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Minocycline through attenuation of oxidative stress and inflammatory response reduces the neuropathic pain in a rat model of chronic constriction injury

OBJECTIVE(S): Several lines of evidence showed that minocycline possesses antioxidant and anti-inflammatory properties. This study aimed to demonstrate the effects of minocycline in rats subjected to chronic constriction injury (CCI). MATERIALS AND METHODS: In this study four groups (n = 6–8) of rat...

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Autores principales: Abbaszadeh, Abolfazl, Darabi, Saeideh, Hasanvand, Amin, Amini-khoei, Hossein, Abbasnezhad, Amir, Choghakhori, Razieh, Aaliehpour, Asghar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5811752/
https://www.ncbi.nlm.nih.gov/pubmed/29456810
http://dx.doi.org/10.22038/IJBMS.2017.24248.6053
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author Abbaszadeh, Abolfazl
Darabi, Saeideh
Hasanvand, Amin
Amini-khoei, Hossein
Abbasnezhad, Amir
Choghakhori, Razieh
Aaliehpour, Asghar
author_facet Abbaszadeh, Abolfazl
Darabi, Saeideh
Hasanvand, Amin
Amini-khoei, Hossein
Abbasnezhad, Amir
Choghakhori, Razieh
Aaliehpour, Asghar
author_sort Abbaszadeh, Abolfazl
collection PubMed
description OBJECTIVE(S): Several lines of evidence showed that minocycline possesses antioxidant and anti-inflammatory properties. This study aimed to demonstrate the effects of minocycline in rats subjected to chronic constriction injury (CCI). MATERIALS AND METHODS: In this study four groups (n = 6–8) of rats were used as follows: Sham, CCI, CCI + minocycline (MIN) 10 mg/Kg (IP) and CCI + MIN 30 mg/Kg (IP). On days 3, 7, 14, and 21 post-surgery hot-plate, acetone, and von Frey tests were carried out. Finally, Motor Nerve Conduction Velocity Evaluation (MNCV) assessment was performed and spinal cords were harvested in order to measure tissue concentrations of TNF_α, IL-1β, Glutathione peroxidase (GPx), Superoxide dismutase (SOD) and Malondialdehyde (MDA). Extent of perineural inflammation and damage around the sciatic nerve was histopathologically evaluated. RESULTS: Our results demonstrated that CCI significantly caused hyperalgesia and allodynia twenty-one days after CCI. MIN attenuated heat hyperalgesia, cold and mechanical allodynia and MNCV in animals. MIN also decreased the levels of TNF_α and IL-1β. Antioxidative enzymes (SOD, MDA, and GPx) were restored following MIN treatment. Our findings showed that MIN decreased perineural inflammation around the sciatic nerve. According to the results, the neuropathic pain reduced in the CCI hyperalgesia model using 30 mg/kg of minocycline. CONCLUSION: It is suggested that antinociceptive effects of minocycline might be mediated through the inhibition of inflammatory response and attenuation of oxidative stress.
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spelling pubmed-58117522018-02-16 Minocycline through attenuation of oxidative stress and inflammatory response reduces the neuropathic pain in a rat model of chronic constriction injury Abbaszadeh, Abolfazl Darabi, Saeideh Hasanvand, Amin Amini-khoei, Hossein Abbasnezhad, Amir Choghakhori, Razieh Aaliehpour, Asghar Iran J Basic Med Sci Original Article OBJECTIVE(S): Several lines of evidence showed that minocycline possesses antioxidant and anti-inflammatory properties. This study aimed to demonstrate the effects of minocycline in rats subjected to chronic constriction injury (CCI). MATERIALS AND METHODS: In this study four groups (n = 6–8) of rats were used as follows: Sham, CCI, CCI + minocycline (MIN) 10 mg/Kg (IP) and CCI + MIN 30 mg/Kg (IP). On days 3, 7, 14, and 21 post-surgery hot-plate, acetone, and von Frey tests were carried out. Finally, Motor Nerve Conduction Velocity Evaluation (MNCV) assessment was performed and spinal cords were harvested in order to measure tissue concentrations of TNF_α, IL-1β, Glutathione peroxidase (GPx), Superoxide dismutase (SOD) and Malondialdehyde (MDA). Extent of perineural inflammation and damage around the sciatic nerve was histopathologically evaluated. RESULTS: Our results demonstrated that CCI significantly caused hyperalgesia and allodynia twenty-one days after CCI. MIN attenuated heat hyperalgesia, cold and mechanical allodynia and MNCV in animals. MIN also decreased the levels of TNF_α and IL-1β. Antioxidative enzymes (SOD, MDA, and GPx) were restored following MIN treatment. Our findings showed that MIN decreased perineural inflammation around the sciatic nerve. According to the results, the neuropathic pain reduced in the CCI hyperalgesia model using 30 mg/kg of minocycline. CONCLUSION: It is suggested that antinociceptive effects of minocycline might be mediated through the inhibition of inflammatory response and attenuation of oxidative stress. Mashhad University of Medical Sciences 2018-02 /pmc/articles/PMC5811752/ /pubmed/29456810 http://dx.doi.org/10.22038/IJBMS.2017.24248.6053 Text en Copyright: © Iranian Journal of Basic Medical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Abbaszadeh, Abolfazl
Darabi, Saeideh
Hasanvand, Amin
Amini-khoei, Hossein
Abbasnezhad, Amir
Choghakhori, Razieh
Aaliehpour, Asghar
Minocycline through attenuation of oxidative stress and inflammatory response reduces the neuropathic pain in a rat model of chronic constriction injury
title Minocycline through attenuation of oxidative stress and inflammatory response reduces the neuropathic pain in a rat model of chronic constriction injury
title_full Minocycline through attenuation of oxidative stress and inflammatory response reduces the neuropathic pain in a rat model of chronic constriction injury
title_fullStr Minocycline through attenuation of oxidative stress and inflammatory response reduces the neuropathic pain in a rat model of chronic constriction injury
title_full_unstemmed Minocycline through attenuation of oxidative stress and inflammatory response reduces the neuropathic pain in a rat model of chronic constriction injury
title_short Minocycline through attenuation of oxidative stress and inflammatory response reduces the neuropathic pain in a rat model of chronic constriction injury
title_sort minocycline through attenuation of oxidative stress and inflammatory response reduces the neuropathic pain in a rat model of chronic constriction injury
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5811752/
https://www.ncbi.nlm.nih.gov/pubmed/29456810
http://dx.doi.org/10.22038/IJBMS.2017.24248.6053
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