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A multiplex serologic platform for diagnosis of tick-borne diseases

Tick-borne diseases are the most common vector-borne diseases in the United States, with serology being the primary method of diagnosis. We developed the first multiplex, array-based assay for serodiagnosis of tick-borne diseases called the TBD-Serochip. The TBD-Serochip was designed to discriminate...

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Autores principales: Tokarz, Rafal, Mishra, Nischay, Tagliafierro, Teresa, Sameroff, Stephen, Caciula, Adrian, Chauhan, Lokendrasingh, Patel, Jigar, Sullivan, Eric, Gucwa, Azad, Fallon, Brian, Golightly, Marc, Molins, Claudia, Schriefer, Martin, Marques, Adriana, Briese, Thomas, Lipkin, W. Ian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5816631/
https://www.ncbi.nlm.nih.gov/pubmed/29453420
http://dx.doi.org/10.1038/s41598-018-21349-2
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author Tokarz, Rafal
Mishra, Nischay
Tagliafierro, Teresa
Sameroff, Stephen
Caciula, Adrian
Chauhan, Lokendrasingh
Patel, Jigar
Sullivan, Eric
Gucwa, Azad
Fallon, Brian
Golightly, Marc
Molins, Claudia
Schriefer, Martin
Marques, Adriana
Briese, Thomas
Lipkin, W. Ian
author_facet Tokarz, Rafal
Mishra, Nischay
Tagliafierro, Teresa
Sameroff, Stephen
Caciula, Adrian
Chauhan, Lokendrasingh
Patel, Jigar
Sullivan, Eric
Gucwa, Azad
Fallon, Brian
Golightly, Marc
Molins, Claudia
Schriefer, Martin
Marques, Adriana
Briese, Thomas
Lipkin, W. Ian
author_sort Tokarz, Rafal
collection PubMed
description Tick-borne diseases are the most common vector-borne diseases in the United States, with serology being the primary method of diagnosis. We developed the first multiplex, array-based assay for serodiagnosis of tick-borne diseases called the TBD-Serochip. The TBD-Serochip was designed to discriminate antibody responses to 8 major tick-borne pathogens present in the United States, including Anaplasma phagocytophilum, Babesia microti, Borrelia burgdorferi, Borrelia miyamotoi, Ehrlichia chaffeensis, Rickettsia rickettsii, Heartland virus and Powassan virus. Each assay contains approximately 170,000 12-mer linear peptides that tile along the protein sequence of the major antigens from each agent with 11 amino acid overlap. This permits accurate identification of a wide range of specific immunodominant IgG and IgM epitopes that can then be used to enhance diagnostic accuracy and integrate differential diagnosis into a single assay. To test the performance of the TBD-Serochip, we examined sera from patients with confirmed Lyme disease, babesiosis, anaplasmosis, and Powassan virus disease. We identified a wide range of specific discriminatory epitopes that facilitated accurate diagnosis of each disease. We also identified previously undiagnosed infections. Our results indicate that the TBD-Serochip is a promising tool for a differential diagnosis not available with currently employed serologic assays for TBDs.
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spelling pubmed-58166312018-02-21 A multiplex serologic platform for diagnosis of tick-borne diseases Tokarz, Rafal Mishra, Nischay Tagliafierro, Teresa Sameroff, Stephen Caciula, Adrian Chauhan, Lokendrasingh Patel, Jigar Sullivan, Eric Gucwa, Azad Fallon, Brian Golightly, Marc Molins, Claudia Schriefer, Martin Marques, Adriana Briese, Thomas Lipkin, W. Ian Sci Rep Article Tick-borne diseases are the most common vector-borne diseases in the United States, with serology being the primary method of diagnosis. We developed the first multiplex, array-based assay for serodiagnosis of tick-borne diseases called the TBD-Serochip. The TBD-Serochip was designed to discriminate antibody responses to 8 major tick-borne pathogens present in the United States, including Anaplasma phagocytophilum, Babesia microti, Borrelia burgdorferi, Borrelia miyamotoi, Ehrlichia chaffeensis, Rickettsia rickettsii, Heartland virus and Powassan virus. Each assay contains approximately 170,000 12-mer linear peptides that tile along the protein sequence of the major antigens from each agent with 11 amino acid overlap. This permits accurate identification of a wide range of specific immunodominant IgG and IgM epitopes that can then be used to enhance diagnostic accuracy and integrate differential diagnosis into a single assay. To test the performance of the TBD-Serochip, we examined sera from patients with confirmed Lyme disease, babesiosis, anaplasmosis, and Powassan virus disease. We identified a wide range of specific discriminatory epitopes that facilitated accurate diagnosis of each disease. We also identified previously undiagnosed infections. Our results indicate that the TBD-Serochip is a promising tool for a differential diagnosis not available with currently employed serologic assays for TBDs. Nature Publishing Group UK 2018-02-16 /pmc/articles/PMC5816631/ /pubmed/29453420 http://dx.doi.org/10.1038/s41598-018-21349-2 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Tokarz, Rafal
Mishra, Nischay
Tagliafierro, Teresa
Sameroff, Stephen
Caciula, Adrian
Chauhan, Lokendrasingh
Patel, Jigar
Sullivan, Eric
Gucwa, Azad
Fallon, Brian
Golightly, Marc
Molins, Claudia
Schriefer, Martin
Marques, Adriana
Briese, Thomas
Lipkin, W. Ian
A multiplex serologic platform for diagnosis of tick-borne diseases
title A multiplex serologic platform for diagnosis of tick-borne diseases
title_full A multiplex serologic platform for diagnosis of tick-borne diseases
title_fullStr A multiplex serologic platform for diagnosis of tick-borne diseases
title_full_unstemmed A multiplex serologic platform for diagnosis of tick-borne diseases
title_short A multiplex serologic platform for diagnosis of tick-borne diseases
title_sort multiplex serologic platform for diagnosis of tick-borne diseases
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5816631/
https://www.ncbi.nlm.nih.gov/pubmed/29453420
http://dx.doi.org/10.1038/s41598-018-21349-2
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