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Country-Wide Surveillance of Molecular Markers of Antimalarial Drug Resistance in Senegal by Use of Positive Malaria Rapid Diagnostic Tests

In Senegal, antimalarial drugs used in treatment and prevention of malaria are one of the main reasons for the current success in controlling malaria. However, the successful control of malaria is highly dependent on continued effectiveness of these drugs which may be compromised by the spread of dr...

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Autores principales: Ndiaye, Magatte, Sow, Doudou, Nag, Sidsel, Sylla, Khadime, Tine, Roger Clement, Ndiaye, Jean Louis, Lo, Aminata Collé, Gaye, Oumar, Faye, Babacar, Alifrangis, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society of Tropical Medicine and Hygiene 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5817740/
https://www.ncbi.nlm.nih.gov/pubmed/29140232
http://dx.doi.org/10.4269/ajtmh.17-0021
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author Ndiaye, Magatte
Sow, Doudou
Nag, Sidsel
Sylla, Khadime
Tine, Roger Clement
Ndiaye, Jean Louis
Lo, Aminata Collé
Gaye, Oumar
Faye, Babacar
Alifrangis, Michael
author_facet Ndiaye, Magatte
Sow, Doudou
Nag, Sidsel
Sylla, Khadime
Tine, Roger Clement
Ndiaye, Jean Louis
Lo, Aminata Collé
Gaye, Oumar
Faye, Babacar
Alifrangis, Michael
author_sort Ndiaye, Magatte
collection PubMed
description In Senegal, antimalarial drugs used in treatment and prevention of malaria are one of the main reasons for the current success in controlling malaria. However, the successful control of malaria is highly dependent on continued effectiveness of these drugs which may be compromised by the spread of drug resistance. Therefore, surveillance of drug resistance in the malaria parasites is essential. The objective of this pilot study was to test the feasibility of routinely sampled malaria rapid diagnostic tests (RDTs) at a national scale to assess the temporal changes in the molecular profiles of antimalarial drug resistance markers of Plasmodium falciparum parasites. Overall, 9,549 positive malaria RDTs were collected from 14 health facilities across the country. A limited random set of RDTs were analyzed regarding Pfcrt gene polymorphisms at codon 72–76. Overall, a high but varied prevalence (> 50%) of the wild-type CVMNK haplotype was observed including a higher CVMNK prevalence in the northern part (75%) compared with the southern part of the country (59%). With caution, the study provides a proof of concept that reuse of discarded P. falciparum positive RDTs can be applied in large-scale surveillance of antimalarial drug resistance.
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spelling pubmed-58177402018-04-30 Country-Wide Surveillance of Molecular Markers of Antimalarial Drug Resistance in Senegal by Use of Positive Malaria Rapid Diagnostic Tests Ndiaye, Magatte Sow, Doudou Nag, Sidsel Sylla, Khadime Tine, Roger Clement Ndiaye, Jean Louis Lo, Aminata Collé Gaye, Oumar Faye, Babacar Alifrangis, Michael Am J Trop Med Hyg Articles In Senegal, antimalarial drugs used in treatment and prevention of malaria are one of the main reasons for the current success in controlling malaria. However, the successful control of malaria is highly dependent on continued effectiveness of these drugs which may be compromised by the spread of drug resistance. Therefore, surveillance of drug resistance in the malaria parasites is essential. The objective of this pilot study was to test the feasibility of routinely sampled malaria rapid diagnostic tests (RDTs) at a national scale to assess the temporal changes in the molecular profiles of antimalarial drug resistance markers of Plasmodium falciparum parasites. Overall, 9,549 positive malaria RDTs were collected from 14 health facilities across the country. A limited random set of RDTs were analyzed regarding Pfcrt gene polymorphisms at codon 72–76. Overall, a high but varied prevalence (> 50%) of the wild-type CVMNK haplotype was observed including a higher CVMNK prevalence in the northern part (75%) compared with the southern part of the country (59%). With caution, the study provides a proof of concept that reuse of discarded P. falciparum positive RDTs can be applied in large-scale surveillance of antimalarial drug resistance. The American Society of Tropical Medicine and Hygiene 2017-11-08 2017-08-28 /pmc/articles/PMC5817740/ /pubmed/29140232 http://dx.doi.org/10.4269/ajtmh.17-0021 Text en © The American Society of Tropical Medicine and Hygiene This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Articles
Ndiaye, Magatte
Sow, Doudou
Nag, Sidsel
Sylla, Khadime
Tine, Roger Clement
Ndiaye, Jean Louis
Lo, Aminata Collé
Gaye, Oumar
Faye, Babacar
Alifrangis, Michael
Country-Wide Surveillance of Molecular Markers of Antimalarial Drug Resistance in Senegal by Use of Positive Malaria Rapid Diagnostic Tests
title Country-Wide Surveillance of Molecular Markers of Antimalarial Drug Resistance in Senegal by Use of Positive Malaria Rapid Diagnostic Tests
title_full Country-Wide Surveillance of Molecular Markers of Antimalarial Drug Resistance in Senegal by Use of Positive Malaria Rapid Diagnostic Tests
title_fullStr Country-Wide Surveillance of Molecular Markers of Antimalarial Drug Resistance in Senegal by Use of Positive Malaria Rapid Diagnostic Tests
title_full_unstemmed Country-Wide Surveillance of Molecular Markers of Antimalarial Drug Resistance in Senegal by Use of Positive Malaria Rapid Diagnostic Tests
title_short Country-Wide Surveillance of Molecular Markers of Antimalarial Drug Resistance in Senegal by Use of Positive Malaria Rapid Diagnostic Tests
title_sort country-wide surveillance of molecular markers of antimalarial drug resistance in senegal by use of positive malaria rapid diagnostic tests
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5817740/
https://www.ncbi.nlm.nih.gov/pubmed/29140232
http://dx.doi.org/10.4269/ajtmh.17-0021
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