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Functional imaging of the interaction between gut microbiota and the human host: A proof-of-concept clinical study evaluating novel use for 18F-FDG PET-CT

Recent data comparing germ-free to conventionally-raised mice demonstrated that energy homeostasis of colonocytes is dependent on gut microbiota through regulation of short chain fatty acids (SCFA) production and glucose utilization. We sought to evaluate 18F-FDG PET-CT as a novel technique for func...

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Autores principales: Boursi, Ben, Werner, Thomas J., Gholami, Saeid, Houshmand, Sina, Mamtani, Ronac, Lewis, James D., Wu, Gary D., Alavi, Abass, Yang, Yu-Xiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5813966/
https://www.ncbi.nlm.nih.gov/pubmed/29447210
http://dx.doi.org/10.1371/journal.pone.0192747
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author Boursi, Ben
Werner, Thomas J.
Gholami, Saeid
Houshmand, Sina
Mamtani, Ronac
Lewis, James D.
Wu, Gary D.
Alavi, Abass
Yang, Yu-Xiao
author_facet Boursi, Ben
Werner, Thomas J.
Gholami, Saeid
Houshmand, Sina
Mamtani, Ronac
Lewis, James D.
Wu, Gary D.
Alavi, Abass
Yang, Yu-Xiao
author_sort Boursi, Ben
collection PubMed
description Recent data comparing germ-free to conventionally-raised mice demonstrated that energy homeostasis of colonocytes is dependent on gut microbiota through regulation of short chain fatty acids (SCFA) production and glucose utilization. We sought to evaluate 18F-FDG PET-CT as a novel technique for functional imaging of alterations in glucose metabolism as a result of the interaction between the gut microbiota and the human host. We conducted a prospective study in healthy humans that underwent 18F-FDG PET-CT and sampling of the gut microbiota before and after orally administered broad-spectrum antibiotics. The primary outcomes were total and regional physiologic colonic 18F-FDG uptake (measured as the mean and max standardized uptake values [SUVmean and SUVmax]). The study demonstrated significant increases in physiologic colonic 18F-FDG uptake in all study participants following antibiotic treatment and a 4-5log reduction of gut bacterial load. The mean increase in SUVmax was 0.63±0.37 SD (p = 0.004) and the median increase was 0.42 with an IQR of 0.40–0.81. The mean increase in SUVmean was 0.31±0.24 SD (p = 0.01) and the median increase was 0.41 with an IQR of 0.06–0.55. A likely explanation for this phenomenon is a shift in colonocyte metabolism to glycolysis due to a shortage of SCFA.
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spelling pubmed-58139662018-03-02 Functional imaging of the interaction between gut microbiota and the human host: A proof-of-concept clinical study evaluating novel use for 18F-FDG PET-CT Boursi, Ben Werner, Thomas J. Gholami, Saeid Houshmand, Sina Mamtani, Ronac Lewis, James D. Wu, Gary D. Alavi, Abass Yang, Yu-Xiao PLoS One Research Article Recent data comparing germ-free to conventionally-raised mice demonstrated that energy homeostasis of colonocytes is dependent on gut microbiota through regulation of short chain fatty acids (SCFA) production and glucose utilization. We sought to evaluate 18F-FDG PET-CT as a novel technique for functional imaging of alterations in glucose metabolism as a result of the interaction between the gut microbiota and the human host. We conducted a prospective study in healthy humans that underwent 18F-FDG PET-CT and sampling of the gut microbiota before and after orally administered broad-spectrum antibiotics. The primary outcomes were total and regional physiologic colonic 18F-FDG uptake (measured as the mean and max standardized uptake values [SUVmean and SUVmax]). The study demonstrated significant increases in physiologic colonic 18F-FDG uptake in all study participants following antibiotic treatment and a 4-5log reduction of gut bacterial load. The mean increase in SUVmax was 0.63±0.37 SD (p = 0.004) and the median increase was 0.42 with an IQR of 0.40–0.81. The mean increase in SUVmean was 0.31±0.24 SD (p = 0.01) and the median increase was 0.41 with an IQR of 0.06–0.55. A likely explanation for this phenomenon is a shift in colonocyte metabolism to glycolysis due to a shortage of SCFA. Public Library of Science 2018-02-15 /pmc/articles/PMC5813966/ /pubmed/29447210 http://dx.doi.org/10.1371/journal.pone.0192747 Text en © 2018 Boursi et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Boursi, Ben
Werner, Thomas J.
Gholami, Saeid
Houshmand, Sina
Mamtani, Ronac
Lewis, James D.
Wu, Gary D.
Alavi, Abass
Yang, Yu-Xiao
Functional imaging of the interaction between gut microbiota and the human host: A proof-of-concept clinical study evaluating novel use for 18F-FDG PET-CT
title Functional imaging of the interaction between gut microbiota and the human host: A proof-of-concept clinical study evaluating novel use for 18F-FDG PET-CT
title_full Functional imaging of the interaction between gut microbiota and the human host: A proof-of-concept clinical study evaluating novel use for 18F-FDG PET-CT
title_fullStr Functional imaging of the interaction between gut microbiota and the human host: A proof-of-concept clinical study evaluating novel use for 18F-FDG PET-CT
title_full_unstemmed Functional imaging of the interaction between gut microbiota and the human host: A proof-of-concept clinical study evaluating novel use for 18F-FDG PET-CT
title_short Functional imaging of the interaction between gut microbiota and the human host: A proof-of-concept clinical study evaluating novel use for 18F-FDG PET-CT
title_sort functional imaging of the interaction between gut microbiota and the human host: a proof-of-concept clinical study evaluating novel use for 18f-fdg pet-ct
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5813966/
https://www.ncbi.nlm.nih.gov/pubmed/29447210
http://dx.doi.org/10.1371/journal.pone.0192747
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