Cargando…
The altered expression of perineuronal net elements during neural differentiation
BACKGROUND: Perineuronal nets (PNNs), which are localized around neurons during development, are specialized forms of neural extracellular matrix with neuroprotective and plasticity-regulating roles. Hyaluronan and proteoglycan link protein 1 (HAPLN1), tenascin-R (TNR) and aggrecan (ACAN) are key el...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5812217/ https://www.ncbi.nlm.nih.gov/pubmed/29456557 http://dx.doi.org/10.1186/s11658-018-0073-5 |
_version_ | 1783300005722849280 |
---|---|
author | Eskici, Nazli F. Erdem-Ozdamar, Sevim Dayangac-Erden, Didem |
author_facet | Eskici, Nazli F. Erdem-Ozdamar, Sevim Dayangac-Erden, Didem |
author_sort | Eskici, Nazli F. |
collection | PubMed |
description | BACKGROUND: Perineuronal nets (PNNs), which are localized around neurons during development, are specialized forms of neural extracellular matrix with neuroprotective and plasticity-regulating roles. Hyaluronan and proteoglycan link protein 1 (HAPLN1), tenascin-R (TNR) and aggrecan (ACAN) are key elements of PNNs. In diseases characterized by neuritogenesis defects, the expression of these proteins is known to be downregulated, suggesting that PNNs may have a role in neural differentiation. METHODS: In this study, the mRNA and protein levels of HAPLN1, TNR and ACAN were determined and compared at specific time points of neural differentiation. We used PC12 cells as the in vitro model because they reflect this developmental process. RESULTS: On day 7, the HAPLN1 mRNA level showed a 2.9-fold increase compared to the non-differentiated state. However, the cellular HAPLN1 protein level showed a decrease, indicating that the protein may have roles in neural differentiation, and may be secreted during the early period of differentiation. By contrast, TNR mRNA and protein levels remained unchanged, and the amount of cellular ACAN protein showed a 3.7-fold increase at day 7. These results suggest that ACAN may be secreted after day 7, possibly due to its large amount of post-translational modifications. CONCLUSIONS: Our results provide preliminary data on the expression of PNN elements during neural differentiation. Further investigations will be performed on the role of these elements in neurological disease models. |
format | Online Article Text |
id | pubmed-5812217 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-58122172018-02-16 The altered expression of perineuronal net elements during neural differentiation Eskici, Nazli F. Erdem-Ozdamar, Sevim Dayangac-Erden, Didem Cell Mol Biol Lett Research BACKGROUND: Perineuronal nets (PNNs), which are localized around neurons during development, are specialized forms of neural extracellular matrix with neuroprotective and plasticity-regulating roles. Hyaluronan and proteoglycan link protein 1 (HAPLN1), tenascin-R (TNR) and aggrecan (ACAN) are key elements of PNNs. In diseases characterized by neuritogenesis defects, the expression of these proteins is known to be downregulated, suggesting that PNNs may have a role in neural differentiation. METHODS: In this study, the mRNA and protein levels of HAPLN1, TNR and ACAN were determined and compared at specific time points of neural differentiation. We used PC12 cells as the in vitro model because they reflect this developmental process. RESULTS: On day 7, the HAPLN1 mRNA level showed a 2.9-fold increase compared to the non-differentiated state. However, the cellular HAPLN1 protein level showed a decrease, indicating that the protein may have roles in neural differentiation, and may be secreted during the early period of differentiation. By contrast, TNR mRNA and protein levels remained unchanged, and the amount of cellular ACAN protein showed a 3.7-fold increase at day 7. These results suggest that ACAN may be secreted after day 7, possibly due to its large amount of post-translational modifications. CONCLUSIONS: Our results provide preliminary data on the expression of PNN elements during neural differentiation. Further investigations will be performed on the role of these elements in neurological disease models. BioMed Central 2018-02-13 /pmc/articles/PMC5812217/ /pubmed/29456557 http://dx.doi.org/10.1186/s11658-018-0073-5 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Eskici, Nazli F. Erdem-Ozdamar, Sevim Dayangac-Erden, Didem The altered expression of perineuronal net elements during neural differentiation |
title | The altered expression of perineuronal net elements during neural differentiation |
title_full | The altered expression of perineuronal net elements during neural differentiation |
title_fullStr | The altered expression of perineuronal net elements during neural differentiation |
title_full_unstemmed | The altered expression of perineuronal net elements during neural differentiation |
title_short | The altered expression of perineuronal net elements during neural differentiation |
title_sort | altered expression of perineuronal net elements during neural differentiation |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5812217/ https://www.ncbi.nlm.nih.gov/pubmed/29456557 http://dx.doi.org/10.1186/s11658-018-0073-5 |
work_keys_str_mv | AT eskicinazlif thealteredexpressionofperineuronalnetelementsduringneuraldifferentiation AT erdemozdamarsevim thealteredexpressionofperineuronalnetelementsduringneuraldifferentiation AT dayangacerdendidem thealteredexpressionofperineuronalnetelementsduringneuraldifferentiation AT eskicinazlif alteredexpressionofperineuronalnetelementsduringneuraldifferentiation AT erdemozdamarsevim alteredexpressionofperineuronalnetelementsduringneuraldifferentiation AT dayangacerdendidem alteredexpressionofperineuronalnetelementsduringneuraldifferentiation |