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DNA Damage Protecting Activity and Antioxidant Potential of Launaea taraxacifolia Leaves Extract

BACKGROUND: The leaf extract of Launaea taraxacifolia commonly known as African Lettuce is used locally to treat dyslipidemia and liver diseases, which are associated with oxidative stress. Methanol extract from L. taraxacifolia leaves was tested for its antioxidant activity and its ability to prote...

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Autores principales: Adinortey, Michael Buenor, Ansah, Charles, Weremfo, Alexander, Adinortey, Cynthia Ayefoumi, Adukpo, Genevieve Etornam, Ameyaw, Elvis Ofori, Nyarko, Alexander Kwadwo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5812077/
https://www.ncbi.nlm.nih.gov/pubmed/29456385
http://dx.doi.org/10.4103/jnsbm.JNSBM_22_17
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author Adinortey, Michael Buenor
Ansah, Charles
Weremfo, Alexander
Adinortey, Cynthia Ayefoumi
Adukpo, Genevieve Etornam
Ameyaw, Elvis Ofori
Nyarko, Alexander Kwadwo
author_facet Adinortey, Michael Buenor
Ansah, Charles
Weremfo, Alexander
Adinortey, Cynthia Ayefoumi
Adukpo, Genevieve Etornam
Ameyaw, Elvis Ofori
Nyarko, Alexander Kwadwo
author_sort Adinortey, Michael Buenor
collection PubMed
description BACKGROUND: The leaf extract of Launaea taraxacifolia commonly known as African Lettuce is used locally to treat dyslipidemia and liver diseases, which are associated with oxidative stress. Methanol extract from L. taraxacifolia leaves was tested for its antioxidant activity and its ability to protect DNA from oxidative damage. MATERIALS AND METHODS: In vitro antioxidant potential of the leaf extract was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH), nitric oxide (NO), and hydroxyl (OH) radical scavenging assays. Ferric reducing power, total antioxidant capacity (TAC), metal chelating, and anti-lipid peroxidation ability of the extract were also examined using gallic acid, ascorbic acid, citric acid, and ethylenediaminetetraacetic acid as standards. RESULTS: L. taraxacifolia leaves extract showed antioxidant activity with IC(50) values of 16.18 μg/ml (DPPH), 123.3 μg/ml (NO), 128.2 μg/ml (OH radical), 97.94 μg/ml (metal chelating), 80.28 μg/ml (TAC), and 23 μg/ml (anti-lipid peroxidation activity). L. taraxacifolia leaves extract exhibited a strong capability for DNA damage protection at 20 mg/ml concentration. CONCLUSION: These findings suggest that the methanolic leaf extract of L. taraxacifolia could be used as a natural antioxidant and also as a preventive therapy against diseases such as arteriosclerosis associated with DNA damage.
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spelling pubmed-58120772018-02-16 DNA Damage Protecting Activity and Antioxidant Potential of Launaea taraxacifolia Leaves Extract Adinortey, Michael Buenor Ansah, Charles Weremfo, Alexander Adinortey, Cynthia Ayefoumi Adukpo, Genevieve Etornam Ameyaw, Elvis Ofori Nyarko, Alexander Kwadwo J Nat Sci Biol Med Original Article BACKGROUND: The leaf extract of Launaea taraxacifolia commonly known as African Lettuce is used locally to treat dyslipidemia and liver diseases, which are associated with oxidative stress. Methanol extract from L. taraxacifolia leaves was tested for its antioxidant activity and its ability to protect DNA from oxidative damage. MATERIALS AND METHODS: In vitro antioxidant potential of the leaf extract was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH), nitric oxide (NO), and hydroxyl (OH) radical scavenging assays. Ferric reducing power, total antioxidant capacity (TAC), metal chelating, and anti-lipid peroxidation ability of the extract were also examined using gallic acid, ascorbic acid, citric acid, and ethylenediaminetetraacetic acid as standards. RESULTS: L. taraxacifolia leaves extract showed antioxidant activity with IC(50) values of 16.18 μg/ml (DPPH), 123.3 μg/ml (NO), 128.2 μg/ml (OH radical), 97.94 μg/ml (metal chelating), 80.28 μg/ml (TAC), and 23 μg/ml (anti-lipid peroxidation activity). L. taraxacifolia leaves extract exhibited a strong capability for DNA damage protection at 20 mg/ml concentration. CONCLUSION: These findings suggest that the methanolic leaf extract of L. taraxacifolia could be used as a natural antioxidant and also as a preventive therapy against diseases such as arteriosclerosis associated with DNA damage. Medknow Publications & Media Pvt Ltd 2018 /pmc/articles/PMC5812077/ /pubmed/29456385 http://dx.doi.org/10.4103/jnsbm.JNSBM_22_17 Text en Copyright: © 2018 Journal of Natural Science, Biology and Medicine http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Adinortey, Michael Buenor
Ansah, Charles
Weremfo, Alexander
Adinortey, Cynthia Ayefoumi
Adukpo, Genevieve Etornam
Ameyaw, Elvis Ofori
Nyarko, Alexander Kwadwo
DNA Damage Protecting Activity and Antioxidant Potential of Launaea taraxacifolia Leaves Extract
title DNA Damage Protecting Activity and Antioxidant Potential of Launaea taraxacifolia Leaves Extract
title_full DNA Damage Protecting Activity and Antioxidant Potential of Launaea taraxacifolia Leaves Extract
title_fullStr DNA Damage Protecting Activity and Antioxidant Potential of Launaea taraxacifolia Leaves Extract
title_full_unstemmed DNA Damage Protecting Activity and Antioxidant Potential of Launaea taraxacifolia Leaves Extract
title_short DNA Damage Protecting Activity and Antioxidant Potential of Launaea taraxacifolia Leaves Extract
title_sort dna damage protecting activity and antioxidant potential of launaea taraxacifolia leaves extract
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5812077/
https://www.ncbi.nlm.nih.gov/pubmed/29456385
http://dx.doi.org/10.4103/jnsbm.JNSBM_22_17
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