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Cinacalcet Rectifies Hypercalcemia in a Patient With Familial Hypocalciuric Hypercalcemia Type 2 (FHH2) Caused by a Germline Loss‐of‐Function Gα(11) Mutation

G‐protein subunit α‐11 (Gα(11)) couples the calcium‐sensing receptor (CaSR) to phospholipase C (PLC)‐mediated intracellular calcium (Ca(2+) (i)) and mitogen‐activated protein kinase (MAPK) signaling, which in the parathyroid glands and kidneys regulates parathyroid hormone release and urinary calciu...

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Autores principales: Gorvin, Caroline M, Hannan, Fadil M, Cranston, Treena, Valta, Helena, Makitie, Outi, Schalin‐Jantti, Camilla, Thakker, Rajesh V
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5813271/
https://www.ncbi.nlm.nih.gov/pubmed/28833550
http://dx.doi.org/10.1002/jbmr.3241
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author Gorvin, Caroline M
Hannan, Fadil M
Cranston, Treena
Valta, Helena
Makitie, Outi
Schalin‐Jantti, Camilla
Thakker, Rajesh V
author_facet Gorvin, Caroline M
Hannan, Fadil M
Cranston, Treena
Valta, Helena
Makitie, Outi
Schalin‐Jantti, Camilla
Thakker, Rajesh V
author_sort Gorvin, Caroline M
collection PubMed
description G‐protein subunit α‐11 (Gα(11)) couples the calcium‐sensing receptor (CaSR) to phospholipase C (PLC)‐mediated intracellular calcium (Ca(2+) (i)) and mitogen‐activated protein kinase (MAPK) signaling, which in the parathyroid glands and kidneys regulates parathyroid hormone release and urinary calcium excretion, respectively. Heterozygous germline loss‐of‐function Gα(11) mutations cause familial hypocalciuric hypercalcemia type 2 (FHH2), for which effective therapies are currently not available. Here, we report a novel heterozygous Gα(11) germline mutation, Phe220Ser, which was associated with hypercalcemia in a family with FHH2. Homology modeling showed the wild‐type (WT) Phe220 nonpolar residue to form part of a cluster of hydrophobic residues within a highly conserved cleft region of Gα(11), which binds to and activates PLC; and predicted that substitution of Phe220 with the mutant Ser220 polar hydrophilic residue would disrupt PLC‐mediated signaling. In vitro studies involving transient transfection of WT and mutant Gα(11) proteins into HEK293 cells, which express the CaSR, showed the mutant Ser220 Gα(11) protein to impair CaSR‐mediated Ca(2+) (i) and extracellular signal‐regulated kinase 1/2 (ERK) MAPK signaling, consistent with diminished activation of PLC. Furthermore, engineered mutagenesis studies demonstrated that loss of hydrophobicity within the Gα(11) cleft region also impaired signaling by PLC. The loss‐of‐function associated with the Ser220 Gα(11) mutant was rectified by treatment of cells with cinacalcet, which is a CaSR‐positive allosteric modulator. Furthermore, in vivo administration of cinacalcet to the proband harboring the Phe220Ser Gα(11) mutation, normalized serum ionized calcium concentrations. Thus, our studies, which report a novel Gα(11) germline mutation (Phe220Ser) in a family with FHH2, reveal the importance of the Gα(11) hydrophobic cleft region for CaSR‐mediated activation of PLC, and show that allosteric CaSR modulation can rectify the loss‐of‐function Phe220Ser mutation and ameliorate the hypercalcemia associated with FHH2. © 2017 The Authors. Journal of Bone and Mineral Research Published by Wiley Periodicals Inc.
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spelling pubmed-58132712018-02-21 Cinacalcet Rectifies Hypercalcemia in a Patient With Familial Hypocalciuric Hypercalcemia Type 2 (FHH2) Caused by a Germline Loss‐of‐Function Gα(11) Mutation Gorvin, Caroline M Hannan, Fadil M Cranston, Treena Valta, Helena Makitie, Outi Schalin‐Jantti, Camilla Thakker, Rajesh V J Bone Miner Res Original Article G‐protein subunit α‐11 (Gα(11)) couples the calcium‐sensing receptor (CaSR) to phospholipase C (PLC)‐mediated intracellular calcium (Ca(2+) (i)) and mitogen‐activated protein kinase (MAPK) signaling, which in the parathyroid glands and kidneys regulates parathyroid hormone release and urinary calcium excretion, respectively. Heterozygous germline loss‐of‐function Gα(11) mutations cause familial hypocalciuric hypercalcemia type 2 (FHH2), for which effective therapies are currently not available. Here, we report a novel heterozygous Gα(11) germline mutation, Phe220Ser, which was associated with hypercalcemia in a family with FHH2. Homology modeling showed the wild‐type (WT) Phe220 nonpolar residue to form part of a cluster of hydrophobic residues within a highly conserved cleft region of Gα(11), which binds to and activates PLC; and predicted that substitution of Phe220 with the mutant Ser220 polar hydrophilic residue would disrupt PLC‐mediated signaling. In vitro studies involving transient transfection of WT and mutant Gα(11) proteins into HEK293 cells, which express the CaSR, showed the mutant Ser220 Gα(11) protein to impair CaSR‐mediated Ca(2+) (i) and extracellular signal‐regulated kinase 1/2 (ERK) MAPK signaling, consistent with diminished activation of PLC. Furthermore, engineered mutagenesis studies demonstrated that loss of hydrophobicity within the Gα(11) cleft region also impaired signaling by PLC. The loss‐of‐function associated with the Ser220 Gα(11) mutant was rectified by treatment of cells with cinacalcet, which is a CaSR‐positive allosteric modulator. Furthermore, in vivo administration of cinacalcet to the proband harboring the Phe220Ser Gα(11) mutation, normalized serum ionized calcium concentrations. Thus, our studies, which report a novel Gα(11) germline mutation (Phe220Ser) in a family with FHH2, reveal the importance of the Gα(11) hydrophobic cleft region for CaSR‐mediated activation of PLC, and show that allosteric CaSR modulation can rectify the loss‐of‐function Phe220Ser mutation and ameliorate the hypercalcemia associated with FHH2. © 2017 The Authors. Journal of Bone and Mineral Research Published by Wiley Periodicals Inc. John Wiley and Sons Inc. 2017-09-22 2018-01 /pmc/articles/PMC5813271/ /pubmed/28833550 http://dx.doi.org/10.1002/jbmr.3241 Text en © 2017 The Authors. Journal of Bone and Mineral Research Published by Wiley Periodicals Inc. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Gorvin, Caroline M
Hannan, Fadil M
Cranston, Treena
Valta, Helena
Makitie, Outi
Schalin‐Jantti, Camilla
Thakker, Rajesh V
Cinacalcet Rectifies Hypercalcemia in a Patient With Familial Hypocalciuric Hypercalcemia Type 2 (FHH2) Caused by a Germline Loss‐of‐Function Gα(11) Mutation
title Cinacalcet Rectifies Hypercalcemia in a Patient With Familial Hypocalciuric Hypercalcemia Type 2 (FHH2) Caused by a Germline Loss‐of‐Function Gα(11) Mutation
title_full Cinacalcet Rectifies Hypercalcemia in a Patient With Familial Hypocalciuric Hypercalcemia Type 2 (FHH2) Caused by a Germline Loss‐of‐Function Gα(11) Mutation
title_fullStr Cinacalcet Rectifies Hypercalcemia in a Patient With Familial Hypocalciuric Hypercalcemia Type 2 (FHH2) Caused by a Germline Loss‐of‐Function Gα(11) Mutation
title_full_unstemmed Cinacalcet Rectifies Hypercalcemia in a Patient With Familial Hypocalciuric Hypercalcemia Type 2 (FHH2) Caused by a Germline Loss‐of‐Function Gα(11) Mutation
title_short Cinacalcet Rectifies Hypercalcemia in a Patient With Familial Hypocalciuric Hypercalcemia Type 2 (FHH2) Caused by a Germline Loss‐of‐Function Gα(11) Mutation
title_sort cinacalcet rectifies hypercalcemia in a patient with familial hypocalciuric hypercalcemia type 2 (fhh2) caused by a germline loss‐of‐function gα(11) mutation
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5813271/
https://www.ncbi.nlm.nih.gov/pubmed/28833550
http://dx.doi.org/10.1002/jbmr.3241
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