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Differential Effects of Three Techniques for Hepatic Vascular Exclusion during Resection for Liver Cirrhosis on Hepatic Ischemia-Reperfusion Injury in Rats

BACKGROUND/AIMS: Hepatic ischemia-reperfusion (I/R) injury is a serious concern during hepatic vascular occlusion. The objectives of this study were to assess effects of three techniques for hepatic vascular occlusion on I/R injury and to explore the underlying mechanisms. METHODS: Liver cirrhotic r...

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Autores principales: Jia, Changjun, Dai, Chaoliu, Wang, Hailiang, Wan, Yi, Qiao, Yunyu, Xu, Feng, Peng, Songlin, Zhao, Yang, Zhao, Chuang, Zhao, Liang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5817263/
https://www.ncbi.nlm.nih.gov/pubmed/29507582
http://dx.doi.org/10.1155/2018/5309286
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author Jia, Changjun
Dai, Chaoliu
Wang, Hailiang
Wan, Yi
Qiao, Yunyu
Xu, Feng
Peng, Songlin
Zhao, Yang
Zhao, Chuang
Zhao, Liang
author_facet Jia, Changjun
Dai, Chaoliu
Wang, Hailiang
Wan, Yi
Qiao, Yunyu
Xu, Feng
Peng, Songlin
Zhao, Yang
Zhao, Chuang
Zhao, Liang
author_sort Jia, Changjun
collection PubMed
description BACKGROUND/AIMS: Hepatic ischemia-reperfusion (I/R) injury is a serious concern during hepatic vascular occlusion. The objectives of this study were to assess effects of three techniques for hepatic vascular occlusion on I/R injury and to explore the underlying mechanisms. METHODS: Liver cirrhotic rats had undertaken Pringle maneuver (PR), hemihepatic vascular occlusion (HH), or hepatic blood inflow occlusion without hemihepatic artery control (WH). Levels of tumor necrosis factor alpha (TNF-α), nuclear factor kappa B (NF-κB), toll-like receptor 4 (TLR4), TIR-domain-containing adapter-inducing interferon-β (TRIF), and hemeoxygenase 1 (HMOX1) were assayed. RESULTS: The histopathologic analysis displayed that liver harm was more prominent in the PR group, but similar in the HH and WH groups. The HH and WH groups responded to hepatic I/R inflammation similarly but better than the PR group. Mechanical studies suggested that TNF-α/NF-κB signaling and TLR4/TRIF transduction pathways were associated with the differential effects. In addition, the HH and WH groups had significantly higher levels of hepatic HMOX1 (P < 0.05) than the PR group. CONCLUSIONS: HH and WH confer better preservation of liver function and protection than the Pringle maneuver in combating I/R injury. Upregulation of HMOX1 may lead to better protection and clinical outcomes after liver resection.
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spelling pubmed-58172632018-03-05 Differential Effects of Three Techniques for Hepatic Vascular Exclusion during Resection for Liver Cirrhosis on Hepatic Ischemia-Reperfusion Injury in Rats Jia, Changjun Dai, Chaoliu Wang, Hailiang Wan, Yi Qiao, Yunyu Xu, Feng Peng, Songlin Zhao, Yang Zhao, Chuang Zhao, Liang Gastroenterol Res Pract Research Article BACKGROUND/AIMS: Hepatic ischemia-reperfusion (I/R) injury is a serious concern during hepatic vascular occlusion. The objectives of this study were to assess effects of three techniques for hepatic vascular occlusion on I/R injury and to explore the underlying mechanisms. METHODS: Liver cirrhotic rats had undertaken Pringle maneuver (PR), hemihepatic vascular occlusion (HH), or hepatic blood inflow occlusion without hemihepatic artery control (WH). Levels of tumor necrosis factor alpha (TNF-α), nuclear factor kappa B (NF-κB), toll-like receptor 4 (TLR4), TIR-domain-containing adapter-inducing interferon-β (TRIF), and hemeoxygenase 1 (HMOX1) were assayed. RESULTS: The histopathologic analysis displayed that liver harm was more prominent in the PR group, but similar in the HH and WH groups. The HH and WH groups responded to hepatic I/R inflammation similarly but better than the PR group. Mechanical studies suggested that TNF-α/NF-κB signaling and TLR4/TRIF transduction pathways were associated with the differential effects. In addition, the HH and WH groups had significantly higher levels of hepatic HMOX1 (P < 0.05) than the PR group. CONCLUSIONS: HH and WH confer better preservation of liver function and protection than the Pringle maneuver in combating I/R injury. Upregulation of HMOX1 may lead to better protection and clinical outcomes after liver resection. Hindawi 2018-01-08 /pmc/articles/PMC5817263/ /pubmed/29507582 http://dx.doi.org/10.1155/2018/5309286 Text en Copyright © 2018 Changjun Jia et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Jia, Changjun
Dai, Chaoliu
Wang, Hailiang
Wan, Yi
Qiao, Yunyu
Xu, Feng
Peng, Songlin
Zhao, Yang
Zhao, Chuang
Zhao, Liang
Differential Effects of Three Techniques for Hepatic Vascular Exclusion during Resection for Liver Cirrhosis on Hepatic Ischemia-Reperfusion Injury in Rats
title Differential Effects of Three Techniques for Hepatic Vascular Exclusion during Resection for Liver Cirrhosis on Hepatic Ischemia-Reperfusion Injury in Rats
title_full Differential Effects of Three Techniques for Hepatic Vascular Exclusion during Resection for Liver Cirrhosis on Hepatic Ischemia-Reperfusion Injury in Rats
title_fullStr Differential Effects of Three Techniques for Hepatic Vascular Exclusion during Resection for Liver Cirrhosis on Hepatic Ischemia-Reperfusion Injury in Rats
title_full_unstemmed Differential Effects of Three Techniques for Hepatic Vascular Exclusion during Resection for Liver Cirrhosis on Hepatic Ischemia-Reperfusion Injury in Rats
title_short Differential Effects of Three Techniques for Hepatic Vascular Exclusion during Resection for Liver Cirrhosis on Hepatic Ischemia-Reperfusion Injury in Rats
title_sort differential effects of three techniques for hepatic vascular exclusion during resection for liver cirrhosis on hepatic ischemia-reperfusion injury in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5817263/
https://www.ncbi.nlm.nih.gov/pubmed/29507582
http://dx.doi.org/10.1155/2018/5309286
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