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Flow rate dependent continuous hydrolysis of protein isolates
Food protein hydrolysates are often produced in unspecific industrial batch processes. The hydrolysates composition underlies process-related fluctuations and therefore the obtained peptide fingerprint and bioactive properties may vary. To overcome this obstacle and enable the production of specific...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5812119/ https://www.ncbi.nlm.nih.gov/pubmed/29429128 http://dx.doi.org/10.1186/s13568-018-0548-9 |
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author | Sewczyk, Tim Hoog Antink, Marieke Maas, Michael Kroll, Stephen Beutel, Sascha |
author_facet | Sewczyk, Tim Hoog Antink, Marieke Maas, Michael Kroll, Stephen Beutel, Sascha |
author_sort | Sewczyk, Tim |
collection | PubMed |
description | Food protein hydrolysates are often produced in unspecific industrial batch processes. The hydrolysates composition underlies process-related fluctuations and therefore the obtained peptide fingerprint and bioactive properties may vary. To overcome this obstacle and enable the production of specific hydrolysates with selected peptides, a ceramic capillary system was developed and characterized for the continuous production of a consistent peptide composition. Therefore, the protease Alcalase was immobilized on the surface of aminosilane modified yttria stabilized zirconia capillaries with a pore size of 1.5 µm. The loading capacity was 0.3 µg enzyme per mg of capillary with a residual enzyme activity of 43%. The enzyme specific peptide fingerprint produced with this proteolytic capillary reactor system correlated with the degree of hydrolysis, which can be controlled over the residence time by adjusting the flow rate. Common food proteins like casein, sunflower and lupin protein isolates were tested for continuous hydrolysis in the developed reactor system. The peptide formation was investigated by high-performance liquid chromatography. Various trends were found for the occurrence of specific peptides. Some are just intermediately occurring, while others cumulate by time. Thus, the developed continuous reactor system enables the production of specific peptides with desired bioactive properties. |
format | Online Article Text |
id | pubmed-5812119 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-58121192018-02-26 Flow rate dependent continuous hydrolysis of protein isolates Sewczyk, Tim Hoog Antink, Marieke Maas, Michael Kroll, Stephen Beutel, Sascha AMB Express Original Article Food protein hydrolysates are often produced in unspecific industrial batch processes. The hydrolysates composition underlies process-related fluctuations and therefore the obtained peptide fingerprint and bioactive properties may vary. To overcome this obstacle and enable the production of specific hydrolysates with selected peptides, a ceramic capillary system was developed and characterized for the continuous production of a consistent peptide composition. Therefore, the protease Alcalase was immobilized on the surface of aminosilane modified yttria stabilized zirconia capillaries with a pore size of 1.5 µm. The loading capacity was 0.3 µg enzyme per mg of capillary with a residual enzyme activity of 43%. The enzyme specific peptide fingerprint produced with this proteolytic capillary reactor system correlated with the degree of hydrolysis, which can be controlled over the residence time by adjusting the flow rate. Common food proteins like casein, sunflower and lupin protein isolates were tested for continuous hydrolysis in the developed reactor system. The peptide formation was investigated by high-performance liquid chromatography. Various trends were found for the occurrence of specific peptides. Some are just intermediately occurring, while others cumulate by time. Thus, the developed continuous reactor system enables the production of specific peptides with desired bioactive properties. Springer Berlin Heidelberg 2018-02-10 /pmc/articles/PMC5812119/ /pubmed/29429128 http://dx.doi.org/10.1186/s13568-018-0548-9 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Article Sewczyk, Tim Hoog Antink, Marieke Maas, Michael Kroll, Stephen Beutel, Sascha Flow rate dependent continuous hydrolysis of protein isolates |
title | Flow rate dependent continuous hydrolysis of protein isolates |
title_full | Flow rate dependent continuous hydrolysis of protein isolates |
title_fullStr | Flow rate dependent continuous hydrolysis of protein isolates |
title_full_unstemmed | Flow rate dependent continuous hydrolysis of protein isolates |
title_short | Flow rate dependent continuous hydrolysis of protein isolates |
title_sort | flow rate dependent continuous hydrolysis of protein isolates |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5812119/ https://www.ncbi.nlm.nih.gov/pubmed/29429128 http://dx.doi.org/10.1186/s13568-018-0548-9 |
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