Cargando…

Combined aptamer and transcriptome sequencing of single cells

The transcriptome and proteome encode distinct information that is important for characterizing heterogeneous biological systems. We demonstrate a method to simultaneously characterize the transcriptomes and proteomes of single cells at high throughput using aptamer probes and droplet-based single c...

Descripción completa

Detalles Bibliográficos
Autores principales:	Delley, Cyrille L., Liu, Leqian, Sarhan, Maen F., Abate, Adam R.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2018
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5811598/ https://www.ncbi.nlm.nih.gov/pubmed/29440771 http://dx.doi.org/10.1038/s41598-018-21153-y

Ejemplares similares

RNA-aptamers-in-droplets (RAPID) high-throughput screening for secretory phenotypes
por: Abatemarco, Joseph, et al.
Publicado: (2017)

Modular barcode beads for microfluidic single cell genomics
por: Delley, Cyrille L., et al.
Publicado: (2021)

Joint profiling of DNA and proteins in single cells to dissect genotype-phenotype associations in leukemia
por: Demaree, Benjamin, et al.
Publicado: (2021)

Linked optical and gene expression profiling of single cells at high-throughput
por: Zhang, Jesse Q., et al.
Publicado: (2020)

Multiomic Single Cell Sequencing Identifies Stemlike Nature of Mixed Phenotype Acute Leukemia and Provides Novel Risk Stratification
por: Peretz, Cheryl A. C., et al.
Publicado: (2023)

Reference-based analysis of lung single-cell sequencing reveals a transitional profibrotic macrophage
por: Aran, Dvir, et al.
Publicado: (2019)

Characterizing cell interactions at scale with made-to-order droplet ensembles (MODEs)
por: Madrigal, Justin L., et al.
Publicado: (2022)

Combining capillary electrophoresis and next-generation sequencing for aptamer selection
por: Riley, Kathryn R., et al.
Publicado: (2015)

Droplet microfluidic sequencing of HIV proviruses with their integration sites in patients receiving antiretroviral therapy
por: Sun, Chen, et al.
Publicado: (2022)

The pupylation pathway and its role in mycobacteria
por: Barandun, Jonas, et al.
Publicado: (2012)

Accurate bulk quantitation of droplet digital PCR
por: Sun, Chen, et al.
Publicado: (2021)

Genetic interaction mapping with microfluidic-based single cell sequencing
por: Haliburton, John R., et al.
Publicado: (2017)

Accurate Bulk Quantitation of Droplet Digital Polymerase Chain Reaction
por: Sun, Chen, et al.
Publicado: (2021)

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
por: Demaree, Benjamin, et al.
Publicado: (2018)

Electrostatic interactions guide substrate recognition of the prokaryotic ubiquitin-like protein ligase PafA
por: Block, Matthias F., et al.
Publicado: (2023)

Cell-SELEX Identifies a “Sticky” RNA Aptamer Sequence
por: Ray, Partha, et al.
Publicado: (2017)

Single cell mutation analysis of clonal evolution in myeloid malignancies
por: Miles, Linde A., et al.
Publicado: (2020)

G1/S restriction point coordinates phasic gene expression and cell differentiation
por: DeVeale, Brian, et al.
Publicado: (2022)

Cross-species single-cell transcriptomic analysis reveals pre-gastrulation developmental differences among pigs, monkeys, and humans
por: Liu, Tianbin, et al.
Publicado: (2021)

Droplet barcoding for massively parallel single-molecule deep sequencing
por: Lan, Freeman, et al.
Publicado: (2016)

Single-Step Selection of Bivalent Aptamers Validated by Comparison with SELEX Using High-Throughput Sequencing
por: Wilson, Robert, et al.
Publicado: (2014)

Draft Genome Sequence of the Oleaginous Yeast Yarrowia lipolytica PO1f, a Commonly Used Metabolic Engineering Host
por: Liu, Leqian, et al.
Publicado: (2014)

Prokaryotic ubiquitin-like protein remains intrinsically disordered when covalently attached to proteasomal target proteins
por: Barandun, Jonas, et al.
Publicado: (2017)

Bacterial Proteasome Activator Bpa (Rv3780) Is a Novel Ring-Shaped Interactor of the Mycobacterial Proteasome
por: Delley, Cyrille L., et al.
Publicado: (2014)

Optimization of Vancomycin Aptamer Sequence Length Increases the Sensitivity of Electrochemical, Aptamer-Based Sensors In Vivo
por: Shaver, Alexander, et al.
Publicado: (2022)

Microfluidics-free single-cell genomics with templated emulsification
por: Clark, Iain C., et al.
Publicado: (2023)

Single-round isolation of diverse RNA aptamers from a random sequence pool
por: Imashimizu, Masahiko, et al.
Publicado: (2018)

Nanopore sequencing of single-cell transcriptomes with scCOLOR-seq
por: Philpott, Martin, et al.
Publicado: (2021)

Digital droplet PCR accurately quantifies SARS-CoV-2 viral load from crude lysate without nucleic acid purification
por: Vasudevan, Harish N., et al.
Publicado: (2021)

Simultaneous multiplexed amplicon sequencing and transcriptome profiling in single cells
por: Saikia, Mridusmita, et al.
Publicado: (2018)

Aptamer Combined with Fluorescent Silica Nanoparticles for Detection of Hepatoma Cells
por: Hu, Zixi, et al.
Publicado: (2017)

Author Correction: Cross-species single-cell transcriptomic analysis reveals pre-gastrulation developmental differences among pigs, monkeys, and humans
por: Liu, Tianbin, et al.
Publicado: (2021)

Cell Wall Stress Depolarizes Cell Growth via Hyperactivation of Rho1
por: Delley, Pierre-Alain, et al.
Publicado: (1999)

Common Secondary and Tertiary Structural Features of Aptamer–Ligand Interaction Shared by RNA Aptamers with Different Primary Sequences
por: Ilgu, Muslum, et al.
Publicado: (2019)

The Regioselective Conjugation of the 15-nt Thrombin Aptamer with an Optimized Tripeptide Sequence Greatly Increases the Anticoagulant Activity of the Aptamer
por: Varizhuk, Irina V., et al.
Publicado: (2023)

Prediction of aptamer-protein interacting pairs using an ensemble classifier in combination with various protein sequence attributes
por: Zhang, Lina, et al.
Publicado: (2016)

Comparative analysis of sequencing technologies for single-cell transcriptomics
por: Natarajan, Kedar Nath, et al.
Publicado: (2019)

Massively Parallel Interrogation of Aptamer Sequence, Structure and Function
por: Fischer, Nicholas O., et al.
Publicado: (2008)

Single-cell transcriptome sequencing: recent advances and remaining challenges
por: Liu, Serena, et al.
Publicado: (2016)

Rapid Identification of Cell-Specific, Internalizing RNA Aptamers with Bioinformatics Analyses of a Cell-Based Aptamer Selection
por: Thiel, William H., et al.
Publicado: (2012)

Cannot write session to /tmp/vufind_sessions/sess_l0g84op90flt80jpgsengjl8cf