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A fence barrier method of leading edge cell capture for explorative biochemical research
The scratch or wound-healing assay is used ubiquitously for investigating re-epithelialisation and has already revealed the importance of cells comprising the leading edge of healing epithelial wounds. However it is currently limited to studying the effect of known biochemical agents on the tissue o...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5810785/ https://www.ncbi.nlm.nih.gov/pubmed/28276927 http://dx.doi.org/10.1080/19336918.2016.1269997 |
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author | Wager, Lucas J. Murray, Rachael Z. Thompson, Erik W. Leavesley, David I. |
author_facet | Wager, Lucas J. Murray, Rachael Z. Thompson, Erik W. Leavesley, David I. |
author_sort | Wager, Lucas J. |
collection | PubMed |
description | The scratch or wound-healing assay is used ubiquitously for investigating re-epithelialisation and has already revealed the importance of cells comprising the leading edge of healing epithelial wounds. However it is currently limited to studying the effect of known biochemical agents on the tissue of choice. Here we present an adaptation that extends the utility of this model to encompass the collection of cells from the leading edge of migrating epithelial sheets making available explorative biochemical analyses. The method is scalable and does not require expensive apparatus, making it suitable for large and small laboratories alike. We detail the application of our method and exemplify proof of principle data derived from primary human keratinocyte cultures. |
format | Online Article Text |
id | pubmed-5810785 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-58107852018-02-15 A fence barrier method of leading edge cell capture for explorative biochemical research Wager, Lucas J. Murray, Rachael Z. Thompson, Erik W. Leavesley, David I. Cell Adh Migr Technical Paper The scratch or wound-healing assay is used ubiquitously for investigating re-epithelialisation and has already revealed the importance of cells comprising the leading edge of healing epithelial wounds. However it is currently limited to studying the effect of known biochemical agents on the tissue of choice. Here we present an adaptation that extends the utility of this model to encompass the collection of cells from the leading edge of migrating epithelial sheets making available explorative biochemical analyses. The method is scalable and does not require expensive apparatus, making it suitable for large and small laboratories alike. We detail the application of our method and exemplify proof of principle data derived from primary human keratinocyte cultures. Taylor & Francis 2017-02-17 /pmc/articles/PMC5810785/ /pubmed/28276927 http://dx.doi.org/10.1080/19336918.2016.1269997 Text en © 2017 The Author(s). Published with license by Taylor & Francis http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted. |
spellingShingle | Technical Paper Wager, Lucas J. Murray, Rachael Z. Thompson, Erik W. Leavesley, David I. A fence barrier method of leading edge cell capture for explorative biochemical research |
title | A fence barrier method of leading edge cell capture for explorative biochemical research |
title_full | A fence barrier method of leading edge cell capture for explorative biochemical research |
title_fullStr | A fence barrier method of leading edge cell capture for explorative biochemical research |
title_full_unstemmed | A fence barrier method of leading edge cell capture for explorative biochemical research |
title_short | A fence barrier method of leading edge cell capture for explorative biochemical research |
title_sort | fence barrier method of leading edge cell capture for explorative biochemical research |
topic | Technical Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5810785/ https://www.ncbi.nlm.nih.gov/pubmed/28276927 http://dx.doi.org/10.1080/19336918.2016.1269997 |
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