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Dehydrins Impart Protection against Oxidative Stress in Transgenic Tobacco Plants

Environmental stresses generate reactive oxygen species (ROS) which might be detrimental to the plants when produced in an uncontrolled way. However, the plants ameliorate such stresses by synthesizing antioxidants and enzymes responsible for the dismutation of ROS. Additionally, the dehydrins were...

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Autores principales: Halder, Tanmoy, Upadhyaya, Gouranga, Basak, Chandra, Das, Arup, Chakraborty, Chandrima, Ray, Sudipta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5817096/
https://www.ncbi.nlm.nih.gov/pubmed/29491874
http://dx.doi.org/10.3389/fpls.2018.00136
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author Halder, Tanmoy
Upadhyaya, Gouranga
Basak, Chandra
Das, Arup
Chakraborty, Chandrima
Ray, Sudipta
author_facet Halder, Tanmoy
Upadhyaya, Gouranga
Basak, Chandra
Das, Arup
Chakraborty, Chandrima
Ray, Sudipta
author_sort Halder, Tanmoy
collection PubMed
description Environmental stresses generate reactive oxygen species (ROS) which might be detrimental to the plants when produced in an uncontrolled way. However, the plants ameliorate such stresses by synthesizing antioxidants and enzymes responsible for the dismutation of ROS. Additionally, the dehydrins were also able to protect the inactivation of the enzyme lactate dehydrogenase against hydroxyl radicals (OH(⋅)) generated during Fenton’s reaction. SbDhn1 and SbDhn2 overexpressing transgenic tobacco plants were able to protect against oxidative damage. Transgenic tobacco lines showed better photosynthetic efficiency along with high chlorophyll content, soluble sugar and proline. However, the malonyl dialdehyde (MDA) content was significantly lower in transgenic lines. Experimental evidence demonstrates the protective effect of dehydrins on electron transport chain in isolated chloroplast upon methyl viologen (MV) treatment. The transgenic tobacco plants showed significantly lower superoxide radical generation ([Image: see text]) upon MV treatment. The accumulation of the H(2)O(2) was also lower in the transgenic plants. Furthermore, in the transgenic plants the expression of ROS scavenging enzymes was higher compared to non-transformed (NT) or vector transformed (VT) plants. Taken together these data, during oxidative stress dehydrins function by scavenging the ([Image: see text]) directly and also by rendering protection to the enzymes responsible for the dismutation of ([Image: see text]) thereby significantly reducing the amount of hydrogen peroxides formed. Increase in proline content along with other antioxidants might also play a significant role in stress amelioration. Dehydrins thus function co-operatively with other protective mechanisms under oxidative stress conditions rendering protection in stress environment.
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spelling pubmed-58170962018-02-28 Dehydrins Impart Protection against Oxidative Stress in Transgenic Tobacco Plants Halder, Tanmoy Upadhyaya, Gouranga Basak, Chandra Das, Arup Chakraborty, Chandrima Ray, Sudipta Front Plant Sci Plant Science Environmental stresses generate reactive oxygen species (ROS) which might be detrimental to the plants when produced in an uncontrolled way. However, the plants ameliorate such stresses by synthesizing antioxidants and enzymes responsible for the dismutation of ROS. Additionally, the dehydrins were also able to protect the inactivation of the enzyme lactate dehydrogenase against hydroxyl radicals (OH(⋅)) generated during Fenton’s reaction. SbDhn1 and SbDhn2 overexpressing transgenic tobacco plants were able to protect against oxidative damage. Transgenic tobacco lines showed better photosynthetic efficiency along with high chlorophyll content, soluble sugar and proline. However, the malonyl dialdehyde (MDA) content was significantly lower in transgenic lines. Experimental evidence demonstrates the protective effect of dehydrins on electron transport chain in isolated chloroplast upon methyl viologen (MV) treatment. The transgenic tobacco plants showed significantly lower superoxide radical generation ([Image: see text]) upon MV treatment. The accumulation of the H(2)O(2) was also lower in the transgenic plants. Furthermore, in the transgenic plants the expression of ROS scavenging enzymes was higher compared to non-transformed (NT) or vector transformed (VT) plants. Taken together these data, during oxidative stress dehydrins function by scavenging the ([Image: see text]) directly and also by rendering protection to the enzymes responsible for the dismutation of ([Image: see text]) thereby significantly reducing the amount of hydrogen peroxides formed. Increase in proline content along with other antioxidants might also play a significant role in stress amelioration. Dehydrins thus function co-operatively with other protective mechanisms under oxidative stress conditions rendering protection in stress environment. Frontiers Media S.A. 2018-02-14 /pmc/articles/PMC5817096/ /pubmed/29491874 http://dx.doi.org/10.3389/fpls.2018.00136 Text en Copyright © 2018 Halder, Upadhyaya, Basak, Das, Chakraborty and Ray. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Halder, Tanmoy
Upadhyaya, Gouranga
Basak, Chandra
Das, Arup
Chakraborty, Chandrima
Ray, Sudipta
Dehydrins Impart Protection against Oxidative Stress in Transgenic Tobacco Plants
title Dehydrins Impart Protection against Oxidative Stress in Transgenic Tobacco Plants
title_full Dehydrins Impart Protection against Oxidative Stress in Transgenic Tobacco Plants
title_fullStr Dehydrins Impart Protection against Oxidative Stress in Transgenic Tobacco Plants
title_full_unstemmed Dehydrins Impart Protection against Oxidative Stress in Transgenic Tobacco Plants
title_short Dehydrins Impart Protection against Oxidative Stress in Transgenic Tobacco Plants
title_sort dehydrins impart protection against oxidative stress in transgenic tobacco plants
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5817096/
https://www.ncbi.nlm.nih.gov/pubmed/29491874
http://dx.doi.org/10.3389/fpls.2018.00136
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