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Phenotypical analysis of ectoenzymes CD39/CD73 and adenosine receptor 2A in CD4(+) CD25(high)Foxp3(+) regulatory T‐cells in psoriasis

BACKGROUND: CD39 and CD73 are two novel cell surface markers of CD25(high)Foxp3(+) regulatory T‐cells (Tregs). Concordant expression of these two ectoenzymes not only discriminate Tregs from other cell populations, but also generates pericellular adenosine, which has been reported to suppress prolif...

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Autores principales: Han, Ling, Sugiyama, Hideaki, Zhang, Qi, Yan, Kexiang, Fang, Xu, McCormick, Thomas S, Cooper, Kevin D, Huang, Qiong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5811786/
https://www.ncbi.nlm.nih.gov/pubmed/28295154
http://dx.doi.org/10.1111/ajd.12561
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author Han, Ling
Sugiyama, Hideaki
Zhang, Qi
Yan, Kexiang
Fang, Xu
McCormick, Thomas S
Cooper, Kevin D
Huang, Qiong
author_facet Han, Ling
Sugiyama, Hideaki
Zhang, Qi
Yan, Kexiang
Fang, Xu
McCormick, Thomas S
Cooper, Kevin D
Huang, Qiong
author_sort Han, Ling
collection PubMed
description BACKGROUND: CD39 and CD73 are two novel cell surface markers of CD25(high)Foxp3(+) regulatory T‐cells (Tregs). Concordant expression of these two ectoenzymes not only discriminate Tregs from other cell populations, but also generates pericellular adenosine, which has been reported to suppress proliferation of activated T effector (Teff) cells. Because it is currently unclear whether human ectoenzymes (CD39/CD73) are involved in the impaired suppressive activity of Tregs in psoriasis, we examined the frequencies and phenotypes of CD39/CD73‐expressing Tregs and related receptor adenosine receptor 2A (A(2A) R) in peripheral blood of patients with different types of psoriasis. METHODS: Peripheral blood mononuclear cells (PMBC) were prepared from patients with three different types of psoriasis (psoriasis vulgaris, pustular psoriasis and erythrodermic psoriasis). CD4(+) cells were separated from PBMC by negative selection on midiMACS columns, and the frequencies and phenotypes of CD39 and CD73 expressing Tregs, and A(2A) R expressing Teff were all determined by flow cytometry analysis. Blood from healthy volunteers served as controls. RESULTS: The expression of single CD73(+) Tregs was markedly reduced (approximately 50%) in psoriasis vulgaris, compared to normal controls. In pustular psoriasis, the mean numbers of CD39(+) Tregs and A(2A)R(+) Teff was significantly lower than in normal controls. Among three different types of psoriasis, CD39 expression was strikingly reduced in the blood Treg population of pustular psoriasis patients. Decreased CD73(+) Tregs levels were observed in psoriasis vulgaris compared to pustular psoriasis and erythrodermic psoriasis. CONCLUSIONS: The differences in the expression of CD39(−) and CD73(−) Tregs may be a factor in the pathogenesis of psoriasis.
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spelling pubmed-58117862018-02-16 Phenotypical analysis of ectoenzymes CD39/CD73 and adenosine receptor 2A in CD4(+) CD25(high)Foxp3(+) regulatory T‐cells in psoriasis Han, Ling Sugiyama, Hideaki Zhang, Qi Yan, Kexiang Fang, Xu McCormick, Thomas S Cooper, Kevin D Huang, Qiong Australas J Dermatol Original Research BACKGROUND: CD39 and CD73 are two novel cell surface markers of CD25(high)Foxp3(+) regulatory T‐cells (Tregs). Concordant expression of these two ectoenzymes not only discriminate Tregs from other cell populations, but also generates pericellular adenosine, which has been reported to suppress proliferation of activated T effector (Teff) cells. Because it is currently unclear whether human ectoenzymes (CD39/CD73) are involved in the impaired suppressive activity of Tregs in psoriasis, we examined the frequencies and phenotypes of CD39/CD73‐expressing Tregs and related receptor adenosine receptor 2A (A(2A) R) in peripheral blood of patients with different types of psoriasis. METHODS: Peripheral blood mononuclear cells (PMBC) were prepared from patients with three different types of psoriasis (psoriasis vulgaris, pustular psoriasis and erythrodermic psoriasis). CD4(+) cells were separated from PBMC by negative selection on midiMACS columns, and the frequencies and phenotypes of CD39 and CD73 expressing Tregs, and A(2A) R expressing Teff were all determined by flow cytometry analysis. Blood from healthy volunteers served as controls. RESULTS: The expression of single CD73(+) Tregs was markedly reduced (approximately 50%) in psoriasis vulgaris, compared to normal controls. In pustular psoriasis, the mean numbers of CD39(+) Tregs and A(2A)R(+) Teff was significantly lower than in normal controls. Among three different types of psoriasis, CD39 expression was strikingly reduced in the blood Treg population of pustular psoriasis patients. Decreased CD73(+) Tregs levels were observed in psoriasis vulgaris compared to pustular psoriasis and erythrodermic psoriasis. CONCLUSIONS: The differences in the expression of CD39(−) and CD73(−) Tregs may be a factor in the pathogenesis of psoriasis. John Wiley and Sons Inc. 2017-03-15 2018-02 /pmc/articles/PMC5811786/ /pubmed/28295154 http://dx.doi.org/10.1111/ajd.12561 Text en © 2017 The Authors. Australasian Journal of Dermatology published by Wiley Publishing Asia Pty Ltd on behalf of The Australasian College of Dermatologists. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs (http://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Research
Han, Ling
Sugiyama, Hideaki
Zhang, Qi
Yan, Kexiang
Fang, Xu
McCormick, Thomas S
Cooper, Kevin D
Huang, Qiong
Phenotypical analysis of ectoenzymes CD39/CD73 and adenosine receptor 2A in CD4(+) CD25(high)Foxp3(+) regulatory T‐cells in psoriasis
title Phenotypical analysis of ectoenzymes CD39/CD73 and adenosine receptor 2A in CD4(+) CD25(high)Foxp3(+) regulatory T‐cells in psoriasis
title_full Phenotypical analysis of ectoenzymes CD39/CD73 and adenosine receptor 2A in CD4(+) CD25(high)Foxp3(+) regulatory T‐cells in psoriasis
title_fullStr Phenotypical analysis of ectoenzymes CD39/CD73 and adenosine receptor 2A in CD4(+) CD25(high)Foxp3(+) regulatory T‐cells in psoriasis
title_full_unstemmed Phenotypical analysis of ectoenzymes CD39/CD73 and adenosine receptor 2A in CD4(+) CD25(high)Foxp3(+) regulatory T‐cells in psoriasis
title_short Phenotypical analysis of ectoenzymes CD39/CD73 and adenosine receptor 2A in CD4(+) CD25(high)Foxp3(+) regulatory T‐cells in psoriasis
title_sort phenotypical analysis of ectoenzymes cd39/cd73 and adenosine receptor 2a in cd4(+) cd25(high)foxp3(+) regulatory t‐cells in psoriasis
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5811786/
https://www.ncbi.nlm.nih.gov/pubmed/28295154
http://dx.doi.org/10.1111/ajd.12561
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