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Zika virus like particles elicit protective antibodies in mice
Mosquito-borne Zika virus (ZIKV) typically causes a mild and self-limiting illness known as Zika fever. Since its recent emergence in 2014 in the American continent, ZIKV infection during pregnancy has been closely associated with a wide range of congenital abnormalities. To date, no vaccines or ant...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5814096/ https://www.ncbi.nlm.nih.gov/pubmed/29401460 http://dx.doi.org/10.1371/journal.pntd.0006210 |
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author | Salvo, Mauricio A. Kingstad-Bakke, Brock Salas-Quinchucua, Cristhian Camacho, Erwin Osorio, Jorge E. |
author_facet | Salvo, Mauricio A. Kingstad-Bakke, Brock Salas-Quinchucua, Cristhian Camacho, Erwin Osorio, Jorge E. |
author_sort | Salvo, Mauricio A. |
collection | PubMed |
description | Mosquito-borne Zika virus (ZIKV) typically causes a mild and self-limiting illness known as Zika fever. Since its recent emergence in 2014 in the American continent, ZIKV infection during pregnancy has been closely associated with a wide range of congenital abnormalities. To date, no vaccines or antivirals are publicly available. We developed Zika virus-like particles (VLPs) and evaluated their immunogenicity and protective efficacy in mouse models. ZIKV VLPs (ZIKVLPs) formulated with alum were injected into 6-8-week-old interferon deficient AG129 mice as well as wild type BALB/c mice. Control mice received PBS/alum. Animals were challenged with 200 PFU (>1000 AG129 LD50s) of ZIKV strain H/PF/2013. All vaccinated mice survived with no morbidity or weight loss while control animals either died at 9 days post challenge (AG129) or had increased viremia (BALB/c). Neutralizing antibodies were observed in all ZIKVLP vaccinated mice. The role of neutralizing antibodies in protecting mice was demonstrated by passive transfer. Our findings demonstrate the protective efficacy of the ZIKVLP vaccine and highlight the important role that neutralizing antibodies play in protection against ZIKV infection. |
format | Online Article Text |
id | pubmed-5814096 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-58140962018-03-15 Zika virus like particles elicit protective antibodies in mice Salvo, Mauricio A. Kingstad-Bakke, Brock Salas-Quinchucua, Cristhian Camacho, Erwin Osorio, Jorge E. PLoS Negl Trop Dis Research Article Mosquito-borne Zika virus (ZIKV) typically causes a mild and self-limiting illness known as Zika fever. Since its recent emergence in 2014 in the American continent, ZIKV infection during pregnancy has been closely associated with a wide range of congenital abnormalities. To date, no vaccines or antivirals are publicly available. We developed Zika virus-like particles (VLPs) and evaluated their immunogenicity and protective efficacy in mouse models. ZIKV VLPs (ZIKVLPs) formulated with alum were injected into 6-8-week-old interferon deficient AG129 mice as well as wild type BALB/c mice. Control mice received PBS/alum. Animals were challenged with 200 PFU (>1000 AG129 LD50s) of ZIKV strain H/PF/2013. All vaccinated mice survived with no morbidity or weight loss while control animals either died at 9 days post challenge (AG129) or had increased viremia (BALB/c). Neutralizing antibodies were observed in all ZIKVLP vaccinated mice. The role of neutralizing antibodies in protecting mice was demonstrated by passive transfer. Our findings demonstrate the protective efficacy of the ZIKVLP vaccine and highlight the important role that neutralizing antibodies play in protection against ZIKV infection. Public Library of Science 2018-02-05 /pmc/articles/PMC5814096/ /pubmed/29401460 http://dx.doi.org/10.1371/journal.pntd.0006210 Text en © 2018 Salvo et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Salvo, Mauricio A. Kingstad-Bakke, Brock Salas-Quinchucua, Cristhian Camacho, Erwin Osorio, Jorge E. Zika virus like particles elicit protective antibodies in mice |
title | Zika virus like particles elicit protective antibodies in mice |
title_full | Zika virus like particles elicit protective antibodies in mice |
title_fullStr | Zika virus like particles elicit protective antibodies in mice |
title_full_unstemmed | Zika virus like particles elicit protective antibodies in mice |
title_short | Zika virus like particles elicit protective antibodies in mice |
title_sort | zika virus like particles elicit protective antibodies in mice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5814096/ https://www.ncbi.nlm.nih.gov/pubmed/29401460 http://dx.doi.org/10.1371/journal.pntd.0006210 |
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