A Novel Approach to Data Collection for Difficult Structures: Data Management for Large Numbers of Crystals with the BLEND Software

The present article describes how to use the computer program BLEND to help assemble complete datasets for the solution of macromolecular structures, starting from partial or complete datasets, derived from data collection from multiple crystals. The program is demonstrated on more than two hundred...

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Autores principales: Mylona, Anastasia, Carr, Stephen, Aller, Pierre, Moraes, Isabel, Treisman, Richard, Evans, Gwyndaf, Foadi, James
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5813789/
https://www.ncbi.nlm.nih.gov/pubmed/29456874
http://dx.doi.org/10.3390/cryst7080242
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author Mylona, Anastasia
Carr, Stephen
Aller, Pierre
Moraes, Isabel
Treisman, Richard
Evans, Gwyndaf
Foadi, James
author_facet Mylona, Anastasia
Carr, Stephen
Aller, Pierre
Moraes, Isabel
Treisman, Richard
Evans, Gwyndaf
Foadi, James
author_sort Mylona, Anastasia
collection PubMed
description The present article describes how to use the computer program BLEND to help assemble complete datasets for the solution of macromolecular structures, starting from partial or complete datasets, derived from data collection from multiple crystals. The program is demonstrated on more than two hundred X-ray diffraction datasets obtained from 50 crystals of a complex formed between the SRF transcription factor, its cognate DNA, and a peptide from the SRF cofactor MRTF-A. This structure is currently in the process of being fully solved. While full details of the structure are not yet available, the repeated application of BLEND on data from this structure, as they have become available, has made it possible to produce electron density maps clear enough to visualise the potential location of MRTF sequences.
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spelling pubmed-58137892018-02-15 A Novel Approach to Data Collection for Difficult Structures: Data Management for Large Numbers of Crystals with the BLEND Software Mylona, Anastasia Carr, Stephen Aller, Pierre Moraes, Isabel Treisman, Richard Evans, Gwyndaf Foadi, James Crystals (Basel) Article The present article describes how to use the computer program BLEND to help assemble complete datasets for the solution of macromolecular structures, starting from partial or complete datasets, derived from data collection from multiple crystals. The program is demonstrated on more than two hundred X-ray diffraction datasets obtained from 50 crystals of a complex formed between the SRF transcription factor, its cognate DNA, and a peptide from the SRF cofactor MRTF-A. This structure is currently in the process of being fully solved. While full details of the structure are not yet available, the repeated application of BLEND on data from this structure, as they have become available, has made it possible to produce electron density maps clear enough to visualise the potential location of MRTF sequences. 2017-08-04 /pmc/articles/PMC5813789/ /pubmed/29456874 http://dx.doi.org/10.3390/cryst7080242 Text en http://creativecommons.org/licenses/by/4.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mylona, Anastasia
Carr, Stephen
Aller, Pierre
Moraes, Isabel
Treisman, Richard
Evans, Gwyndaf
Foadi, James
A Novel Approach to Data Collection for Difficult Structures: Data Management for Large Numbers of Crystals with the BLEND Software
title A Novel Approach to Data Collection for Difficult Structures: Data Management for Large Numbers of Crystals with the BLEND Software
title_full A Novel Approach to Data Collection for Difficult Structures: Data Management for Large Numbers of Crystals with the BLEND Software
title_fullStr A Novel Approach to Data Collection for Difficult Structures: Data Management for Large Numbers of Crystals with the BLEND Software
title_full_unstemmed A Novel Approach to Data Collection for Difficult Structures: Data Management for Large Numbers of Crystals with the BLEND Software
title_short A Novel Approach to Data Collection for Difficult Structures: Data Management for Large Numbers of Crystals with the BLEND Software
title_sort novel approach to data collection for difficult structures: data management for large numbers of crystals with the blend software
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5813789/
https://www.ncbi.nlm.nih.gov/pubmed/29456874
http://dx.doi.org/10.3390/cryst7080242
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