Cargando…

Determination of diffusion coefficients in live cells using fluorescence recovery after photobleaching with wide-field fluorescence microscopy

Fluorescence recovery after photobleaching (FRAP) enables characterization of quantitative dynamic properties such as diffusion coefficients of fluorescent molecules in living cells by analyzing the recovery of fluorescence intensity after photobleaching in a specific cellular compartment or area. T...

Descripción completa

Detalles Bibliográficos
Autores principales:	Kitamura, Akira, Kinjo, Masataka
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	The Biophysical Society of Japan (BSJ) 2018
Materias:	Regular Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5812315/ https://www.ncbi.nlm.nih.gov/pubmed/29450109 http://dx.doi.org/10.2142/biophysico.15.0_1

Ejemplares similares

Photobleaching and Fluorescence Recovery of RPE Bisretinoids
por: Liu, Zhao, et al.
Publicado: (2015)

Determining vitreous viscosity using fluorescence recovery after photobleaching
por: Srikantha, Nishanthan, et al.
Publicado: (2022)

Defining the Diffusion in Model Membranes Using Line Fluorescence Recovery after Photobleaching
por: Kure, Jakob L., et al.
Publicado: (2020)

Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching
por: Hildick, Keri L., et al.
Publicado: (2012)

Resonant Scanning with Large Field of View Reduces Photobleaching and Enhances Fluorescence Yield in STED Microscopy
por: Wu, Yong, et al.
Publicado: (2015)

Parameter estimation in fluorescence recovery after photobleaching: quantitative analysis of protein binding reactions and diffusion
por: Williamson, Daniel E., et al.
Publicado: (2021)

Numerical Simulation of the Photobleaching Process in Laser-Induced Fluorescence Photobleaching Anemometer
por: Chen, Yu, et al.
Publicado: (2021)

Using Fluorescence Recovery After Photobleaching data to uncover filament dynamics
por: Dallon, J. C., et al.
Publicado: (2022)

Monitoring condensate dynamics in S. cerevisiae using fluorescence recovery after photobleaching
por: Sprunger, Macy L., et al.
Publicado: (2022)

The Utility of Fluorescence Recovery after Photobleaching (FRAP) to Study the Plasma Membrane
por: Day, Charles A., et al.
Publicado: (2023)

Protocol for single-molecule fluorescence recovery after photobleaching microscopy to analyze the dynamics and spatial locations of nuclear transmembrane proteins in live cells
por: Tingey, Mark, et al.
Publicado: (2021)

Fluorescence Recovery After Photobleaching (FRAP) of Fluorescence Tagged Proteins in Dendritic Spines of Cultured Hippocampal Neurons
por: Zheng, Chan-Ying, et al.
Publicado: (2011)

Do photobleached fluorescent microtubules move?: re-evaluation of fluorescence laser photobleaching both in vitro and in growing Xenopus axon
Publicado: (1993)

Age-dependency of molecular diffusion in the human anterior lens capsule assessed using fluorescence recovery after photobleaching
por: Sueiras, Vivian M., et al.
Publicado: (2019)

Recent Applications of Fluorescence Recovery after Photobleaching (FRAP) to Membrane Bio-Macromolecules
por: Rayan, Gamal, et al.
Publicado: (2010)

Assessing cellular efficacy of bromodomain inhibitors using fluorescence recovery after photobleaching
por: Philpott, Martin, et al.
Publicado: (2014)

Digital imaging fluorescence microscopy: spatial heterogeneity of photobleaching rate constants in individual cells
Publicado: (1985)

Diffusion coefficient of fluorescein-labeled tubulin in the cytoplasm of embryonic cells of a sea urchin: video image analysis of fluorescence redistribution after photobleaching
Publicado: (1984)

State-of-the-Art Fluorescence Fluctuation-Based Spectroscopic Techniques for the Study of Protein Aggregation
por: Kitamura, Akira, et al.
Publicado: (2018)

EGF Receptor Stalls upon Activation as Evidenced by Complementary Fluorescence Correlation Spectroscopy and Fluorescence Recovery after Photobleaching Measurements
por: Vámosi, György, et al.
Publicado: (2019)

A Discontinuous Galerkin Model for Fluorescence Loss in Photobleaching
por: Hansen, Christian V., et al.
Publicado: (2018)

A Combined Acceptor Photobleaching and Donor Fluorescence Lifetime Imaging Microscopy Approach to Analyze Multi-Protein Interactions in Living Cells
por: Eckenstaler, Robert, et al.
Publicado: (2021)

Dynamic Mode Decomposition of Fluorescence Loss in Photobleaching Microscopy Data for Model-Free Analysis of Protein Transport and Aggregation in Living Cells
por: Wüstner, Daniel
Publicado: (2022)

A fluorescence recovery after photobleaching protocol to measure surface diffusion of DAGLα in primary cultured cortical mouse neurons
por: Yoon, Sehyoun, et al.
Publicado: (2022)

Probing the segregation of evoked and spontaneous neurotransmission via photobleaching and recovery of a fluorescent glutamate sensor
por: Wang, Camille S, et al.
Publicado: (2022)

Recent Advances in Fluorescence Recovery after Photobleaching for Decoupling Transport and Kinetics of Biomacromolecules in Cellular Physiology
por: Cai, Ning, et al.
Publicado: (2022)

Expanding the applicability of multiphoton fluorescence recovery after photobleaching by incorporating shear stress in laminar flow
por: Elias, Tresa M., et al.
Publicado: (2023)

Fluorescence Photobleaching as an Intrinsic Tool to Quantify the 3D Expansion Factor of Biological Samples in Expansion Microscopy
por: Vanheusden, Marisa, et al.
Publicado: (2020)

Image segmentation and separation of spectrally similar dyes in fluorescence microscopy by dynamic mode decomposition of photobleaching kinetics
por: Wüstner, Daniel
Publicado: (2022)

Quantitative fluorescence loss in photobleaching for analysis of protein transport and aggregation
por: Wüstner, Daniel, et al.
Publicado: (2012)

EasyFRAP-web: a web-based tool for the analysis of fluorescence recovery after photobleaching data
por: Koulouras, Grigorios, et al.
Publicado: (2018)

Investigating the effect of proteoglycan 4 on hyaluronan solution properties using confocal fluorescence recovery after photobleaching
por: Bloom, Adam K., et al.
Publicado: (2019)

Enzyme-dependent fluorescence recovery of NADH after photobleaching to assess dehydrogenase activity of isolated perfused hearts
por: Moreno, Angel, et al.
Publicado: (2017)

DeepFRAP: Fast fluorescence recovery after photobleaching data analysis using deep neural networks
por: Wåhlstrand Skärström, Victor, et al.
Publicado: (2021)

Nasal epithelial cell culture fluorescence recovery after photobleaching predicts cystic fibrosis therapeutic response
por: Corcoran, Timothy E., et al.
Publicado: (2022)

Chemical factors that influence nucleocytoplasmic transport: a fluorescence photobleaching study
Publicado: (1986)

Photobleaching of YOYO-1 in super-resolution single DNA fluorescence imaging
por: Pyle, Joseph R, et al.
Publicado: (2017)

Photobleaching Kinetics and Time-Integrated Emission of Fluorescent Probes in Cellular Membranes
por: Wüstner, Daniel, et al.
Publicado: (2014)

Wide-Field Fluorescence Microscopy of Real-Time Bioconjugation Sensing
por: Szalkowski, Marcin, et al.
Publicado: (2018)

Single-Molecule Fluorescence Microscopy Reveals Local Diffusion Coefficients in the Pore Network of an Individual Catalyst Particle
por: Hendriks, Frank C., et al.
Publicado: (2017)

Cannot write session to /tmp/vufind_sessions/sess_5l8rp4tl3f7jl4vt18k5d097i9