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Influence of Adalimumab on the Expression Profile of Genes Associated with the Histaminergic System in the Skin Fibroblasts In Vitro
OBJECTIVE: The aim of this study was to evaluate the influence of adalimumab on expression profile of genes associated with the histaminergic system in Normal Human Dermal Fibroblast (NHDF) cells stimulated with 8.00 μg/ml of adalimumab and the identification of miRNAs regulating these genes' e...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5816894/ https://www.ncbi.nlm.nih.gov/pubmed/29487864 http://dx.doi.org/10.1155/2018/1582173 |
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author | Wcisło-Dziadecka, Dominika Grabarek, Beniamin Zmarzły, Nikola Skubis, Aleksandra Sikora, Bartosz Kruszniewska-Rajs, Celina Gola, Joanna Mazurek, Urszula Kucharz, Eugeniusz |
author_facet | Wcisło-Dziadecka, Dominika Grabarek, Beniamin Zmarzły, Nikola Skubis, Aleksandra Sikora, Bartosz Kruszniewska-Rajs, Celina Gola, Joanna Mazurek, Urszula Kucharz, Eugeniusz |
author_sort | Wcisło-Dziadecka, Dominika |
collection | PubMed |
description | OBJECTIVE: The aim of this study was to evaluate the influence of adalimumab on expression profile of genes associated with the histaminergic system in Normal Human Dermal Fibroblast (NHDF) cells stimulated with 8.00 μg/ml of adalimumab and the identification of miRNAs regulating these genes' expression. METHODS: NHDFs were cultured with or without the presence of adalimumab for 2, 8, and 24 hours. The expression profile of genes and miRNA were determined with the use of microarray technology. RESULTS: Among 22283 ID mRNA, 65 are associated with the histaminergic system. It can be observed that 15 mRNAs differentiate NHDFs cultures with adalimumab form control. The analysis of miRNAs showed that, among 1105 ID miRNA, 20 miRNAs are differentiating in cells treated with adalimumab for 2 hours, 9 miRNA after 8 hours, and only 3 miRNAs after 24 hours. CONCLUSION: It was also determined that miRNAs play certain role in the regulation of the expression of genes associated with the histaminergic system. The results of this study confirmed the possibility of using both genes associated with this system as well as miRNAs regulating their expression, as complementary molecular markers of sensitivity to the adalimumab treatment. |
format | Online Article Text |
id | pubmed-5816894 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-58168942018-02-27 Influence of Adalimumab on the Expression Profile of Genes Associated with the Histaminergic System in the Skin Fibroblasts In Vitro Wcisło-Dziadecka, Dominika Grabarek, Beniamin Zmarzły, Nikola Skubis, Aleksandra Sikora, Bartosz Kruszniewska-Rajs, Celina Gola, Joanna Mazurek, Urszula Kucharz, Eugeniusz Biomed Res Int Research Article OBJECTIVE: The aim of this study was to evaluate the influence of adalimumab on expression profile of genes associated with the histaminergic system in Normal Human Dermal Fibroblast (NHDF) cells stimulated with 8.00 μg/ml of adalimumab and the identification of miRNAs regulating these genes' expression. METHODS: NHDFs were cultured with or without the presence of adalimumab for 2, 8, and 24 hours. The expression profile of genes and miRNA were determined with the use of microarray technology. RESULTS: Among 22283 ID mRNA, 65 are associated with the histaminergic system. It can be observed that 15 mRNAs differentiate NHDFs cultures with adalimumab form control. The analysis of miRNAs showed that, among 1105 ID miRNA, 20 miRNAs are differentiating in cells treated with adalimumab for 2 hours, 9 miRNA after 8 hours, and only 3 miRNAs after 24 hours. CONCLUSION: It was also determined that miRNAs play certain role in the regulation of the expression of genes associated with the histaminergic system. The results of this study confirmed the possibility of using both genes associated with this system as well as miRNAs regulating their expression, as complementary molecular markers of sensitivity to the adalimumab treatment. Hindawi 2018-01-02 /pmc/articles/PMC5816894/ /pubmed/29487864 http://dx.doi.org/10.1155/2018/1582173 Text en Copyright © 2018 Dominika Wcisło-Dziadecka et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Wcisło-Dziadecka, Dominika Grabarek, Beniamin Zmarzły, Nikola Skubis, Aleksandra Sikora, Bartosz Kruszniewska-Rajs, Celina Gola, Joanna Mazurek, Urszula Kucharz, Eugeniusz Influence of Adalimumab on the Expression Profile of Genes Associated with the Histaminergic System in the Skin Fibroblasts In Vitro |
title | Influence of Adalimumab on the Expression Profile of Genes Associated with the Histaminergic System in the Skin Fibroblasts In Vitro |
title_full | Influence of Adalimumab on the Expression Profile of Genes Associated with the Histaminergic System in the Skin Fibroblasts In Vitro |
title_fullStr | Influence of Adalimumab on the Expression Profile of Genes Associated with the Histaminergic System in the Skin Fibroblasts In Vitro |
title_full_unstemmed | Influence of Adalimumab on the Expression Profile of Genes Associated with the Histaminergic System in the Skin Fibroblasts In Vitro |
title_short | Influence of Adalimumab on the Expression Profile of Genes Associated with the Histaminergic System in the Skin Fibroblasts In Vitro |
title_sort | influence of adalimumab on the expression profile of genes associated with the histaminergic system in the skin fibroblasts in vitro |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5816894/ https://www.ncbi.nlm.nih.gov/pubmed/29487864 http://dx.doi.org/10.1155/2018/1582173 |
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