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Stromule extension along microtubules coordinated with actin-mediated anchoring guides perinuclear chloroplast movement during innate immunity

Dynamic tubular extensions from chloroplasts called stromules have recently been shown to connect with nuclei and function during innate immunity. We demonstrate that stromules extend along microtubules (MTs) and MT organization directly affects stromule dynamics since stabilization of MTs chemicall...

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Autores principales: Kumar, Amutha Sampath, Park, Eunsook, Nedo, Alexander, Alqarni, Ali, Ren, Li, Hoban, Kyle, Modla, Shannon, McDonald, John H, Kambhamettu, Chandra, Dinesh-Kumar, Savithramma P, Caplan, Jeffrey Lewis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5815851/
https://www.ncbi.nlm.nih.gov/pubmed/29338837
http://dx.doi.org/10.7554/eLife.23625
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author Kumar, Amutha Sampath
Park, Eunsook
Nedo, Alexander
Alqarni, Ali
Ren, Li
Hoban, Kyle
Modla, Shannon
McDonald, John H
Kambhamettu, Chandra
Dinesh-Kumar, Savithramma P
Caplan, Jeffrey Lewis
author_facet Kumar, Amutha Sampath
Park, Eunsook
Nedo, Alexander
Alqarni, Ali
Ren, Li
Hoban, Kyle
Modla, Shannon
McDonald, John H
Kambhamettu, Chandra
Dinesh-Kumar, Savithramma P
Caplan, Jeffrey Lewis
author_sort Kumar, Amutha Sampath
collection PubMed
description Dynamic tubular extensions from chloroplasts called stromules have recently been shown to connect with nuclei and function during innate immunity. We demonstrate that stromules extend along microtubules (MTs) and MT organization directly affects stromule dynamics since stabilization of MTs chemically or genetically increases stromule numbers and length. Although actin filaments (AFs) are not required for stromule extension, they provide anchor points for stromules. Interestingly, there is a strong correlation between the direction of stromules from chloroplasts and the direction of chloroplast movement. Stromule-directed chloroplast movement was observed in steady-state conditions without immune induction, suggesting it is a general function of stromules in epidermal cells. Our results show that MTs and AFs may facilitate perinuclear clustering of chloroplasts during an innate immune response. We propose a model in which stromules extend along MTs and connect to AF anchor points surrounding nuclei, facilitating stromule-directed movement of chloroplasts to nuclei during innate immunity.
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spelling pubmed-58158512018-02-22 Stromule extension along microtubules coordinated with actin-mediated anchoring guides perinuclear chloroplast movement during innate immunity Kumar, Amutha Sampath Park, Eunsook Nedo, Alexander Alqarni, Ali Ren, Li Hoban, Kyle Modla, Shannon McDonald, John H Kambhamettu, Chandra Dinesh-Kumar, Savithramma P Caplan, Jeffrey Lewis eLife Cell Biology Dynamic tubular extensions from chloroplasts called stromules have recently been shown to connect with nuclei and function during innate immunity. We demonstrate that stromules extend along microtubules (MTs) and MT organization directly affects stromule dynamics since stabilization of MTs chemically or genetically increases stromule numbers and length. Although actin filaments (AFs) are not required for stromule extension, they provide anchor points for stromules. Interestingly, there is a strong correlation between the direction of stromules from chloroplasts and the direction of chloroplast movement. Stromule-directed chloroplast movement was observed in steady-state conditions without immune induction, suggesting it is a general function of stromules in epidermal cells. Our results show that MTs and AFs may facilitate perinuclear clustering of chloroplasts during an innate immune response. We propose a model in which stromules extend along MTs and connect to AF anchor points surrounding nuclei, facilitating stromule-directed movement of chloroplasts to nuclei during innate immunity. eLife Sciences Publications, Ltd 2018-01-17 /pmc/articles/PMC5815851/ /pubmed/29338837 http://dx.doi.org/10.7554/eLife.23625 Text en © 2018, Kumar et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Cell Biology
Kumar, Amutha Sampath
Park, Eunsook
Nedo, Alexander
Alqarni, Ali
Ren, Li
Hoban, Kyle
Modla, Shannon
McDonald, John H
Kambhamettu, Chandra
Dinesh-Kumar, Savithramma P
Caplan, Jeffrey Lewis
Stromule extension along microtubules coordinated with actin-mediated anchoring guides perinuclear chloroplast movement during innate immunity
title Stromule extension along microtubules coordinated with actin-mediated anchoring guides perinuclear chloroplast movement during innate immunity
title_full Stromule extension along microtubules coordinated with actin-mediated anchoring guides perinuclear chloroplast movement during innate immunity
title_fullStr Stromule extension along microtubules coordinated with actin-mediated anchoring guides perinuclear chloroplast movement during innate immunity
title_full_unstemmed Stromule extension along microtubules coordinated with actin-mediated anchoring guides perinuclear chloroplast movement during innate immunity
title_short Stromule extension along microtubules coordinated with actin-mediated anchoring guides perinuclear chloroplast movement during innate immunity
title_sort stromule extension along microtubules coordinated with actin-mediated anchoring guides perinuclear chloroplast movement during innate immunity
topic Cell Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5815851/
https://www.ncbi.nlm.nih.gov/pubmed/29338837
http://dx.doi.org/10.7554/eLife.23625
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