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Biocatalytic production of adipic acid from glucose using engineered Saccharomyces cerevisiae

Adipic acid is an important industrial chemical used in the synthesis of nylon-6,6. The commercial synthesis of adipic acid uses petroleum-derived benzene and releases significant quantities of greenhouse gases. Biocatalytic production of adipic acid from renewable feedstocks could potentially reduc...

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Autores principales: Raj, Kaushik, Partow, Siavash, Correia, Kevin, Khusnutdinova, Anna N., Yakunin, Alexander F., Mahadevan, Radhakrishnan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5814376/
https://www.ncbi.nlm.nih.gov/pubmed/29487800
http://dx.doi.org/10.1016/j.meteno.2018.02.001
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author Raj, Kaushik
Partow, Siavash
Correia, Kevin
Khusnutdinova, Anna N.
Yakunin, Alexander F.
Mahadevan, Radhakrishnan
author_facet Raj, Kaushik
Partow, Siavash
Correia, Kevin
Khusnutdinova, Anna N.
Yakunin, Alexander F.
Mahadevan, Radhakrishnan
author_sort Raj, Kaushik
collection PubMed
description Adipic acid is an important industrial chemical used in the synthesis of nylon-6,6. The commercial synthesis of adipic acid uses petroleum-derived benzene and releases significant quantities of greenhouse gases. Biocatalytic production of adipic acid from renewable feedstocks could potentially reduce the environmental damage and eliminate the need for fossil fuel precursors. Recently, we have demonstrated the first enzymatic hydrogenation of muconic acid to adipic acid using microbial enoate reductases (ERs) - complex iron-sulfur and flavin containing enzymes. In this work, we successfully expressed the Bacillus coagulans ER in a Saccharomyces cerevisiae strain producing muconic acid and developed a three-stage fermentation process enabling the synthesis of adipic acid from glucose. The ability to express active ERs and significant acid tolerance of S. cerevisiae highlight the applicability of the developed yeast strain for the biocatalytic production of adipic acid from renewable feedstocks.
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spelling pubmed-58143762018-02-27 Biocatalytic production of adipic acid from glucose using engineered Saccharomyces cerevisiae Raj, Kaushik Partow, Siavash Correia, Kevin Khusnutdinova, Anna N. Yakunin, Alexander F. Mahadevan, Radhakrishnan Metab Eng Commun Article Adipic acid is an important industrial chemical used in the synthesis of nylon-6,6. The commercial synthesis of adipic acid uses petroleum-derived benzene and releases significant quantities of greenhouse gases. Biocatalytic production of adipic acid from renewable feedstocks could potentially reduce the environmental damage and eliminate the need for fossil fuel precursors. Recently, we have demonstrated the first enzymatic hydrogenation of muconic acid to adipic acid using microbial enoate reductases (ERs) - complex iron-sulfur and flavin containing enzymes. In this work, we successfully expressed the Bacillus coagulans ER in a Saccharomyces cerevisiae strain producing muconic acid and developed a three-stage fermentation process enabling the synthesis of adipic acid from glucose. The ability to express active ERs and significant acid tolerance of S. cerevisiae highlight the applicability of the developed yeast strain for the biocatalytic production of adipic acid from renewable feedstocks. Elsevier 2018-02-03 /pmc/articles/PMC5814376/ /pubmed/29487800 http://dx.doi.org/10.1016/j.meteno.2018.02.001 Text en © 2018 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Raj, Kaushik
Partow, Siavash
Correia, Kevin
Khusnutdinova, Anna N.
Yakunin, Alexander F.
Mahadevan, Radhakrishnan
Biocatalytic production of adipic acid from glucose using engineered Saccharomyces cerevisiae
title Biocatalytic production of adipic acid from glucose using engineered Saccharomyces cerevisiae
title_full Biocatalytic production of adipic acid from glucose using engineered Saccharomyces cerevisiae
title_fullStr Biocatalytic production of adipic acid from glucose using engineered Saccharomyces cerevisiae
title_full_unstemmed Biocatalytic production of adipic acid from glucose using engineered Saccharomyces cerevisiae
title_short Biocatalytic production of adipic acid from glucose using engineered Saccharomyces cerevisiae
title_sort biocatalytic production of adipic acid from glucose using engineered saccharomyces cerevisiae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5814376/
https://www.ncbi.nlm.nih.gov/pubmed/29487800
http://dx.doi.org/10.1016/j.meteno.2018.02.001
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