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Chemical hijacking of auxin signaling with an engineered auxin-TIR1 pair
The phytohormone auxin, indole-3-acetic acid (IAA), regulates nearly all aspects of plant growth and development. Despite substantial progress in our understanding of auxin biology, delineating specific auxin response remains as a major challenge. Auxin regulates transcriptional response via its rec...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5812785/ https://www.ncbi.nlm.nih.gov/pubmed/29355850 http://dx.doi.org/10.1038/nchembio.2555 |
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author | Uchida, Naoyuki Takahashi, Koji Iwasaki, Rie Yamada, Ryotaro Yoshimura, Masahiko Endo, Takaho A. Kimura, Seisuke Zhang, Hua Nomoto, Mika Tada, Yasuomi Kinoshita, Toshinori Itami, Kenichiro Hagihara, Shinya Torii, Keiko U. |
author_facet | Uchida, Naoyuki Takahashi, Koji Iwasaki, Rie Yamada, Ryotaro Yoshimura, Masahiko Endo, Takaho A. Kimura, Seisuke Zhang, Hua Nomoto, Mika Tada, Yasuomi Kinoshita, Toshinori Itami, Kenichiro Hagihara, Shinya Torii, Keiko U. |
author_sort | Uchida, Naoyuki |
collection | PubMed |
description | The phytohormone auxin, indole-3-acetic acid (IAA), regulates nearly all aspects of plant growth and development. Despite substantial progress in our understanding of auxin biology, delineating specific auxin response remains as a major challenge. Auxin regulates transcriptional response via its receptors, TIR1/AFB F-box proteins. Here we report an engineered, orthogonal auxin-TIR1 receptor pair, developed through a bump-and-hole strategy, that triggers auxin signaling without interfering with endogenous auxin or TIR1/AFBs. A synthetic, convex IAA (cvxIAA) hijacked the downstream auxin signaling in vivo both at the transcriptomic level and in specific developmental contexts, only in the presence of a complementary, concave TIR1 (ccvTIR1) receptor. Harnessing the cvxIAA-ccvTIR1 system, we provide conclusive evidence for the role of TIR1-mediated pathway in auxin-induced seedling acid growth. The cvxIAA-ccvTIR1 system serves as a powerful tool for solving outstanding questions in auxin biology and for precise manipulation of auxin-mediated processes as a controllable switch. |
format | Online Article Text |
id | pubmed-5812785 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
record_format | MEDLINE/PubMed |
spelling | pubmed-58127852018-07-22 Chemical hijacking of auxin signaling with an engineered auxin-TIR1 pair Uchida, Naoyuki Takahashi, Koji Iwasaki, Rie Yamada, Ryotaro Yoshimura, Masahiko Endo, Takaho A. Kimura, Seisuke Zhang, Hua Nomoto, Mika Tada, Yasuomi Kinoshita, Toshinori Itami, Kenichiro Hagihara, Shinya Torii, Keiko U. Nat Chem Biol Article The phytohormone auxin, indole-3-acetic acid (IAA), regulates nearly all aspects of plant growth and development. Despite substantial progress in our understanding of auxin biology, delineating specific auxin response remains as a major challenge. Auxin regulates transcriptional response via its receptors, TIR1/AFB F-box proteins. Here we report an engineered, orthogonal auxin-TIR1 receptor pair, developed through a bump-and-hole strategy, that triggers auxin signaling without interfering with endogenous auxin or TIR1/AFBs. A synthetic, convex IAA (cvxIAA) hijacked the downstream auxin signaling in vivo both at the transcriptomic level and in specific developmental contexts, only in the presence of a complementary, concave TIR1 (ccvTIR1) receptor. Harnessing the cvxIAA-ccvTIR1 system, we provide conclusive evidence for the role of TIR1-mediated pathway in auxin-induced seedling acid growth. The cvxIAA-ccvTIR1 system serves as a powerful tool for solving outstanding questions in auxin biology and for precise manipulation of auxin-mediated processes as a controllable switch. 2018-01-22 2018-03 /pmc/articles/PMC5812785/ /pubmed/29355850 http://dx.doi.org/10.1038/nchembio.2555 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Uchida, Naoyuki Takahashi, Koji Iwasaki, Rie Yamada, Ryotaro Yoshimura, Masahiko Endo, Takaho A. Kimura, Seisuke Zhang, Hua Nomoto, Mika Tada, Yasuomi Kinoshita, Toshinori Itami, Kenichiro Hagihara, Shinya Torii, Keiko U. Chemical hijacking of auxin signaling with an engineered auxin-TIR1 pair |
title | Chemical hijacking of auxin signaling with an engineered auxin-TIR1
pair |
title_full | Chemical hijacking of auxin signaling with an engineered auxin-TIR1
pair |
title_fullStr | Chemical hijacking of auxin signaling with an engineered auxin-TIR1
pair |
title_full_unstemmed | Chemical hijacking of auxin signaling with an engineered auxin-TIR1
pair |
title_short | Chemical hijacking of auxin signaling with an engineered auxin-TIR1
pair |
title_sort | chemical hijacking of auxin signaling with an engineered auxin-tir1
pair |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5812785/ https://www.ncbi.nlm.nih.gov/pubmed/29355850 http://dx.doi.org/10.1038/nchembio.2555 |
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