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Muscle function decline and mitochondria changes in middle age precede sarcopenia in mice

Sarcopenia is the degenerative loss of muscle mass and strength with aging. Although a role of mitochondrial metabolism in muscle function and in the development of many diseases has been described, the role of mitochondrial topology and dynamics in the process of muscle aging is not fully understoo...

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Autores principales: del Campo, Andrea, Contreras-Hernández, Ignacio, Castro-Sepúlveda, Mauricio, Campos, Cristian A., Figueroa, Reinaldo, Tevy, María Florencia, Eisner, Verónica, Casas, Mariana, Jaimovich, Enrique
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5811241/
https://www.ncbi.nlm.nih.gov/pubmed/29302020
http://dx.doi.org/10.18632/aging.101358
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author del Campo, Andrea
Contreras-Hernández, Ignacio
Castro-Sepúlveda, Mauricio
Campos, Cristian A.
Figueroa, Reinaldo
Tevy, María Florencia
Eisner, Verónica
Casas, Mariana
Jaimovich, Enrique
author_facet del Campo, Andrea
Contreras-Hernández, Ignacio
Castro-Sepúlveda, Mauricio
Campos, Cristian A.
Figueroa, Reinaldo
Tevy, María Florencia
Eisner, Verónica
Casas, Mariana
Jaimovich, Enrique
author_sort del Campo, Andrea
collection PubMed
description Sarcopenia is the degenerative loss of muscle mass and strength with aging. Although a role of mitochondrial metabolism in muscle function and in the development of many diseases has been described, the role of mitochondrial topology and dynamics in the process of muscle aging is not fully understood. This work shows a time line of changes in both mitochondrial distribution and skeletal muscle function during mice lifespan. We isolated muscle fibers from flexor digitorum brevis of mice of different ages. A fusion-like phenotype of mitochondria, together with a change in orientation perpendicular to the fiber axis was evident in the Adult group compared to Juvenile and Older groups. Moreover, an increase in the contact area between sarcoplasmic reticulum and mitochondria was evident in the same group. Together with the morphological changes, mitochondrial Ca(2+) resting levels were reduced at age 10-14 months and significantly increased in the Older group. This was consistent with a reduced number of mitochondria-to-jSR pairs in the Older group compared to the Juvenile. Our results support the idea of several age-dependent changes in mitochondria that are accentuated in midlife prior to a complete sarcopenic phenotype.
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spelling pubmed-58112412018-02-19 Muscle function decline and mitochondria changes in middle age precede sarcopenia in mice del Campo, Andrea Contreras-Hernández, Ignacio Castro-Sepúlveda, Mauricio Campos, Cristian A. Figueroa, Reinaldo Tevy, María Florencia Eisner, Verónica Casas, Mariana Jaimovich, Enrique Aging (Albany NY) Research Paper Sarcopenia is the degenerative loss of muscle mass and strength with aging. Although a role of mitochondrial metabolism in muscle function and in the development of many diseases has been described, the role of mitochondrial topology and dynamics in the process of muscle aging is not fully understood. This work shows a time line of changes in both mitochondrial distribution and skeletal muscle function during mice lifespan. We isolated muscle fibers from flexor digitorum brevis of mice of different ages. A fusion-like phenotype of mitochondria, together with a change in orientation perpendicular to the fiber axis was evident in the Adult group compared to Juvenile and Older groups. Moreover, an increase in the contact area between sarcoplasmic reticulum and mitochondria was evident in the same group. Together with the morphological changes, mitochondrial Ca(2+) resting levels were reduced at age 10-14 months and significantly increased in the Older group. This was consistent with a reduced number of mitochondria-to-jSR pairs in the Older group compared to the Juvenile. Our results support the idea of several age-dependent changes in mitochondria that are accentuated in midlife prior to a complete sarcopenic phenotype. Impact Journals 2018-01-04 /pmc/articles/PMC5811241/ /pubmed/29302020 http://dx.doi.org/10.18632/aging.101358 Text en Copyright © 2018 del Campo et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY) 3.0 License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
del Campo, Andrea
Contreras-Hernández, Ignacio
Castro-Sepúlveda, Mauricio
Campos, Cristian A.
Figueroa, Reinaldo
Tevy, María Florencia
Eisner, Verónica
Casas, Mariana
Jaimovich, Enrique
Muscle function decline and mitochondria changes in middle age precede sarcopenia in mice
title Muscle function decline and mitochondria changes in middle age precede sarcopenia in mice
title_full Muscle function decline and mitochondria changes in middle age precede sarcopenia in mice
title_fullStr Muscle function decline and mitochondria changes in middle age precede sarcopenia in mice
title_full_unstemmed Muscle function decline and mitochondria changes in middle age precede sarcopenia in mice
title_short Muscle function decline and mitochondria changes in middle age precede sarcopenia in mice
title_sort muscle function decline and mitochondria changes in middle age precede sarcopenia in mice
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5811241/
https://www.ncbi.nlm.nih.gov/pubmed/29302020
http://dx.doi.org/10.18632/aging.101358
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