Cargando…

Detecting RNA base methylations in single cells by in situ hybridization

Methylated bases in tRNA, rRNA and mRNA control a variety of cellular processes, including protein synthesis, antimicrobial resistance and gene expression. Currently, bulk methods that report the average methylation state of ~10(4)–10(7) cells are used to detect these modifications, obscuring potent...

Descripción completa

Detalles Bibliográficos
Autores principales:	Ranasinghe, Rohan T., Challand, Martin R., Ganzinger, Kristina A., Lewis, Benjamin W., Softley, Charlotte, Schmied, Wolfgang H., Horrocks, Mathew H., Shivji, Nadia, Chin, Jason W., Spencer, James, Klenerman, David
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2018
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5811446/ https://www.ncbi.nlm.nih.gov/pubmed/29440632 http://dx.doi.org/10.1038/s41467-017-02714-7

Ejemplares similares

A mechanistic model of tau amyloid aggregation based on direct observation of oligomers
por: Shammas, Sarah L., et al.
Publicado: (2015)

Single-Molecule Imaging Reveals that Small Amyloid-β(1–42) Oligomers Interact with the Cellular Prion Protein (PrP(C))
por: Ganzinger, Kristina A, et al.
Publicado: (2014)

Cysteine Methylation Controls Radical Generation in the Cfr Radical AdoMet rRNA Methyltransferase
por: Challand, Martin R., et al.
Publicado: (2013)

Multi-dimensional super-resolution imaging enables surface hydrophobicity mapping
por: Bongiovanni, Marie N., et al.
Publicado: (2016)

Self-Assembling Protein Surfaces for In Situ Capture of Cell-Free-Synthesized Proteins
por: Thornton, Ella Lucille, et al.
Publicado: (2022)

Lipid peroxidation is essential for α‐synuclein‐induced cell death
por: Angelova, Plamena R., et al.
Publicado: (2015)

Extrinsic Amyloid-Binding Dyes for Detection of Individual Protein Aggregates in Solution
por: Taylor, Christopher G., et al.
Publicado: (2018)

Prader-Willi syndrome: Methylation study or fluorescence in situ hybridization first?
por: Hamzi, Khalil, et al.
Publicado: (2010)

Single-Molecule Measurements of Transient Biomolecular Complexes through Microfluidic Dilution
por: Horrocks, Mathew H., et al.
Publicado: (2013)

In situ hybridization of viral nucleic acids in tumour cells.
por: McDougall, J. K., et al.
Publicado: (1973)

Nanoscopic Characterisation of Individual Endogenous Protein Aggregates in Human Neuronal Cells
por: Whiten, Daniel R., et al.
Publicado: (2018)

Osteocalcin expression in primary bone tumors--in situ hybridization and immunohistochemical study.
por: Park, Y. K., et al.
Publicado: (1995)

Super-resolution imaging reveals α-synuclein seeded aggregation in SH-SY5Y cells
por: Sang, Jason C., et al.
Publicado: (2021)

Five cases of cytomegalovirus infection detected by in situ hybridization and antigenemia assay.
por: Yoo, J. H., et al.
Publicado: (1994)

Ribosome Subunit Stapling for Orthogonal Translation in E. coli
por: Fried, Stephen D, et al.
Publicado: (2015)

Efficient Multisite Unnatural Amino Acid Incorporation in Mammalian Cells via Optimized Pyrrolysyl tRNA Synthetase/tRNA Expression and Engineered eRF1
por: Schmied, Wolfgang H., et al.
Publicado: (2014)

Ribosome Subunit Stapling for Orthogonal Translation in E. coli
por: Fried, Stephen D., et al.
Publicado: (2015)

In situ hybridization protocols /
Publicado: (2006)

A Quantitative Comparison of Single-Dye Tracking Analysis Tools Using Monte Carlo Simulations
por: Weimann, Laura, et al.
Publicado: (2013)

Fluorescence in situ hybridization (FISH) and cell sorting of living bacteria
por: Batani, Giampiero, et al.
Publicado: (2019)

Construction of repeat-free fluorescence in situ hybridization probes
por: Swennenhuis, Joost F., et al.
Publicado: (2012)

Editorial: From reconstituting minimal cell-cell signaling systems to bioinspired synthetic communication networks
por: Ganzinger, Kristina A., et al.
Publicado: (2022)

Interpretation in Clinical Biochemistry: An External Quality Assurance Scheme
por: Challand, Gordon, et al.
Publicado: (2004)

Individual aggregates of amyloid beta induce temporary calcium influx through the cell membrane of neuronal cells
por: Drews, Anna, et al.
Publicado: (2016)

In Situ Synthesis of Hybrid Inorganic–Polymer Nanocomposites
por: Adnan, Mohammed M., et al.
Publicado: (2018)

Molecular Pathology—In Situ Hybridization
por: Sterchi, Diane L.
Publicado: (2008)

EBV in situ hybridization study for non-Hodgkin's lymphomas.
por: Ko, Y. H., et al.
Publicado: (1994)

Single-molecule FRET studies on alpha-synuclein oligomerization of Parkinson’s disease genetically related mutants
por: Tosatto, Laura, et al.
Publicado: (2015)

Hsp70 Inhibits the Nucleation and Elongation of Tau and Sequesters Tau Aggregates with High Affinity
por: Kundel, Franziska, et al.
Publicado: (2018)

Single-Molecule Imaging of Individual Amyloid Protein Aggregates in Human Biofluids
por: Horrocks, Mathew H., et al.
Publicado: (2016)

Ca(2+) is a key factor in α-synuclein-induced neurotoxicity
por: Angelova, Plamena R., et al.
Publicado: (2016)

Synthetic cell division via membrane-transforming molecular assemblies
por: Kretschmer, Simon, et al.
Publicado: (2019)

In Situ Hybridization of Viral Nucleic Acids
Publicado: (1975)

Comparative in situ hybridization protocols in zebrafish
por: Dunn, Krista, et al.
Publicado: (2022)

Revision total hip replacement: predictors of blood loss, transfusion requirements, and length of hospitalisation
por: Mahadevan, Devendra, et al.
Publicado: (2010)

Digoxigenin-Labeled In Situ Hybridization for the Detection of Streptococcus suis DNA in Polyserositis and a Comparison with Biotinylated In Situ Hybridization
por: KANG, Ikjae, et al.
Publicado: (2013)

Comparison of Fluorescence In Situ Hybridization and Chromogenic In Situ Hybridization for Low and High Throughput HER2 Genetic Testing
por: Poulsen, Tim S., et al.
Publicado: (2013)

Localization of hepatitis B virus DNA in hepatocellular carcinoma by polymerase chain reaction in situ hybridization.
por: Shin, Y. J., et al.
Publicado: (1998)

Multiplexed analysis of fixed tissue RNA using Ligation in situ Hybridization
por: Credle, Joel J., et al.
Publicado: (2017)

The K2: Open-source simultaneous triple-color TIRF microscope for live-cell and single-molecule imaging
por: Niederauer, Christian, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_i6ec0j46j5ld8ejp27bucbrr51