1α,25-dihydroxyvitamin D(3) mitigates cancer cell mediated mitochondrial dysfunction in human skeletal muscle cells

Cancer cachexia is associated with muscle weakness and atrophy. We investigated whether 1α,25-dihydroxyvitamin D(3) (1α,25(OH)(2)D(3)), which has previously been shown to increase skeletal myoblast oxygen consumption rate, could reverse the deleterious effects of tumor cell conditioned medium on myo...

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Autores principales: Ryan, Zachary C., Craig, Theodore A., Wang, Xuewei, Delmotte, Philippe, Salisbury, Jeffrey L., Lanza, Ian R., Sieck, Gary C., Kumar, Rajiv
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5812288/
https://www.ncbi.nlm.nih.gov/pubmed/29366785
http://dx.doi.org/10.1016/j.bbrc.2018.01.092
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author Ryan, Zachary C.
Craig, Theodore A.
Wang, Xuewei
Delmotte, Philippe
Salisbury, Jeffrey L.
Lanza, Ian R.
Sieck, Gary C.
Kumar, Rajiv
author_facet Ryan, Zachary C.
Craig, Theodore A.
Wang, Xuewei
Delmotte, Philippe
Salisbury, Jeffrey L.
Lanza, Ian R.
Sieck, Gary C.
Kumar, Rajiv
author_sort Ryan, Zachary C.
collection PubMed
description Cancer cachexia is associated with muscle weakness and atrophy. We investigated whether 1α,25-dihydroxyvitamin D(3) (1α,25(OH)(2)D(3)), which has previously been shown to increase skeletal myoblast oxygen consumption rate, could reverse the deleterious effects of tumor cell conditioned medium on myoblast function. Conditioned medium from Lewis lung carcinoma (LLC1) cells inhibits oxygen consumption, increases mitochondrial fragmentation, inhibits pyruvate dehydrogenase activity, and enhances proteasomal activity in human skeletal muscle myoblasts. 1α,25(OH)(2)D(3) reverses the tumor cell-mediated changes in mitochondrial oxygen consumption and proteasomal activity, without changing pyruvate dehydrogenase activity. 1α,25(OH)(2)D(3) might be useful in treatment of weakness seen in association with CC.
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spelling pubmed-58122882018-02-14 1α,25-dihydroxyvitamin D(3) mitigates cancer cell mediated mitochondrial dysfunction in human skeletal muscle cells Ryan, Zachary C. Craig, Theodore A. Wang, Xuewei Delmotte, Philippe Salisbury, Jeffrey L. Lanza, Ian R. Sieck, Gary C. Kumar, Rajiv Biochem Biophys Res Commun Article Cancer cachexia is associated with muscle weakness and atrophy. We investigated whether 1α,25-dihydroxyvitamin D(3) (1α,25(OH)(2)D(3)), which has previously been shown to increase skeletal myoblast oxygen consumption rate, could reverse the deleterious effects of tumor cell conditioned medium on myoblast function. Conditioned medium from Lewis lung carcinoma (LLC1) cells inhibits oxygen consumption, increases mitochondrial fragmentation, inhibits pyruvate dehydrogenase activity, and enhances proteasomal activity in human skeletal muscle myoblasts. 1α,25(OH)(2)D(3) reverses the tumor cell-mediated changes in mitochondrial oxygen consumption and proteasomal activity, without changing pyruvate dehydrogenase activity. 1α,25(OH)(2)D(3) might be useful in treatment of weakness seen in association with CC. 2018-02-05 /pmc/articles/PMC5812288/ /pubmed/29366785 http://dx.doi.org/10.1016/j.bbrc.2018.01.092 Text en http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Ryan, Zachary C.
Craig, Theodore A.
Wang, Xuewei
Delmotte, Philippe
Salisbury, Jeffrey L.
Lanza, Ian R.
Sieck, Gary C.
Kumar, Rajiv
1α,25-dihydroxyvitamin D(3) mitigates cancer cell mediated mitochondrial dysfunction in human skeletal muscle cells
title 1α,25-dihydroxyvitamin D(3) mitigates cancer cell mediated mitochondrial dysfunction in human skeletal muscle cells
title_full 1α,25-dihydroxyvitamin D(3) mitigates cancer cell mediated mitochondrial dysfunction in human skeletal muscle cells
title_fullStr 1α,25-dihydroxyvitamin D(3) mitigates cancer cell mediated mitochondrial dysfunction in human skeletal muscle cells
title_full_unstemmed 1α,25-dihydroxyvitamin D(3) mitigates cancer cell mediated mitochondrial dysfunction in human skeletal muscle cells
title_short 1α,25-dihydroxyvitamin D(3) mitigates cancer cell mediated mitochondrial dysfunction in human skeletal muscle cells
title_sort 1α,25-dihydroxyvitamin d(3) mitigates cancer cell mediated mitochondrial dysfunction in human skeletal muscle cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5812288/
https://www.ncbi.nlm.nih.gov/pubmed/29366785
http://dx.doi.org/10.1016/j.bbrc.2018.01.092
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