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Reproducible diagnosis of chronic lymphocytic leukemia by flow cytometry: An European Research Initiative on CLL (ERIC) & European Society for Clinical Cell Analysis (ESCCA) Harmonisation project
The diagnostic criteria for CLL rely on morphology and immunophenotype. Current approaches have limitations affecting reproducibility and there is no consensus on the role of new markers. The aim of this project was to identify reproducible criteria and consensus on markers recommended for the diagn...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5817234/ https://www.ncbi.nlm.nih.gov/pubmed/29024461 http://dx.doi.org/10.1002/cyto.b.21595 |
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author | Rawstron, Andy C. Kreuzer, Karl‐Anton Soosapilla, Asha Spacek, Martin Stehlikova, Olga Gambell, Peter McIver‐Brown, Neil Villamor, Neus Psarra, Katherina Arroz, Maria Milani, Raffaella de la Serna, Javier Cedena, M. Teresa Jaksic, Ozren Nomdedeu, Josep Moreno, Carol Rigolin, Gian Matteo Cuneo, Antonio Johansen, Preben Johnsen, Hans E. Rosenquist, Richard Niemann, Carsten Utoft Kern, Wolfgang Westerman, David Trneny, Marek Mulligan, Stephen Doubek, Michael Pospisilova, Sarka Hillmen, Peter Oscier, David Hallek, Michael Ghia, Paolo Montserrat, Emili |
author_facet | Rawstron, Andy C. Kreuzer, Karl‐Anton Soosapilla, Asha Spacek, Martin Stehlikova, Olga Gambell, Peter McIver‐Brown, Neil Villamor, Neus Psarra, Katherina Arroz, Maria Milani, Raffaella de la Serna, Javier Cedena, M. Teresa Jaksic, Ozren Nomdedeu, Josep Moreno, Carol Rigolin, Gian Matteo Cuneo, Antonio Johansen, Preben Johnsen, Hans E. Rosenquist, Richard Niemann, Carsten Utoft Kern, Wolfgang Westerman, David Trneny, Marek Mulligan, Stephen Doubek, Michael Pospisilova, Sarka Hillmen, Peter Oscier, David Hallek, Michael Ghia, Paolo Montserrat, Emili |
author_sort | Rawstron, Andy C. |
collection | PubMed |
description | The diagnostic criteria for CLL rely on morphology and immunophenotype. Current approaches have limitations affecting reproducibility and there is no consensus on the role of new markers. The aim of this project was to identify reproducible criteria and consensus on markers recommended for the diagnosis of CLL. ERIC/ESCCA members classified 14 of 35 potential markers as “required” or “recommended” for CLL diagnosis, consensus being defined as >75% and >50% agreement, respectively. An approach to validate “required” markers using normal peripheral blood was developed. Responses were received from 150 participants with a diagnostic workload >20 CLL cases per week in 23/150 (15%), 5–20 in 82/150 (55%), and <5 cases per week in 45/150 (30%). The consensus for “required” diagnostic markers included: CD19, CD5, CD20, CD23, Kappa, and Lambda. “Recommended” markers potentially useful for differential diagnosis were: CD43, CD79b, CD81, CD200, CD10, and ROR1. Reproducible criteria for component reagents were assessed retrospectively in 14,643 cases from 13 different centers and showed >97% concordance with current approaches. A pilot study to validate staining quality was completed in 11 centers. Markers considered as “required” for the diagnosis of CLL by the participants in this study (CD19, CD5, CD20, CD23, Kappa, and Lambda) are consistent with current diagnostic criteria and practice. Importantly, a reproducible approach to validate and apply these markers in individual laboratories has been identified. Finally, a consensus “recommended” panel of markers to refine diagnosis in borderline cases (CD43, CD79b, CD81, CD200, CD10, and ROR1) has been defined and will be prospectively evaluated. © 2017 International Clinical Cytometry Society |
format | Online Article Text |
id | pubmed-5817234 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-58172342018-02-26 Reproducible diagnosis of chronic lymphocytic leukemia by flow cytometry: An European Research Initiative on CLL (ERIC) & European Society for Clinical Cell Analysis (ESCCA) Harmonisation project Rawstron, Andy C. Kreuzer, Karl‐Anton Soosapilla, Asha Spacek, Martin Stehlikova, Olga Gambell, Peter McIver‐Brown, Neil Villamor, Neus Psarra, Katherina Arroz, Maria Milani, Raffaella de la Serna, Javier Cedena, M. Teresa Jaksic, Ozren Nomdedeu, Josep Moreno, Carol Rigolin, Gian Matteo Cuneo, Antonio Johansen, Preben Johnsen, Hans E. Rosenquist, Richard Niemann, Carsten Utoft Kern, Wolfgang Westerman, David Trneny, Marek Mulligan, Stephen Doubek, Michael Pospisilova, Sarka Hillmen, Peter Oscier, David Hallek, Michael Ghia, Paolo Montserrat, Emili Cytometry B Clin Cytom Original Articles The diagnostic criteria for CLL rely on morphology and immunophenotype. Current approaches have limitations affecting reproducibility and there is no consensus on the role of new markers. The aim of this project was to identify reproducible criteria and consensus on markers recommended for the diagnosis of CLL. ERIC/ESCCA members classified 14 of 35 potential markers as “required” or “recommended” for CLL diagnosis, consensus being defined as >75% and >50% agreement, respectively. An approach to validate “required” markers using normal peripheral blood was developed. Responses were received from 150 participants with a diagnostic workload >20 CLL cases per week in 23/150 (15%), 5–20 in 82/150 (55%), and <5 cases per week in 45/150 (30%). The consensus for “required” diagnostic markers included: CD19, CD5, CD20, CD23, Kappa, and Lambda. “Recommended” markers potentially useful for differential diagnosis were: CD43, CD79b, CD81, CD200, CD10, and ROR1. Reproducible criteria for component reagents were assessed retrospectively in 14,643 cases from 13 different centers and showed >97% concordance with current approaches. A pilot study to validate staining quality was completed in 11 centers. Markers considered as “required” for the diagnosis of CLL by the participants in this study (CD19, CD5, CD20, CD23, Kappa, and Lambda) are consistent with current diagnostic criteria and practice. Importantly, a reproducible approach to validate and apply these markers in individual laboratories has been identified. Finally, a consensus “recommended” panel of markers to refine diagnosis in borderline cases (CD43, CD79b, CD81, CD200, CD10, and ROR1) has been defined and will be prospectively evaluated. © 2017 International Clinical Cytometry Society John Wiley and Sons Inc. 2018-01-17 2018-01 /pmc/articles/PMC5817234/ /pubmed/29024461 http://dx.doi.org/10.1002/cyto.b.21595 Text en © 2017 The Authors. Cytometry Part B: Clinical Cytometry published by Wiley Periodicals, Inc. on behalf of International Clinical Cytometry Society This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial (http://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
spellingShingle | Original Articles Rawstron, Andy C. Kreuzer, Karl‐Anton Soosapilla, Asha Spacek, Martin Stehlikova, Olga Gambell, Peter McIver‐Brown, Neil Villamor, Neus Psarra, Katherina Arroz, Maria Milani, Raffaella de la Serna, Javier Cedena, M. Teresa Jaksic, Ozren Nomdedeu, Josep Moreno, Carol Rigolin, Gian Matteo Cuneo, Antonio Johansen, Preben Johnsen, Hans E. Rosenquist, Richard Niemann, Carsten Utoft Kern, Wolfgang Westerman, David Trneny, Marek Mulligan, Stephen Doubek, Michael Pospisilova, Sarka Hillmen, Peter Oscier, David Hallek, Michael Ghia, Paolo Montserrat, Emili Reproducible diagnosis of chronic lymphocytic leukemia by flow cytometry: An European Research Initiative on CLL (ERIC) & European Society for Clinical Cell Analysis (ESCCA) Harmonisation project |
title | Reproducible diagnosis of chronic lymphocytic leukemia by flow cytometry: An European Research Initiative on CLL (ERIC) & European Society for Clinical Cell Analysis (ESCCA) Harmonisation project |
title_full | Reproducible diagnosis of chronic lymphocytic leukemia by flow cytometry: An European Research Initiative on CLL (ERIC) & European Society for Clinical Cell Analysis (ESCCA) Harmonisation project |
title_fullStr | Reproducible diagnosis of chronic lymphocytic leukemia by flow cytometry: An European Research Initiative on CLL (ERIC) & European Society for Clinical Cell Analysis (ESCCA) Harmonisation project |
title_full_unstemmed | Reproducible diagnosis of chronic lymphocytic leukemia by flow cytometry: An European Research Initiative on CLL (ERIC) & European Society for Clinical Cell Analysis (ESCCA) Harmonisation project |
title_short | Reproducible diagnosis of chronic lymphocytic leukemia by flow cytometry: An European Research Initiative on CLL (ERIC) & European Society for Clinical Cell Analysis (ESCCA) Harmonisation project |
title_sort | reproducible diagnosis of chronic lymphocytic leukemia by flow cytometry: an european research initiative on cll (eric) & european society for clinical cell analysis (escca) harmonisation project |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5817234/ https://www.ncbi.nlm.nih.gov/pubmed/29024461 http://dx.doi.org/10.1002/cyto.b.21595 |
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