Letter to the editor: blood processing and sample storage have negligible effects on methylation

DNA methylation is a dynamic epigenetic mechanism. Researchers aiming to assess archived DNA samples are expressing concern about the effect of technical factors on methylation, as this may confound results. We reviewed recent reports examining this issue in blood samples and concluded that variatio...

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Autores principales: Groen, Kira, Lea, Rodney A., Maltby, Vicki E., Scott, Rodney J., Lechner-Scott, Jeannette
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Letter to the Editor
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5813389/
https://www.ncbi.nlm.nih.gov/pubmed/29456766
http://dx.doi.org/10.1186/s13148-018-0455-6
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author Groen, Kira
Lea, Rodney A.
Maltby, Vicki E.
Scott, Rodney J.
Lechner-Scott, Jeannette
author_facet Groen, Kira
Lea, Rodney A.
Maltby, Vicki E.
Scott, Rodney J.
Lechner-Scott, Jeannette
author_sort Groen, Kira
collection PubMed
description DNA methylation is a dynamic epigenetic mechanism. Researchers aiming to assess archived DNA samples are expressing concern about the effect of technical factors on methylation, as this may confound results. We reviewed recent reports examining this issue in blood samples and concluded that variation in collection, storage, and processing of blood DNA confers negligible effects on both global methylation and methylation status of specific genes. These results are concordant with studies that have investigated the effect of sample storage and processing on methylation in other tissues, such as tumour, sperm, and placenta samples.
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spelling pubmed-58133892018-02-16 Letter to the editor: blood processing and sample storage have negligible effects on methylation Groen, Kira Lea, Rodney A. Maltby, Vicki E. Scott, Rodney J. Lechner-Scott, Jeannette Clin Epigenetics Letter to the Editor DNA methylation is a dynamic epigenetic mechanism. Researchers aiming to assess archived DNA samples are expressing concern about the effect of technical factors on methylation, as this may confound results. We reviewed recent reports examining this issue in blood samples and concluded that variation in collection, storage, and processing of blood DNA confers negligible effects on both global methylation and methylation status of specific genes. These results are concordant with studies that have investigated the effect of sample storage and processing on methylation in other tissues, such as tumour, sperm, and placenta samples. BioMed Central 2018-02-14 /pmc/articles/PMC5813389/ /pubmed/29456766 http://dx.doi.org/10.1186/s13148-018-0455-6 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Letter to the Editor
Groen, Kira
Lea, Rodney A.
Maltby, Vicki E.
Scott, Rodney J.
Lechner-Scott, Jeannette
Letter to the editor: blood processing and sample storage have negligible effects on methylation
title Letter to the editor: blood processing and sample storage have negligible effects on methylation
title_full Letter to the editor: blood processing and sample storage have negligible effects on methylation
title_fullStr Letter to the editor: blood processing and sample storage have negligible effects on methylation
title_full_unstemmed Letter to the editor: blood processing and sample storage have negligible effects on methylation
title_short Letter to the editor: blood processing and sample storage have negligible effects on methylation
title_sort letter to the editor: blood processing and sample storage have negligible effects on methylation
topic Letter to the Editor
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5813389/
https://www.ncbi.nlm.nih.gov/pubmed/29456766
http://dx.doi.org/10.1186/s13148-018-0455-6
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