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Molecular analyses of two bacterial sampling methods in ligature‐induced periodontitis in rats

The prevalence profile of periodontal pathogens in dental plaque can vary as a function of the detection method; however, the sampling technique may also play a role in determining dental plaque microbial profiles. We sought to determine the bacterial composition comparing two sampling methods, one...

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Autores principales: Fontana, Carla Raquel, Grecco, Clovis, Bagnato, Vanderlei Salvador, de Freitas, Laura Marise, Boussios, Constantinos I., Soukos, Nikolaos S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5813890/
https://www.ncbi.nlm.nih.gov/pubmed/29744211
http://dx.doi.org/10.1002/cre2.98
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author Fontana, Carla Raquel
Grecco, Clovis
Bagnato, Vanderlei Salvador
de Freitas, Laura Marise
Boussios, Constantinos I.
Soukos, Nikolaos S.
author_facet Fontana, Carla Raquel
Grecco, Clovis
Bagnato, Vanderlei Salvador
de Freitas, Laura Marise
Boussios, Constantinos I.
Soukos, Nikolaos S.
author_sort Fontana, Carla Raquel
collection PubMed
description The prevalence profile of periodontal pathogens in dental plaque can vary as a function of the detection method; however, the sampling technique may also play a role in determining dental plaque microbial profiles. We sought to determine the bacterial composition comparing two sampling methods, one well stablished and a new one proposed here. In this study, a ligature‐induced periodontitis model was used in 30 rats. Twenty‐seven days later, ligatures were removed and microbiological samples were obtained directly from the ligatures as well as from the periodontal pockets using absorbent paper points. Microbial analysis was performed using DNA probes to a panel of 40 periodontal species in the checkerboard assay. The bacterial composition patterns were similar for both sampling methods. However, detection levels for all species were markedly higher for ligatures compared with paper points. Ligature samples provided more bacterial counts than paper points, suggesting that the technique for induction of periodontitis could also be applied for sampling in rats. Our findings may be helpful in designing studies of induced periodontal disease‐associated microbiota.
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spelling pubmed-58138902018-05-09 Molecular analyses of two bacterial sampling methods in ligature‐induced periodontitis in rats Fontana, Carla Raquel Grecco, Clovis Bagnato, Vanderlei Salvador de Freitas, Laura Marise Boussios, Constantinos I. Soukos, Nikolaos S. Clin Exp Dent Res Original Articles The prevalence profile of periodontal pathogens in dental plaque can vary as a function of the detection method; however, the sampling technique may also play a role in determining dental plaque microbial profiles. We sought to determine the bacterial composition comparing two sampling methods, one well stablished and a new one proposed here. In this study, a ligature‐induced periodontitis model was used in 30 rats. Twenty‐seven days later, ligatures were removed and microbiological samples were obtained directly from the ligatures as well as from the periodontal pockets using absorbent paper points. Microbial analysis was performed using DNA probes to a panel of 40 periodontal species in the checkerboard assay. The bacterial composition patterns were similar for both sampling methods. However, detection levels for all species were markedly higher for ligatures compared with paper points. Ligature samples provided more bacterial counts than paper points, suggesting that the technique for induction of periodontitis could also be applied for sampling in rats. Our findings may be helpful in designing studies of induced periodontal disease‐associated microbiota. John Wiley and Sons Inc. 2018-02-15 /pmc/articles/PMC5813890/ /pubmed/29744211 http://dx.doi.org/10.1002/cre2.98 Text en ©2018 The Authors. Clinical and Experimental Dental Research published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Fontana, Carla Raquel
Grecco, Clovis
Bagnato, Vanderlei Salvador
de Freitas, Laura Marise
Boussios, Constantinos I.
Soukos, Nikolaos S.
Molecular analyses of two bacterial sampling methods in ligature‐induced periodontitis in rats
title Molecular analyses of two bacterial sampling methods in ligature‐induced periodontitis in rats
title_full Molecular analyses of two bacterial sampling methods in ligature‐induced periodontitis in rats
title_fullStr Molecular analyses of two bacterial sampling methods in ligature‐induced periodontitis in rats
title_full_unstemmed Molecular analyses of two bacterial sampling methods in ligature‐induced periodontitis in rats
title_short Molecular analyses of two bacterial sampling methods in ligature‐induced periodontitis in rats
title_sort molecular analyses of two bacterial sampling methods in ligature‐induced periodontitis in rats
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5813890/
https://www.ncbi.nlm.nih.gov/pubmed/29744211
http://dx.doi.org/10.1002/cre2.98
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