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Mitochondria‐targeted antioxidant MitoQ reduced renal damage caused by ischemia‐reperfusion injury in rodent kidneys: Longitudinal observations of T (2)‐weighted imaging and dynamic contrast‐enhanced MRI
PURPOSE: To investigate the effect of mitochondria‐targeted antioxidant MitoQ in reducing the severity of renal ischemia‐reperfusion injury (IRI) in rats using T(2)‐weighted imaging and dynamic contrast‐enhanced MRI (DCE‐MRI). METHODS: Ischemia‐reperfusion injury was induced by temporarily clamping...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5811825/ https://www.ncbi.nlm.nih.gov/pubmed/28608403 http://dx.doi.org/10.1002/mrm.26772 |
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author | Liu, Xiaoge Murphy, Michael P. Xing, Wei Wu, Huanhuan Zhang, Rui Sun, Haoran |
author_facet | Liu, Xiaoge Murphy, Michael P. Xing, Wei Wu, Huanhuan Zhang, Rui Sun, Haoran |
author_sort | Liu, Xiaoge |
collection | PubMed |
description | PURPOSE: To investigate the effect of mitochondria‐targeted antioxidant MitoQ in reducing the severity of renal ischemia‐reperfusion injury (IRI) in rats using T(2)‐weighted imaging and dynamic contrast‐enhanced MRI (DCE‐MRI). METHODS: Ischemia‐reperfusion injury was induced by temporarily clamping the left renal artery. Rats were pretreated with MitoQ or saline. The MRI examination was performed before and after IRI (days 2, 5, 7, and 14). The T(2)‐weighted standardized signal intensity of the outer stripe of the outer medulla (OSOM) was measured. The unilateral renal clearance rate k(cl) was derived from DCE‐MRI. Histopathology was evaluated after the final MRI examination. RESULTS: The standardized signal intensity of the OSOM on IRI kidneys with MitoQ were lower than those with saline on days 5 and 7 (P = 0.004, P < 0.001, respectively). K(cl) values of IRI kidneys with MitoQ were higher than those with saline at all time points (P = 0.002, P < 0.001, P = 0.001, P < 0.001). Histopathology showed that renal damage was the most predominant on the OSOM of IRI kidneys with saline, which was less obvious with MitoQ (P < 0.001). CONCLUSIONS: These findings demonstrate that MitoQ can reduce the severity of renal damage in rodent IRI models using T(2)‐weighted imaging and DCE‐MRI. Magn Reson Med 79:1559–1667, 2018. © 2017 The Authors Magnetic Resonance in Medicine published by Wiley Periodicals, Inc. on behalf of International Society for Magnetic Resonance in Medicine. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
format | Online Article Text |
id | pubmed-5811825 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-58118252018-02-16 Mitochondria‐targeted antioxidant MitoQ reduced renal damage caused by ischemia‐reperfusion injury in rodent kidneys: Longitudinal observations of T (2)‐weighted imaging and dynamic contrast‐enhanced MRI Liu, Xiaoge Murphy, Michael P. Xing, Wei Wu, Huanhuan Zhang, Rui Sun, Haoran Magn Reson Med Full Papers—Preclinical and Clinical Imaging PURPOSE: To investigate the effect of mitochondria‐targeted antioxidant MitoQ in reducing the severity of renal ischemia‐reperfusion injury (IRI) in rats using T(2)‐weighted imaging and dynamic contrast‐enhanced MRI (DCE‐MRI). METHODS: Ischemia‐reperfusion injury was induced by temporarily clamping the left renal artery. Rats were pretreated with MitoQ or saline. The MRI examination was performed before and after IRI (days 2, 5, 7, and 14). The T(2)‐weighted standardized signal intensity of the outer stripe of the outer medulla (OSOM) was measured. The unilateral renal clearance rate k(cl) was derived from DCE‐MRI. Histopathology was evaluated after the final MRI examination. RESULTS: The standardized signal intensity of the OSOM on IRI kidneys with MitoQ were lower than those with saline on days 5 and 7 (P = 0.004, P < 0.001, respectively). K(cl) values of IRI kidneys with MitoQ were higher than those with saline at all time points (P = 0.002, P < 0.001, P = 0.001, P < 0.001). Histopathology showed that renal damage was the most predominant on the OSOM of IRI kidneys with saline, which was less obvious with MitoQ (P < 0.001). CONCLUSIONS: These findings demonstrate that MitoQ can reduce the severity of renal damage in rodent IRI models using T(2)‐weighted imaging and DCE‐MRI. Magn Reson Med 79:1559–1667, 2018. © 2017 The Authors Magnetic Resonance in Medicine published by Wiley Periodicals, Inc. on behalf of International Society for Magnetic Resonance in Medicine. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. John Wiley and Sons Inc. 2017-06-12 2018-03 /pmc/articles/PMC5811825/ /pubmed/28608403 http://dx.doi.org/10.1002/mrm.26772 Text en © 2017 The Authors Magnetic Resonance in Medicine published by Wiley Periodicals, Inc. on behalf of International Society for Magnetic Resonance in Medicine This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Full Papers—Preclinical and Clinical Imaging Liu, Xiaoge Murphy, Michael P. Xing, Wei Wu, Huanhuan Zhang, Rui Sun, Haoran Mitochondria‐targeted antioxidant MitoQ reduced renal damage caused by ischemia‐reperfusion injury in rodent kidneys: Longitudinal observations of T (2)‐weighted imaging and dynamic contrast‐enhanced MRI |
title | Mitochondria‐targeted antioxidant MitoQ reduced renal damage caused by ischemia‐reperfusion injury in rodent kidneys: Longitudinal observations of T
(2)‐weighted imaging and dynamic contrast‐enhanced MRI
|
title_full | Mitochondria‐targeted antioxidant MitoQ reduced renal damage caused by ischemia‐reperfusion injury in rodent kidneys: Longitudinal observations of T
(2)‐weighted imaging and dynamic contrast‐enhanced MRI
|
title_fullStr | Mitochondria‐targeted antioxidant MitoQ reduced renal damage caused by ischemia‐reperfusion injury in rodent kidneys: Longitudinal observations of T
(2)‐weighted imaging and dynamic contrast‐enhanced MRI
|
title_full_unstemmed | Mitochondria‐targeted antioxidant MitoQ reduced renal damage caused by ischemia‐reperfusion injury in rodent kidneys: Longitudinal observations of T
(2)‐weighted imaging and dynamic contrast‐enhanced MRI
|
title_short | Mitochondria‐targeted antioxidant MitoQ reduced renal damage caused by ischemia‐reperfusion injury in rodent kidneys: Longitudinal observations of T
(2)‐weighted imaging and dynamic contrast‐enhanced MRI
|
title_sort | mitochondria‐targeted antioxidant mitoq reduced renal damage caused by ischemia‐reperfusion injury in rodent kidneys: longitudinal observations of t
(2)‐weighted imaging and dynamic contrast‐enhanced mri |
topic | Full Papers—Preclinical and Clinical Imaging |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5811825/ https://www.ncbi.nlm.nih.gov/pubmed/28608403 http://dx.doi.org/10.1002/mrm.26772 |
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