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Stromule extension along microtubules coordinated with actin-mediated anchoring guides perinuclear chloroplast movement during innate immunity
Dynamic tubular extensions from chloroplasts called stromules have recently been shown to connect with nuclei and function during innate immunity. We demonstrate that stromules extend along microtubules (MTs) and MT organization directly affects stromule dynamics since stabilization of MTs chemicall...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5815851/ https://www.ncbi.nlm.nih.gov/pubmed/29338837 http://dx.doi.org/10.7554/eLife.23625 |
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author | Kumar, Amutha Sampath Park, Eunsook Nedo, Alexander Alqarni, Ali Ren, Li Hoban, Kyle Modla, Shannon McDonald, John H Kambhamettu, Chandra Dinesh-Kumar, Savithramma P Caplan, Jeffrey Lewis |
author_facet | Kumar, Amutha Sampath Park, Eunsook Nedo, Alexander Alqarni, Ali Ren, Li Hoban, Kyle Modla, Shannon McDonald, John H Kambhamettu, Chandra Dinesh-Kumar, Savithramma P Caplan, Jeffrey Lewis |
author_sort | Kumar, Amutha Sampath |
collection | PubMed |
description | Dynamic tubular extensions from chloroplasts called stromules have recently been shown to connect with nuclei and function during innate immunity. We demonstrate that stromules extend along microtubules (MTs) and MT organization directly affects stromule dynamics since stabilization of MTs chemically or genetically increases stromule numbers and length. Although actin filaments (AFs) are not required for stromule extension, they provide anchor points for stromules. Interestingly, there is a strong correlation between the direction of stromules from chloroplasts and the direction of chloroplast movement. Stromule-directed chloroplast movement was observed in steady-state conditions without immune induction, suggesting it is a general function of stromules in epidermal cells. Our results show that MTs and AFs may facilitate perinuclear clustering of chloroplasts during an innate immune response. We propose a model in which stromules extend along MTs and connect to AF anchor points surrounding nuclei, facilitating stromule-directed movement of chloroplasts to nuclei during innate immunity. |
format | Online Article Text |
id | pubmed-5815851 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-58158512018-02-22 Stromule extension along microtubules coordinated with actin-mediated anchoring guides perinuclear chloroplast movement during innate immunity Kumar, Amutha Sampath Park, Eunsook Nedo, Alexander Alqarni, Ali Ren, Li Hoban, Kyle Modla, Shannon McDonald, John H Kambhamettu, Chandra Dinesh-Kumar, Savithramma P Caplan, Jeffrey Lewis eLife Cell Biology Dynamic tubular extensions from chloroplasts called stromules have recently been shown to connect with nuclei and function during innate immunity. We demonstrate that stromules extend along microtubules (MTs) and MT organization directly affects stromule dynamics since stabilization of MTs chemically or genetically increases stromule numbers and length. Although actin filaments (AFs) are not required for stromule extension, they provide anchor points for stromules. Interestingly, there is a strong correlation between the direction of stromules from chloroplasts and the direction of chloroplast movement. Stromule-directed chloroplast movement was observed in steady-state conditions without immune induction, suggesting it is a general function of stromules in epidermal cells. Our results show that MTs and AFs may facilitate perinuclear clustering of chloroplasts during an innate immune response. We propose a model in which stromules extend along MTs and connect to AF anchor points surrounding nuclei, facilitating stromule-directed movement of chloroplasts to nuclei during innate immunity. eLife Sciences Publications, Ltd 2018-01-17 /pmc/articles/PMC5815851/ /pubmed/29338837 http://dx.doi.org/10.7554/eLife.23625 Text en © 2018, Kumar et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Cell Biology Kumar, Amutha Sampath Park, Eunsook Nedo, Alexander Alqarni, Ali Ren, Li Hoban, Kyle Modla, Shannon McDonald, John H Kambhamettu, Chandra Dinesh-Kumar, Savithramma P Caplan, Jeffrey Lewis Stromule extension along microtubules coordinated with actin-mediated anchoring guides perinuclear chloroplast movement during innate immunity |
title | Stromule extension along microtubules coordinated with actin-mediated anchoring guides perinuclear chloroplast movement during innate immunity |
title_full | Stromule extension along microtubules coordinated with actin-mediated anchoring guides perinuclear chloroplast movement during innate immunity |
title_fullStr | Stromule extension along microtubules coordinated with actin-mediated anchoring guides perinuclear chloroplast movement during innate immunity |
title_full_unstemmed | Stromule extension along microtubules coordinated with actin-mediated anchoring guides perinuclear chloroplast movement during innate immunity |
title_short | Stromule extension along microtubules coordinated with actin-mediated anchoring guides perinuclear chloroplast movement during innate immunity |
title_sort | stromule extension along microtubules coordinated with actin-mediated anchoring guides perinuclear chloroplast movement during innate immunity |
topic | Cell Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5815851/ https://www.ncbi.nlm.nih.gov/pubmed/29338837 http://dx.doi.org/10.7554/eLife.23625 |
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