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Ten Basic Rules of Antibody Validation
The quality of research antibodies is an issue for decades. Although several papers have been published to improve the situation, their impact seems to be limited. This publication makes the effort to simplify the description of validation criteria in a way that the occasional antibody user is able...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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SAGE Publications
2018
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5813849/ https://www.ncbi.nlm.nih.gov/pubmed/29467569 http://dx.doi.org/10.1177/1177390118757462 |
| _version_ | 1783300237621723136 |
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| author | Weller, Michael G |
| author_facet | Weller, Michael G |
| author_sort | Weller, Michael G |
| collection | PubMed |
| description | The quality of research antibodies is an issue for decades. Although several papers have been published to improve the situation, their impact seems to be limited. This publication makes the effort to simplify the description of validation criteria in a way that the occasional antibody user is able to assess the validation level of an immunochemical reagent. A simple, 1-page checklist is supplied for the practical application of these criteria. |
| format | Online Article Text |
| id | pubmed-5813849 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2018 |
| publisher | SAGE Publications |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-58138492018-02-21 Ten Basic Rules of Antibody Validation Weller, Michael G Anal Chem Insights Review The quality of research antibodies is an issue for decades. Although several papers have been published to improve the situation, their impact seems to be limited. This publication makes the effort to simplify the description of validation criteria in a way that the occasional antibody user is able to assess the validation level of an immunochemical reagent. A simple, 1-page checklist is supplied for the practical application of these criteria. SAGE Publications 2018-02-08 /pmc/articles/PMC5813849/ /pubmed/29467569 http://dx.doi.org/10.1177/1177390118757462 Text en © The Author(s) 2018 http://www.creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
| spellingShingle | Review Weller, Michael G Ten Basic Rules of Antibody Validation |
| title | Ten Basic Rules of Antibody Validation |
| title_full | Ten Basic Rules of Antibody Validation |
| title_fullStr | Ten Basic Rules of Antibody Validation |
| title_full_unstemmed | Ten Basic Rules of Antibody Validation |
| title_short | Ten Basic Rules of Antibody Validation |
| title_sort | ten basic rules of antibody validation |
| topic | Review |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5813849/ https://www.ncbi.nlm.nih.gov/pubmed/29467569 http://dx.doi.org/10.1177/1177390118757462 |
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