Structural elucidation of a polysaccharide from Flammulina velutipes and its immunomodulation activities on mouse B lymphocytes

A novel polysaccharide FVPB2 was purified from fruiting bodies of Flammulina velutipes. Its structure was elucidated by monosaccharide composition and methylation analyses, UV-Visible and FTIR spectroscopy as well as NMR. FVPB2 was a homogeneous heteropolysaccharide (molecular weight ~ 1.50 × 10(4) ...

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Autores principales: Wang, Wen-Han, Zhang, Jing-Song, Feng, Ting, Deng, Jing, Lin, Chi-Chung, Fan, Hua, Yu, Wen-Juan, Bao, Hai-Ying, Jia, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5814432/
https://www.ncbi.nlm.nih.gov/pubmed/29449636
http://dx.doi.org/10.1038/s41598-018-21375-0
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author Wang, Wen-Han
Zhang, Jing-Song
Feng, Ting
Deng, Jing
Lin, Chi-Chung
Fan, Hua
Yu, Wen-Juan
Bao, Hai-Ying
Jia, Wei
author_facet Wang, Wen-Han
Zhang, Jing-Song
Feng, Ting
Deng, Jing
Lin, Chi-Chung
Fan, Hua
Yu, Wen-Juan
Bao, Hai-Ying
Jia, Wei
author_sort Wang, Wen-Han
collection PubMed
description A novel polysaccharide FVPB2 was purified from fruiting bodies of Flammulina velutipes. Its structure was elucidated by monosaccharide composition and methylation analyses, UV-Visible and FTIR spectroscopy as well as NMR. FVPB2 was a homogeneous heteropolysaccharide (molecular weight ~ 1.50 × 10(4) Da) containing D-galactose, D-mannose, L-fucose, and D-glucose at molar ratio of 1.9:1.2:1:2.5. In vitro immunomodulatory studies showed FVPB2 induced proliferation of mouse spleen lymphocytes in a dose-dependent manner. The levels of IgM and IgG, secreted by B cells, increased after FVPB2 treatment. So FVPB2 has potential to be a new important immunomodulatory nutraceutical.
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spelling pubmed-58144322018-02-21 Structural elucidation of a polysaccharide from Flammulina velutipes and its immunomodulation activities on mouse B lymphocytes Wang, Wen-Han Zhang, Jing-Song Feng, Ting Deng, Jing Lin, Chi-Chung Fan, Hua Yu, Wen-Juan Bao, Hai-Ying Jia, Wei Sci Rep Article A novel polysaccharide FVPB2 was purified from fruiting bodies of Flammulina velutipes. Its structure was elucidated by monosaccharide composition and methylation analyses, UV-Visible and FTIR spectroscopy as well as NMR. FVPB2 was a homogeneous heteropolysaccharide (molecular weight ~ 1.50 × 10(4) Da) containing D-galactose, D-mannose, L-fucose, and D-glucose at molar ratio of 1.9:1.2:1:2.5. In vitro immunomodulatory studies showed FVPB2 induced proliferation of mouse spleen lymphocytes in a dose-dependent manner. The levels of IgM and IgG, secreted by B cells, increased after FVPB2 treatment. So FVPB2 has potential to be a new important immunomodulatory nutraceutical. Nature Publishing Group UK 2018-02-15 /pmc/articles/PMC5814432/ /pubmed/29449636 http://dx.doi.org/10.1038/s41598-018-21375-0 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Wang, Wen-Han
Zhang, Jing-Song
Feng, Ting
Deng, Jing
Lin, Chi-Chung
Fan, Hua
Yu, Wen-Juan
Bao, Hai-Ying
Jia, Wei
Structural elucidation of a polysaccharide from Flammulina velutipes and its immunomodulation activities on mouse B lymphocytes
title Structural elucidation of a polysaccharide from Flammulina velutipes and its immunomodulation activities on mouse B lymphocytes
title_full Structural elucidation of a polysaccharide from Flammulina velutipes and its immunomodulation activities on mouse B lymphocytes
title_fullStr Structural elucidation of a polysaccharide from Flammulina velutipes and its immunomodulation activities on mouse B lymphocytes
title_full_unstemmed Structural elucidation of a polysaccharide from Flammulina velutipes and its immunomodulation activities on mouse B lymphocytes
title_short Structural elucidation of a polysaccharide from Flammulina velutipes and its immunomodulation activities on mouse B lymphocytes
title_sort structural elucidation of a polysaccharide from flammulina velutipes and its immunomodulation activities on mouse b lymphocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5814432/
https://www.ncbi.nlm.nih.gov/pubmed/29449636
http://dx.doi.org/10.1038/s41598-018-21375-0
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