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Chemical hijacking of auxin signaling with an engineered auxin-TIR1 pair

The phytohormone auxin, indole-3-acetic acid (IAA), regulates nearly all aspects of plant growth and development. Despite substantial progress in our understanding of auxin biology, delineating specific auxin response remains as a major challenge. Auxin regulates transcriptional response via its rec...

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Autores principales: Uchida, Naoyuki, Takahashi, Koji, Iwasaki, Rie, Yamada, Ryotaro, Yoshimura, Masahiko, Endo, Takaho A., Kimura, Seisuke, Zhang, Hua, Nomoto, Mika, Tada, Yasuomi, Kinoshita, Toshinori, Itami, Kenichiro, Hagihara, Shinya, Torii, Keiko U.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5812785/
https://www.ncbi.nlm.nih.gov/pubmed/29355850
http://dx.doi.org/10.1038/nchembio.2555
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author Uchida, Naoyuki
Takahashi, Koji
Iwasaki, Rie
Yamada, Ryotaro
Yoshimura, Masahiko
Endo, Takaho A.
Kimura, Seisuke
Zhang, Hua
Nomoto, Mika
Tada, Yasuomi
Kinoshita, Toshinori
Itami, Kenichiro
Hagihara, Shinya
Torii, Keiko U.
author_facet Uchida, Naoyuki
Takahashi, Koji
Iwasaki, Rie
Yamada, Ryotaro
Yoshimura, Masahiko
Endo, Takaho A.
Kimura, Seisuke
Zhang, Hua
Nomoto, Mika
Tada, Yasuomi
Kinoshita, Toshinori
Itami, Kenichiro
Hagihara, Shinya
Torii, Keiko U.
author_sort Uchida, Naoyuki
collection PubMed
description The phytohormone auxin, indole-3-acetic acid (IAA), regulates nearly all aspects of plant growth and development. Despite substantial progress in our understanding of auxin biology, delineating specific auxin response remains as a major challenge. Auxin regulates transcriptional response via its receptors, TIR1/AFB F-box proteins. Here we report an engineered, orthogonal auxin-TIR1 receptor pair, developed through a bump-and-hole strategy, that triggers auxin signaling without interfering with endogenous auxin or TIR1/AFBs. A synthetic, convex IAA (cvxIAA) hijacked the downstream auxin signaling in vivo both at the transcriptomic level and in specific developmental contexts, only in the presence of a complementary, concave TIR1 (ccvTIR1) receptor. Harnessing the cvxIAA-ccvTIR1 system, we provide conclusive evidence for the role of TIR1-mediated pathway in auxin-induced seedling acid growth. The cvxIAA-ccvTIR1 system serves as a powerful tool for solving outstanding questions in auxin biology and for precise manipulation of auxin-mediated processes as a controllable switch.
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spelling pubmed-58127852018-07-22 Chemical hijacking of auxin signaling with an engineered auxin-TIR1 pair Uchida, Naoyuki Takahashi, Koji Iwasaki, Rie Yamada, Ryotaro Yoshimura, Masahiko Endo, Takaho A. Kimura, Seisuke Zhang, Hua Nomoto, Mika Tada, Yasuomi Kinoshita, Toshinori Itami, Kenichiro Hagihara, Shinya Torii, Keiko U. Nat Chem Biol Article The phytohormone auxin, indole-3-acetic acid (IAA), regulates nearly all aspects of plant growth and development. Despite substantial progress in our understanding of auxin biology, delineating specific auxin response remains as a major challenge. Auxin regulates transcriptional response via its receptors, TIR1/AFB F-box proteins. Here we report an engineered, orthogonal auxin-TIR1 receptor pair, developed through a bump-and-hole strategy, that triggers auxin signaling without interfering with endogenous auxin or TIR1/AFBs. A synthetic, convex IAA (cvxIAA) hijacked the downstream auxin signaling in vivo both at the transcriptomic level and in specific developmental contexts, only in the presence of a complementary, concave TIR1 (ccvTIR1) receptor. Harnessing the cvxIAA-ccvTIR1 system, we provide conclusive evidence for the role of TIR1-mediated pathway in auxin-induced seedling acid growth. The cvxIAA-ccvTIR1 system serves as a powerful tool for solving outstanding questions in auxin biology and for precise manipulation of auxin-mediated processes as a controllable switch. 2018-01-22 2018-03 /pmc/articles/PMC5812785/ /pubmed/29355850 http://dx.doi.org/10.1038/nchembio.2555 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Uchida, Naoyuki
Takahashi, Koji
Iwasaki, Rie
Yamada, Ryotaro
Yoshimura, Masahiko
Endo, Takaho A.
Kimura, Seisuke
Zhang, Hua
Nomoto, Mika
Tada, Yasuomi
Kinoshita, Toshinori
Itami, Kenichiro
Hagihara, Shinya
Torii, Keiko U.
Chemical hijacking of auxin signaling with an engineered auxin-TIR1 pair
title Chemical hijacking of auxin signaling with an engineered auxin-TIR1 pair
title_full Chemical hijacking of auxin signaling with an engineered auxin-TIR1 pair
title_fullStr Chemical hijacking of auxin signaling with an engineered auxin-TIR1 pair
title_full_unstemmed Chemical hijacking of auxin signaling with an engineered auxin-TIR1 pair
title_short Chemical hijacking of auxin signaling with an engineered auxin-TIR1 pair
title_sort chemical hijacking of auxin signaling with an engineered auxin-tir1 pair
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5812785/
https://www.ncbi.nlm.nih.gov/pubmed/29355850
http://dx.doi.org/10.1038/nchembio.2555
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