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Porcine Wharton’s jelly cells distribute throughout the body after intraperitoneal injection
BACKGROUND: Wharton's jelly cells (WJCs) have multiple differentiation potentials and are easily harvested in large numbers. WJCs are well tolerated in allogeneic environments and there is a growing list of their therapeutic effects. Most therapies require administering large numbers of cells a...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5813394/ https://www.ncbi.nlm.nih.gov/pubmed/29444715 http://dx.doi.org/10.1186/s13287-018-0775-7 |
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author | Packthongsuk, Kreeson Rathbun, Theresa Troyer, Deryl Davis, Duane L. |
author_facet | Packthongsuk, Kreeson Rathbun, Theresa Troyer, Deryl Davis, Duane L. |
author_sort | Packthongsuk, Kreeson |
collection | PubMed |
description | BACKGROUND: Wharton's jelly cells (WJCs) have multiple differentiation potentials and are easily harvested in large numbers. WJCs are well tolerated in allogeneic environments and there is a growing list of their therapeutic effects. Most therapies require administering large numbers of cells and this is generally accomplished by intravenous injection. Here, we studied the locations of porcine WJCs in immune-competent, allogeneic hosts after intraperitoneal (IP) injection. METHODS: Male porcine WJCs were administered to female neonatal piglets by IP injection. The location of transplanted cells was examined at 6 h, 24 h, and 7 days after administration using confocal microscopy and polymerase chain reaction (PCR). Transplanted cells were also retrieved from the intestines of recipients and were cultured. Previously transplanted cells were identified by fluorescence in-situ hybridization (FISH) using a Y-chromosome probe. RESULTS: Allogeneic cells were identified in the small and large intestine, stomach, liver, spleen, diaphragm, omentum, kidney, pancreas, mesenteric lymph nodes, heart, lungs, uterus, bladder, and skeletal muscle. Male cells (SRY positive) were found in cultures of cells harvested from the intestinal mucosa 1 week after administration of male porcine WJCs. CONCLUSIONS: Our results show that porcine WJCs distribute widely to the organs in immunocompetent allogeneic hosts after IP administration. They may distribute through the lymphatics initially, and a prominent site of incorporation is the mucosa of the gastrointestinal tract. In that location they could function in the niche of endogenous stem cells and provide secretory products to cells in the tissue damaged by intestinal disease. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13287-018-0775-7) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5813394 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-58133942018-02-16 Porcine Wharton’s jelly cells distribute throughout the body after intraperitoneal injection Packthongsuk, Kreeson Rathbun, Theresa Troyer, Deryl Davis, Duane L. Stem Cell Res Ther Research BACKGROUND: Wharton's jelly cells (WJCs) have multiple differentiation potentials and are easily harvested in large numbers. WJCs are well tolerated in allogeneic environments and there is a growing list of their therapeutic effects. Most therapies require administering large numbers of cells and this is generally accomplished by intravenous injection. Here, we studied the locations of porcine WJCs in immune-competent, allogeneic hosts after intraperitoneal (IP) injection. METHODS: Male porcine WJCs were administered to female neonatal piglets by IP injection. The location of transplanted cells was examined at 6 h, 24 h, and 7 days after administration using confocal microscopy and polymerase chain reaction (PCR). Transplanted cells were also retrieved from the intestines of recipients and were cultured. Previously transplanted cells were identified by fluorescence in-situ hybridization (FISH) using a Y-chromosome probe. RESULTS: Allogeneic cells were identified in the small and large intestine, stomach, liver, spleen, diaphragm, omentum, kidney, pancreas, mesenteric lymph nodes, heart, lungs, uterus, bladder, and skeletal muscle. Male cells (SRY positive) were found in cultures of cells harvested from the intestinal mucosa 1 week after administration of male porcine WJCs. CONCLUSIONS: Our results show that porcine WJCs distribute widely to the organs in immunocompetent allogeneic hosts after IP administration. They may distribute through the lymphatics initially, and a prominent site of incorporation is the mucosa of the gastrointestinal tract. In that location they could function in the niche of endogenous stem cells and provide secretory products to cells in the tissue damaged by intestinal disease. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13287-018-0775-7) contains supplementary material, which is available to authorized users. BioMed Central 2018-02-14 /pmc/articles/PMC5813394/ /pubmed/29444715 http://dx.doi.org/10.1186/s13287-018-0775-7 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Packthongsuk, Kreeson Rathbun, Theresa Troyer, Deryl Davis, Duane L. Porcine Wharton’s jelly cells distribute throughout the body after intraperitoneal injection |
title | Porcine Wharton’s jelly cells distribute throughout the body after intraperitoneal injection |
title_full | Porcine Wharton’s jelly cells distribute throughout the body after intraperitoneal injection |
title_fullStr | Porcine Wharton’s jelly cells distribute throughout the body after intraperitoneal injection |
title_full_unstemmed | Porcine Wharton’s jelly cells distribute throughout the body after intraperitoneal injection |
title_short | Porcine Wharton’s jelly cells distribute throughout the body after intraperitoneal injection |
title_sort | porcine wharton’s jelly cells distribute throughout the body after intraperitoneal injection |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5813394/ https://www.ncbi.nlm.nih.gov/pubmed/29444715 http://dx.doi.org/10.1186/s13287-018-0775-7 |
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