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Effect of tt-farnesol and myricetin on in vitro biofilm formed by Streptococcus mutans and Candida albicans

BACKGROUND: Dental caries is considered a multifactorial disease, in which microorganisms play an important role. The diet is decisive in the biofilm formation because it provides the necessary resources for cellular growth and exopolysaccharides synthesis. Exopolysaccharides are the main components...

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Autores principales: Rocha, Guilherme Roncari, Florez Salamanca, Elkin Jahir, de Barros, Ana Letícia, Lobo, Carmélia Isabel Vitorino, Klein, Marlise Inêz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5813409/
https://www.ncbi.nlm.nih.gov/pubmed/29444673
http://dx.doi.org/10.1186/s12906-018-2132-x
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author Rocha, Guilherme Roncari
Florez Salamanca, Elkin Jahir
de Barros, Ana Letícia
Lobo, Carmélia Isabel Vitorino
Klein, Marlise Inêz
author_facet Rocha, Guilherme Roncari
Florez Salamanca, Elkin Jahir
de Barros, Ana Letícia
Lobo, Carmélia Isabel Vitorino
Klein, Marlise Inêz
author_sort Rocha, Guilherme Roncari
collection PubMed
description BACKGROUND: Dental caries is considered a multifactorial disease, in which microorganisms play an important role. The diet is decisive in the biofilm formation because it provides the necessary resources for cellular growth and exopolysaccharides synthesis. Exopolysaccharides are the main components of the extracellular matrix (ECM). The ECM provides a 3D structure, support for the microorganisms and form diffusion-limited environments (acidic niches) that cause demineralization of the dental enamel. Streptococcus mutans is the main producer of exopolysaccharides. Candida albicans is detected together with S. mutans in biofilms associated with severe caries lesions. Thus, this study aimed to determine the effect of tt-farnesol and myricetin topical treatments on cariogenic biofilms formed by Streptococcus mutans and Candida albicans. METHODS: In vitro dual-species biofilms were grown on saliva-coated hydroxyapatite discs, using tryptone-yeast extract broth with 1% sucrose (37 °C, 5% CO(2)). Twice-daily topical treatments were performed with: vehicle (ethanol 15%, negative control), 2 mM myricetin, 4 mM tt-farnesol, myricetin + tt-farnesol, myricetin + tt-farnesol + fluoride (250 ppm), fluoride, and chlorhexidine digluconate (0.12%; positive control). After 67 h, biofilms were evaluated to determine biofilm biomass, microbial population, and water-soluble and -insoluble exopolysaccharides in the ECM. RESULTS: Only the positive control yielded a reduced quantity of biomass and microbial population, while tt-farnesol treatment was the least efficient in reducing C. albicans population. The combination therapy myricetin + farnesol + fluoride significantly reduced water-soluble exopolysaccharides in the ECM (vs. negative control; p < 0.05; ANOVA one-way, followed by Tukey’s test), similarly to the positive control. CONCLUSIONS: Therefore, the combination therapy negatively influenced an important virulence trait of cariogenic biofilms. However, the concentrations of both myricetin and tt-farnesol should be increased to produce a more pronounced effect to control these biofilms.
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spelling pubmed-58134092018-02-16 Effect of tt-farnesol and myricetin on in vitro biofilm formed by Streptococcus mutans and Candida albicans Rocha, Guilherme Roncari Florez Salamanca, Elkin Jahir de Barros, Ana Letícia Lobo, Carmélia Isabel Vitorino Klein, Marlise Inêz BMC Complement Altern Med Research Article BACKGROUND: Dental caries is considered a multifactorial disease, in which microorganisms play an important role. The diet is decisive in the biofilm formation because it provides the necessary resources for cellular growth and exopolysaccharides synthesis. Exopolysaccharides are the main components of the extracellular matrix (ECM). The ECM provides a 3D structure, support for the microorganisms and form diffusion-limited environments (acidic niches) that cause demineralization of the dental enamel. Streptococcus mutans is the main producer of exopolysaccharides. Candida albicans is detected together with S. mutans in biofilms associated with severe caries lesions. Thus, this study aimed to determine the effect of tt-farnesol and myricetin topical treatments on cariogenic biofilms formed by Streptococcus mutans and Candida albicans. METHODS: In vitro dual-species biofilms were grown on saliva-coated hydroxyapatite discs, using tryptone-yeast extract broth with 1% sucrose (37 °C, 5% CO(2)). Twice-daily topical treatments were performed with: vehicle (ethanol 15%, negative control), 2 mM myricetin, 4 mM tt-farnesol, myricetin + tt-farnesol, myricetin + tt-farnesol + fluoride (250 ppm), fluoride, and chlorhexidine digluconate (0.12%; positive control). After 67 h, biofilms were evaluated to determine biofilm biomass, microbial population, and water-soluble and -insoluble exopolysaccharides in the ECM. RESULTS: Only the positive control yielded a reduced quantity of biomass and microbial population, while tt-farnesol treatment was the least efficient in reducing C. albicans population. The combination therapy myricetin + farnesol + fluoride significantly reduced water-soluble exopolysaccharides in the ECM (vs. negative control; p < 0.05; ANOVA one-way, followed by Tukey’s test), similarly to the positive control. CONCLUSIONS: Therefore, the combination therapy negatively influenced an important virulence trait of cariogenic biofilms. However, the concentrations of both myricetin and tt-farnesol should be increased to produce a more pronounced effect to control these biofilms. BioMed Central 2018-02-14 /pmc/articles/PMC5813409/ /pubmed/29444673 http://dx.doi.org/10.1186/s12906-018-2132-x Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Rocha, Guilherme Roncari
Florez Salamanca, Elkin Jahir
de Barros, Ana Letícia
Lobo, Carmélia Isabel Vitorino
Klein, Marlise Inêz
Effect of tt-farnesol and myricetin on in vitro biofilm formed by Streptococcus mutans and Candida albicans
title Effect of tt-farnesol and myricetin on in vitro biofilm formed by Streptococcus mutans and Candida albicans
title_full Effect of tt-farnesol and myricetin on in vitro biofilm formed by Streptococcus mutans and Candida albicans
title_fullStr Effect of tt-farnesol and myricetin on in vitro biofilm formed by Streptococcus mutans and Candida albicans
title_full_unstemmed Effect of tt-farnesol and myricetin on in vitro biofilm formed by Streptococcus mutans and Candida albicans
title_short Effect of tt-farnesol and myricetin on in vitro biofilm formed by Streptococcus mutans and Candida albicans
title_sort effect of tt-farnesol and myricetin on in vitro biofilm formed by streptococcus mutans and candida albicans
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5813409/
https://www.ncbi.nlm.nih.gov/pubmed/29444673
http://dx.doi.org/10.1186/s12906-018-2132-x
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