Cargando…

A fence barrier method of leading edge cell capture for explorative biochemical research

The scratch or wound-healing assay is used ubiquitously for investigating re-epithelialisation and has already revealed the importance of cells comprising the leading edge of healing epithelial wounds. However it is currently limited to studying the effect of known biochemical agents on the tissue o...

Descripción completa

Detalles Bibliográficos
Autores principales: Wager, Lucas J., Murray, Rachael Z., Thompson, Erik W., Leavesley, David I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5810785/
https://www.ncbi.nlm.nih.gov/pubmed/28276927
http://dx.doi.org/10.1080/19336918.2016.1269997
_version_ 1783299776298614784
author Wager, Lucas J.
Murray, Rachael Z.
Thompson, Erik W.
Leavesley, David I.
author_facet Wager, Lucas J.
Murray, Rachael Z.
Thompson, Erik W.
Leavesley, David I.
author_sort Wager, Lucas J.
collection PubMed
description The scratch or wound-healing assay is used ubiquitously for investigating re-epithelialisation and has already revealed the importance of cells comprising the leading edge of healing epithelial wounds. However it is currently limited to studying the effect of known biochemical agents on the tissue of choice. Here we present an adaptation that extends the utility of this model to encompass the collection of cells from the leading edge of migrating epithelial sheets making available explorative biochemical analyses. The method is scalable and does not require expensive apparatus, making it suitable for large and small laboratories alike. We detail the application of our method and exemplify proof of principle data derived from primary human keratinocyte cultures.
format Online
Article
Text
id pubmed-5810785
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Taylor & Francis
record_format MEDLINE/PubMed
spelling pubmed-58107852018-02-15 A fence barrier method of leading edge cell capture for explorative biochemical research Wager, Lucas J. Murray, Rachael Z. Thompson, Erik W. Leavesley, David I. Cell Adh Migr Technical Paper The scratch or wound-healing assay is used ubiquitously for investigating re-epithelialisation and has already revealed the importance of cells comprising the leading edge of healing epithelial wounds. However it is currently limited to studying the effect of known biochemical agents on the tissue of choice. Here we present an adaptation that extends the utility of this model to encompass the collection of cells from the leading edge of migrating epithelial sheets making available explorative biochemical analyses. The method is scalable and does not require expensive apparatus, making it suitable for large and small laboratories alike. We detail the application of our method and exemplify proof of principle data derived from primary human keratinocyte cultures. Taylor & Francis 2017-02-17 /pmc/articles/PMC5810785/ /pubmed/28276927 http://dx.doi.org/10.1080/19336918.2016.1269997 Text en © 2017 The Author(s). Published with license by Taylor & Francis http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.
spellingShingle Technical Paper
Wager, Lucas J.
Murray, Rachael Z.
Thompson, Erik W.
Leavesley, David I.
A fence barrier method of leading edge cell capture for explorative biochemical research
title A fence barrier method of leading edge cell capture for explorative biochemical research
title_full A fence barrier method of leading edge cell capture for explorative biochemical research
title_fullStr A fence barrier method of leading edge cell capture for explorative biochemical research
title_full_unstemmed A fence barrier method of leading edge cell capture for explorative biochemical research
title_short A fence barrier method of leading edge cell capture for explorative biochemical research
title_sort fence barrier method of leading edge cell capture for explorative biochemical research
topic Technical Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5810785/
https://www.ncbi.nlm.nih.gov/pubmed/28276927
http://dx.doi.org/10.1080/19336918.2016.1269997
work_keys_str_mv AT wagerlucasj afencebarriermethodofleadingedgecellcaptureforexplorativebiochemicalresearch
AT murrayrachaelz afencebarriermethodofleadingedgecellcaptureforexplorativebiochemicalresearch
AT thompsonerikw afencebarriermethodofleadingedgecellcaptureforexplorativebiochemicalresearch
AT leavesleydavidi afencebarriermethodofleadingedgecellcaptureforexplorativebiochemicalresearch
AT wagerlucasj fencebarriermethodofleadingedgecellcaptureforexplorativebiochemicalresearch
AT murrayrachaelz fencebarriermethodofleadingedgecellcaptureforexplorativebiochemicalresearch
AT thompsonerikw fencebarriermethodofleadingedgecellcaptureforexplorativebiochemicalresearch
AT leavesleydavidi fencebarriermethodofleadingedgecellcaptureforexplorativebiochemicalresearch