Role of intestinal trefoil factor in protecting intestinal epithelial cells from burn-induced injury

Although intestinal trefoil factor (ITF) can alleviate the burn-induced intestinal mucosa injury, the underlying mechanisms remains elusive. In this study, we investigated if ITF alters glutamine transport on the brush border membrane vesicles (BBMVs) of the intestines in Sprague-Dawley rats inflict...

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Autores principales: Hu, Jianhong, Shi, Yan, Wang, Chao, Wan, Hanxing, Wu, Dan, Wang, Hongyu, Peng, Xi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5816625/
https://www.ncbi.nlm.nih.gov/pubmed/29453360
http://dx.doi.org/10.1038/s41598-018-21282-4
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author Hu, Jianhong
Shi, Yan
Wang, Chao
Wan, Hanxing
Wu, Dan
Wang, Hongyu
Peng, Xi
author_facet Hu, Jianhong
Shi, Yan
Wang, Chao
Wan, Hanxing
Wu, Dan
Wang, Hongyu
Peng, Xi
author_sort Hu, Jianhong
collection PubMed
description Although intestinal trefoil factor (ITF) can alleviate the burn-induced intestinal mucosa injury, the underlying mechanisms remains elusive. In this study, we investigated if ITF alters glutamine transport on the brush border membrane vesicles (BBMVs) of the intestines in Sprague-Dawley rats inflicted with 30% TBSA and the underlying mechanisms. We found that ITF significantly stimulated intestinal glutamine transport in burned rats. Mechanistically, ITF enhanced autophagy, reduces endoplasmic reticulum stress (ERS), and alleviates the impaired PDI, ASCT2, and B0AT1 in IECs and BBMVs after burn injury likely through AMPK activation. Therefore, ITF may protect intestinal epithelial cells from burn-induced injury through improving glutamine transport by alleviating ERS.
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spelling pubmed-58166252018-02-21 Role of intestinal trefoil factor in protecting intestinal epithelial cells from burn-induced injury Hu, Jianhong Shi, Yan Wang, Chao Wan, Hanxing Wu, Dan Wang, Hongyu Peng, Xi Sci Rep Article Although intestinal trefoil factor (ITF) can alleviate the burn-induced intestinal mucosa injury, the underlying mechanisms remains elusive. In this study, we investigated if ITF alters glutamine transport on the brush border membrane vesicles (BBMVs) of the intestines in Sprague-Dawley rats inflicted with 30% TBSA and the underlying mechanisms. We found that ITF significantly stimulated intestinal glutamine transport in burned rats. Mechanistically, ITF enhanced autophagy, reduces endoplasmic reticulum stress (ERS), and alleviates the impaired PDI, ASCT2, and B0AT1 in IECs and BBMVs after burn injury likely through AMPK activation. Therefore, ITF may protect intestinal epithelial cells from burn-induced injury through improving glutamine transport by alleviating ERS. Nature Publishing Group UK 2018-02-16 /pmc/articles/PMC5816625/ /pubmed/29453360 http://dx.doi.org/10.1038/s41598-018-21282-4 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Hu, Jianhong
Shi, Yan
Wang, Chao
Wan, Hanxing
Wu, Dan
Wang, Hongyu
Peng, Xi
Role of intestinal trefoil factor in protecting intestinal epithelial cells from burn-induced injury
title Role of intestinal trefoil factor in protecting intestinal epithelial cells from burn-induced injury
title_full Role of intestinal trefoil factor in protecting intestinal epithelial cells from burn-induced injury
title_fullStr Role of intestinal trefoil factor in protecting intestinal epithelial cells from burn-induced injury
title_full_unstemmed Role of intestinal trefoil factor in protecting intestinal epithelial cells from burn-induced injury
title_short Role of intestinal trefoil factor in protecting intestinal epithelial cells from burn-induced injury
title_sort role of intestinal trefoil factor in protecting intestinal epithelial cells from burn-induced injury
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5816625/
https://www.ncbi.nlm.nih.gov/pubmed/29453360
http://dx.doi.org/10.1038/s41598-018-21282-4
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