Cargando…
Development and characterization of two cell lines from gills of Atlantic salmon
Gill disease in Atlantic salmon, Salmo salar L., causes big losses in the salmon farming industry. Until now, tools to cultivate microorganisms causing gill disease and models to study the gill responses have been lacking. Here we describe the establishment and characterization of two cell lines fro...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5812586/ https://www.ncbi.nlm.nih.gov/pubmed/29444101 http://dx.doi.org/10.1371/journal.pone.0191792 |
_version_ | 1783300053039841280 |
---|---|
author | Gjessing, Mona C. Aamelfot, Maria Batts, William N. Benestad, Sylvie L. Dale, Ole B. Thoen, Even Weli, Simon C. Winton, James R. |
author_facet | Gjessing, Mona C. Aamelfot, Maria Batts, William N. Benestad, Sylvie L. Dale, Ole B. Thoen, Even Weli, Simon C. Winton, James R. |
author_sort | Gjessing, Mona C. |
collection | PubMed |
description | Gill disease in Atlantic salmon, Salmo salar L., causes big losses in the salmon farming industry. Until now, tools to cultivate microorganisms causing gill disease and models to study the gill responses have been lacking. Here we describe the establishment and characterization of two cell lines from the gills of Atlantic salmon. Atlantic salmon gill cell ASG-10 consisted of cells staining for cytokeratin and e-cadherin and with desmosomes as seen by transmission electron microscopy suggesting the cells to be of epithelial origin. These structures were not seen in ASG-13. The cell lines have been maintained for almost 30 passages and both cell lines are fully susceptible to infection by infectious hematopoietic necrosis virus (IHNV), viral hemorrhagic septicemia virus (VHSV), infectious pancreatic necrosis virus (IPNV), Atlantic salmon reovirus TS (TSRV) and Pacific salmon paramyxovirus (PSPV). While infectious salmon anemia virus (ISAV) did not cause visible CPE, immunofluorescent staining revealed a sub-fraction of cells in both the ASG-10 and ASG-13 lines may be permissive to infection. ASG-10 is able to proliferate and migrate to close scratches in the monolayer within seven days in vitro contrary to ASG-13, which does not appear to do have the same proliferative and migratory ability. These cell lines will be useful in studies of gill diseases in Atlantic salmon and may represent an important contribution for alternatives to experimental animals and studies of epithelial–mesenchymal cell biology. |
format | Online Article Text |
id | pubmed-5812586 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-58125862018-02-28 Development and characterization of two cell lines from gills of Atlantic salmon Gjessing, Mona C. Aamelfot, Maria Batts, William N. Benestad, Sylvie L. Dale, Ole B. Thoen, Even Weli, Simon C. Winton, James R. PLoS One Research Article Gill disease in Atlantic salmon, Salmo salar L., causes big losses in the salmon farming industry. Until now, tools to cultivate microorganisms causing gill disease and models to study the gill responses have been lacking. Here we describe the establishment and characterization of two cell lines from the gills of Atlantic salmon. Atlantic salmon gill cell ASG-10 consisted of cells staining for cytokeratin and e-cadherin and with desmosomes as seen by transmission electron microscopy suggesting the cells to be of epithelial origin. These structures were not seen in ASG-13. The cell lines have been maintained for almost 30 passages and both cell lines are fully susceptible to infection by infectious hematopoietic necrosis virus (IHNV), viral hemorrhagic septicemia virus (VHSV), infectious pancreatic necrosis virus (IPNV), Atlantic salmon reovirus TS (TSRV) and Pacific salmon paramyxovirus (PSPV). While infectious salmon anemia virus (ISAV) did not cause visible CPE, immunofluorescent staining revealed a sub-fraction of cells in both the ASG-10 and ASG-13 lines may be permissive to infection. ASG-10 is able to proliferate and migrate to close scratches in the monolayer within seven days in vitro contrary to ASG-13, which does not appear to do have the same proliferative and migratory ability. These cell lines will be useful in studies of gill diseases in Atlantic salmon and may represent an important contribution for alternatives to experimental animals and studies of epithelial–mesenchymal cell biology. Public Library of Science 2018-02-14 /pmc/articles/PMC5812586/ /pubmed/29444101 http://dx.doi.org/10.1371/journal.pone.0191792 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication. |
spellingShingle | Research Article Gjessing, Mona C. Aamelfot, Maria Batts, William N. Benestad, Sylvie L. Dale, Ole B. Thoen, Even Weli, Simon C. Winton, James R. Development and characterization of two cell lines from gills of Atlantic salmon |
title | Development and characterization of two cell lines from gills of Atlantic salmon |
title_full | Development and characterization of two cell lines from gills of Atlantic salmon |
title_fullStr | Development and characterization of two cell lines from gills of Atlantic salmon |
title_full_unstemmed | Development and characterization of two cell lines from gills of Atlantic salmon |
title_short | Development and characterization of two cell lines from gills of Atlantic salmon |
title_sort | development and characterization of two cell lines from gills of atlantic salmon |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5812586/ https://www.ncbi.nlm.nih.gov/pubmed/29444101 http://dx.doi.org/10.1371/journal.pone.0191792 |
work_keys_str_mv | AT gjessingmonac developmentandcharacterizationoftwocelllinesfromgillsofatlanticsalmon AT aamelfotmaria developmentandcharacterizationoftwocelllinesfromgillsofatlanticsalmon AT battswilliamn developmentandcharacterizationoftwocelllinesfromgillsofatlanticsalmon AT benestadsylviel developmentandcharacterizationoftwocelllinesfromgillsofatlanticsalmon AT daleoleb developmentandcharacterizationoftwocelllinesfromgillsofatlanticsalmon AT thoeneven developmentandcharacterizationoftwocelllinesfromgillsofatlanticsalmon AT welisimonc developmentandcharacterizationoftwocelllinesfromgillsofatlanticsalmon AT wintonjamesr developmentandcharacterizationoftwocelllinesfromgillsofatlanticsalmon |