Molecular prevalence and phylogenetic analysis of Theileria annulata and Trypanosoma evansi in cattle in Northern Tunisia

The present study aimed to estimate the molecular prevalence of Theileria annulata and Trypanosoma evansi infection in cattle in Northern Tunisia. A total number of 96 cattle from five farms were evaluated. T. annulata and T. evansi prevalences were 61% [56/66] and 10% [7/13], respectively, at a con...

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Autores principales: Sallemi, Souhir, Rjeibi, Mohamed R., Rouatbi, Mariem, Amairia, Safa, Ben Said, Mourad, Khamassi Khbou, Madiha, Gharbi, Mohamed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Original Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5813107/
https://www.ncbi.nlm.nih.gov/pubmed/29468077
http://dx.doi.org/10.1002/vms3.79
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author Sallemi, Souhir
Rjeibi, Mohamed R.
Rouatbi, Mariem
Amairia, Safa
Ben Said, Mourad
Khamassi Khbou, Madiha
Gharbi, Mohamed
author_facet Sallemi, Souhir
Rjeibi, Mohamed R.
Rouatbi, Mariem
Amairia, Safa
Ben Said, Mourad
Khamassi Khbou, Madiha
Gharbi, Mohamed
author_sort Sallemi, Souhir
collection PubMed
description The present study aimed to estimate the molecular prevalence of Theileria annulata and Trypanosoma evansi infection in cattle in Northern Tunisia. A total number of 96 cattle from five farms were evaluated. T. annulata and T. evansi prevalences were 61% [56/66] and 10% [7/13], respectively, at a confidence interval (CI) of 95%, while co‐infection was present in 6% [4/8] of the tested animals at a CI of 95%. There was a significant correlation between age and the prevalence of T. annulata infection, whereas, there was no significant association shown with the age of cattle and T. evansi infection. Sequence and phylogenetic analyses showed that the T. annulata Tams1 gene and T. evansi ITS1 rDNA gene were highly conserved with 97.1–100% and 98.3–100% sequence identity, respectively.
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spelling pubmed-58131072018-02-21 Molecular prevalence and phylogenetic analysis of Theileria annulata and Trypanosoma evansi in cattle in Northern Tunisia Sallemi, Souhir Rjeibi, Mohamed R. Rouatbi, Mariem Amairia, Safa Ben Said, Mourad Khamassi Khbou, Madiha Gharbi, Mohamed Vet Med Sci Original Articles The present study aimed to estimate the molecular prevalence of Theileria annulata and Trypanosoma evansi infection in cattle in Northern Tunisia. A total number of 96 cattle from five farms were evaluated. T. annulata and T. evansi prevalences were 61% [56/66] and 10% [7/13], respectively, at a confidence interval (CI) of 95%, while co‐infection was present in 6% [4/8] of the tested animals at a CI of 95%. There was a significant correlation between age and the prevalence of T. annulata infection, whereas, there was no significant association shown with the age of cattle and T. evansi infection. Sequence and phylogenetic analyses showed that the T. annulata Tams1 gene and T. evansi ITS1 rDNA gene were highly conserved with 97.1–100% and 98.3–100% sequence identity, respectively. John Wiley and Sons Inc. 2017-11-29 /pmc/articles/PMC5813107/ /pubmed/29468077 http://dx.doi.org/10.1002/vms3.79 Text en © 2017 The Authors. Veterinary Medicine and Science Published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs (http://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
Sallemi, Souhir
Rjeibi, Mohamed R.
Rouatbi, Mariem
Amairia, Safa
Ben Said, Mourad
Khamassi Khbou, Madiha
Gharbi, Mohamed
Molecular prevalence and phylogenetic analysis of Theileria annulata and Trypanosoma evansi in cattle in Northern Tunisia
title Molecular prevalence and phylogenetic analysis of Theileria annulata and Trypanosoma evansi in cattle in Northern Tunisia
title_full Molecular prevalence and phylogenetic analysis of Theileria annulata and Trypanosoma evansi in cattle in Northern Tunisia
title_fullStr Molecular prevalence and phylogenetic analysis of Theileria annulata and Trypanosoma evansi in cattle in Northern Tunisia
title_full_unstemmed Molecular prevalence and phylogenetic analysis of Theileria annulata and Trypanosoma evansi in cattle in Northern Tunisia
title_short Molecular prevalence and phylogenetic analysis of Theileria annulata and Trypanosoma evansi in cattle in Northern Tunisia
title_sort molecular prevalence and phylogenetic analysis of theileria annulata and trypanosoma evansi in cattle in northern tunisia
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5813107/
https://www.ncbi.nlm.nih.gov/pubmed/29468077
http://dx.doi.org/10.1002/vms3.79
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