Woolhouse, Mark Ej
author_facet Lu, Lu
Van Dung, Nguyen
Ivens, Alasdair
Bogaardt, Carlijn
O’Toole, Aine
Bryant, Juliet E
Carrique-Mas, Juan
Van Cuong, Nguyen
Anh, Pham Hong
Rabaa, Maia A
Tue, Ngo Tri
Thwaites, Guy E
Baker, Stephen
Simmonds, Peter
Woolhouse, Mark Ej
author_sort Lu, Lu
collection PubMed
description Cross-species transmission of viruses poses a sustained threat to public health. Due to increased contact between humans and other animal species the possibility exists for cross-species transmissions and ensuing disease outbreaks. By using conventional PCR amplification and next generation sequencing, we obtained 130 partial or full genome kobuvirus sequences from humans in a sentinel cohort in Vietnam and various mammalian hosts including bats, rodents, pigs, cats, and civets. The evolution of kobuviruses in different hosts was analysed using Bayesian phylogenetic methods. We estimated and compared time of origin of kobuviruses in different host orders; we also examined the cross-species transmission of kobuviruses within the same host order and between different host orders. Our data provide new knowledge of rodent and bat kobuviruses, which are most closely related to human kobuviruses. The novel bat kobuviruses isolated from bat roosts in Southern Vietnam were genetically distinct from previously described bat kobuviruses, but closely related to kobuviruses found in rodents. We additionally found evidence of frequent cross-species transmissions of kobuviruses within rodents. Overall, our phylogenetic analyses reveal multiple cross-species transmissions both within and among mammalian species, which increases our understanding of kobuviruses genetic diversity and the complexity of their evolutionary history.
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spelling pubmed-58104372018-02-15 Genetic diversity and cross-species transmission of kobuviruses in Vietnam Lu, Lu Van Dung, Nguyen Ivens, Alasdair Bogaardt, Carlijn O’Toole, Aine Bryant, Juliet E Carrique-Mas, Juan Van Cuong, Nguyen Anh, Pham Hong Rabaa, Maia A Tue, Ngo Tri Thwaites, Guy E Baker, Stephen Simmonds, Peter Woolhouse, Mark Ej Virus Evol Research Article Cross-species transmission of viruses poses a sustained threat to public health. Due to increased contact between humans and other animal species the possibility exists for cross-species transmissions and ensuing disease outbreaks. By using conventional PCR amplification and next generation sequencing, we obtained 130 partial or full genome kobuvirus sequences from humans in a sentinel cohort in Vietnam and various mammalian hosts including bats, rodents, pigs, cats, and civets. The evolution of kobuviruses in different hosts was analysed using Bayesian phylogenetic methods. We estimated and compared time of origin of kobuviruses in different host orders; we also examined the cross-species transmission of kobuviruses within the same host order and between different host orders. Our data provide new knowledge of rodent and bat kobuviruses, which are most closely related to human kobuviruses. The novel bat kobuviruses isolated from bat roosts in Southern Vietnam were genetically distinct from previously described bat kobuviruses, but closely related to kobuviruses found in rodents. We additionally found evidence of frequent cross-species transmissions of kobuviruses within rodents. Overall, our phylogenetic analyses reveal multiple cross-species transmissions both within and among mammalian species, which increases our understanding of kobuviruses genetic diversity and the complexity of their evolutionary history. Oxford University Press 2018-02-13 /pmc/articles/PMC5810437/ /pubmed/29449965 http://dx.doi.org/10.1093/ve/vey002 Text en © The Author(s) 2018. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Research Article
Lu, Lu
Van Dung, Nguyen
Ivens, Alasdair
Bogaardt, Carlijn
O’Toole, Aine
Bryant, Juliet E
Carrique-Mas, Juan
Van Cuong, Nguyen
Anh, Pham Hong
Rabaa, Maia A
Tue, Ngo Tri
Thwaites, Guy E
Baker, Stephen
Simmonds, Peter
Woolhouse, Mark Ej
Genetic diversity and cross-species transmission of kobuviruses in Vietnam
title Genetic diversity and cross-species transmission of kobuviruses in Vietnam
title_full Genetic diversity and cross-species transmission of kobuviruses in Vietnam
title_fullStr Genetic diversity and cross-species transmission of kobuviruses in Vietnam
title_full_unstemmed Genetic diversity and cross-species transmission of kobuviruses in Vietnam
title_short Genetic diversity and cross-species transmission of kobuviruses in Vietnam
title_sort genetic diversity and cross-species transmission of kobuviruses in vietnam
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5810437/
https://www.ncbi.nlm.nih.gov/pubmed/29449965
http://dx.doi.org/10.1093/ve/vey002
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