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Hepatitis B Virus-Specific CD8+ T Cells Maintain Functional Exhaustion after Antigen Reexposure in an Acute Activation Immune Environment
Chronic hepatitis B virus (HBV) infection is characterized by the presence of functionally exhausted HBV-specific CD8+ T cells. To characterize the possible residual effector ability of these cells, we reexposed CD8+ T cells from chronically HBV replicating mice to HBV antigens in an acute activatio...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5816053/ https://www.ncbi.nlm.nih.gov/pubmed/29483916 http://dx.doi.org/10.3389/fimmu.2018.00219 |
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author | Wang, Qin Pan, Wen Liu, Yanan Luo, Jinzhuo Zhu, Dan Lu, Yinping Feng, Xuemei Yang, Xuecheng Dittmer, Ulf Lu, Mengji Yang, Dongliang Liu, Jia |
author_facet | Wang, Qin Pan, Wen Liu, Yanan Luo, Jinzhuo Zhu, Dan Lu, Yinping Feng, Xuemei Yang, Xuecheng Dittmer, Ulf Lu, Mengji Yang, Dongliang Liu, Jia |
author_sort | Wang, Qin |
collection | PubMed |
description | Chronic hepatitis B virus (HBV) infection is characterized by the presence of functionally exhausted HBV-specific CD8+ T cells. To characterize the possible residual effector ability of these cells, we reexposed CD8+ T cells from chronically HBV replicating mice to HBV antigens in an acute activation immune environment. We found that after transfer into naive mice, exhausted CD8+ T cells reexpanded in a comparable magnitude as naive CD8+ T cells in response to acute HBV infection; however, their proliferation intensity was significantly lower than that of CD8+ T cells from acute-resolving HBV replicating mice (AR mice). The differentiation phenotypes driven by acute HBV replication of donor exhausted and naive CD8+ T cells were similar, but were different from those of their counterparts from AR mice. Nevertheless, exhausted CD8+ T cells maintained less activated phenotype, an absence of effector cytokine production and poor antiviral function after HBV reexposure in an acute activation immune environment. We thus conclude that exhausted CD8+ T cells undergo a stable form of dysfunctional differentiation during chronic HBV replication and switching immune environment alone is not sufficient for the antiviral functional reconstitution of these cells. |
format | Online Article Text |
id | pubmed-5816053 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-58160532018-02-26 Hepatitis B Virus-Specific CD8+ T Cells Maintain Functional Exhaustion after Antigen Reexposure in an Acute Activation Immune Environment Wang, Qin Pan, Wen Liu, Yanan Luo, Jinzhuo Zhu, Dan Lu, Yinping Feng, Xuemei Yang, Xuecheng Dittmer, Ulf Lu, Mengji Yang, Dongliang Liu, Jia Front Immunol Immunology Chronic hepatitis B virus (HBV) infection is characterized by the presence of functionally exhausted HBV-specific CD8+ T cells. To characterize the possible residual effector ability of these cells, we reexposed CD8+ T cells from chronically HBV replicating mice to HBV antigens in an acute activation immune environment. We found that after transfer into naive mice, exhausted CD8+ T cells reexpanded in a comparable magnitude as naive CD8+ T cells in response to acute HBV infection; however, their proliferation intensity was significantly lower than that of CD8+ T cells from acute-resolving HBV replicating mice (AR mice). The differentiation phenotypes driven by acute HBV replication of donor exhausted and naive CD8+ T cells were similar, but were different from those of their counterparts from AR mice. Nevertheless, exhausted CD8+ T cells maintained less activated phenotype, an absence of effector cytokine production and poor antiviral function after HBV reexposure in an acute activation immune environment. We thus conclude that exhausted CD8+ T cells undergo a stable form of dysfunctional differentiation during chronic HBV replication and switching immune environment alone is not sufficient for the antiviral functional reconstitution of these cells. Frontiers Media S.A. 2018-02-12 /pmc/articles/PMC5816053/ /pubmed/29483916 http://dx.doi.org/10.3389/fimmu.2018.00219 Text en Copyright © 2018 Wang, Pan, Liu, Luo, Zhu, Lu, Feng, Yang, Dittmer, Lu, Yang and Liu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Wang, Qin Pan, Wen Liu, Yanan Luo, Jinzhuo Zhu, Dan Lu, Yinping Feng, Xuemei Yang, Xuecheng Dittmer, Ulf Lu, Mengji Yang, Dongliang Liu, Jia Hepatitis B Virus-Specific CD8+ T Cells Maintain Functional Exhaustion after Antigen Reexposure in an Acute Activation Immune Environment |
title | Hepatitis B Virus-Specific CD8+ T Cells Maintain Functional Exhaustion after Antigen Reexposure in an Acute Activation Immune Environment |
title_full | Hepatitis B Virus-Specific CD8+ T Cells Maintain Functional Exhaustion after Antigen Reexposure in an Acute Activation Immune Environment |
title_fullStr | Hepatitis B Virus-Specific CD8+ T Cells Maintain Functional Exhaustion after Antigen Reexposure in an Acute Activation Immune Environment |
title_full_unstemmed | Hepatitis B Virus-Specific CD8+ T Cells Maintain Functional Exhaustion after Antigen Reexposure in an Acute Activation Immune Environment |
title_short | Hepatitis B Virus-Specific CD8+ T Cells Maintain Functional Exhaustion after Antigen Reexposure in an Acute Activation Immune Environment |
title_sort | hepatitis b virus-specific cd8+ t cells maintain functional exhaustion after antigen reexposure in an acute activation immune environment |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5816053/ https://www.ncbi.nlm.nih.gov/pubmed/29483916 http://dx.doi.org/10.3389/fimmu.2018.00219 |
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