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Systematic Analysis of RNA Regulatory Network in Rat Brain after Ischemic Stroke
Although extensive studies have identified large number of microRNAs (miRNAs) and long noncoding RNAs (lncRNAs) in ischemic stroke, the RNA regulation network response to focal ischemia remains poorly understood. In this study, we simultaneously interrogate the expression profiles of lncRNAs, miRNAs...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5817225/ https://www.ncbi.nlm.nih.gov/pubmed/29516010 http://dx.doi.org/10.1155/2018/8354350 |
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author | Liu, Juan Zhang, Kun-shan Hu, Bin Li, Si-guang Li, Qing Luo, Yu-ping Wang, Yang Deng, Zhi-Feng |
author_facet | Liu, Juan Zhang, Kun-shan Hu, Bin Li, Si-guang Li, Qing Luo, Yu-ping Wang, Yang Deng, Zhi-Feng |
author_sort | Liu, Juan |
collection | PubMed |
description | Although extensive studies have identified large number of microRNAs (miRNAs) and long noncoding RNAs (lncRNAs) in ischemic stroke, the RNA regulation network response to focal ischemia remains poorly understood. In this study, we simultaneously interrogate the expression profiles of lncRNAs, miRNAs, and mRNAs changes during focal ischemia induced by transient middle cerebral artery occlusion. A set of 1924 novel lncRNAs were identified and may involve brain injury and DNA repair as revealed by coexpression network analysis. Furthermore, many short interspersed elements (SINE) mediated lncRNA:mRNA duplexes were identified, implying that lncRNAs mediate Staufen1-mediated mRNA decay (SMD) which may play a role during focal ischemia. Moreover, based on the competitive endogenous RNA (ceRNA) hypothesis, a stroke regulatory ceRNA network which reveals functional lncRNA:miRNA:mRNA interactions was revealed in ischemic stroke. In brief, this work reports a large number of novel lncRNAs responding to focal ischemia and constructs a systematic RNA regulation network which highlighted the role of ncRNAs in ischemic stroke. |
format | Online Article Text |
id | pubmed-5817225 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-58172252018-03-07 Systematic Analysis of RNA Regulatory Network in Rat Brain after Ischemic Stroke Liu, Juan Zhang, Kun-shan Hu, Bin Li, Si-guang Li, Qing Luo, Yu-ping Wang, Yang Deng, Zhi-Feng Biomed Res Int Research Article Although extensive studies have identified large number of microRNAs (miRNAs) and long noncoding RNAs (lncRNAs) in ischemic stroke, the RNA regulation network response to focal ischemia remains poorly understood. In this study, we simultaneously interrogate the expression profiles of lncRNAs, miRNAs, and mRNAs changes during focal ischemia induced by transient middle cerebral artery occlusion. A set of 1924 novel lncRNAs were identified and may involve brain injury and DNA repair as revealed by coexpression network analysis. Furthermore, many short interspersed elements (SINE) mediated lncRNA:mRNA duplexes were identified, implying that lncRNAs mediate Staufen1-mediated mRNA decay (SMD) which may play a role during focal ischemia. Moreover, based on the competitive endogenous RNA (ceRNA) hypothesis, a stroke regulatory ceRNA network which reveals functional lncRNA:miRNA:mRNA interactions was revealed in ischemic stroke. In brief, this work reports a large number of novel lncRNAs responding to focal ischemia and constructs a systematic RNA regulation network which highlighted the role of ncRNAs in ischemic stroke. Hindawi 2018-01-08 /pmc/articles/PMC5817225/ /pubmed/29516010 http://dx.doi.org/10.1155/2018/8354350 Text en Copyright © 2018 Juan Liu et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Liu, Juan Zhang, Kun-shan Hu, Bin Li, Si-guang Li, Qing Luo, Yu-ping Wang, Yang Deng, Zhi-Feng Systematic Analysis of RNA Regulatory Network in Rat Brain after Ischemic Stroke |
title | Systematic Analysis of RNA Regulatory Network in Rat Brain after Ischemic Stroke |
title_full | Systematic Analysis of RNA Regulatory Network in Rat Brain after Ischemic Stroke |
title_fullStr | Systematic Analysis of RNA Regulatory Network in Rat Brain after Ischemic Stroke |
title_full_unstemmed | Systematic Analysis of RNA Regulatory Network in Rat Brain after Ischemic Stroke |
title_short | Systematic Analysis of RNA Regulatory Network in Rat Brain after Ischemic Stroke |
title_sort | systematic analysis of rna regulatory network in rat brain after ischemic stroke |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5817225/ https://www.ncbi.nlm.nih.gov/pubmed/29516010 http://dx.doi.org/10.1155/2018/8354350 |
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