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Variant cell cycles regulated by Notch signaling control cell size and ensure a functional blood-brain barrier
Regulation of cell size is crucial in development. In plants and animals two cell cycle variants are employed to generate large cells by increased ploidy: the endocycle and endomitosis. The rationale behind the choice of which of these cycles is implemented is unknown. We show that in the Drosophila...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists Ltd
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5818001/ https://www.ncbi.nlm.nih.gov/pubmed/29440220 http://dx.doi.org/10.1242/dev.157115 |
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author | Von Stetina, Jessica R. Frawley, Laura E. Unhavaithaya, Yingdee Orr-Weaver, Terry L. |
author_facet | Von Stetina, Jessica R. Frawley, Laura E. Unhavaithaya, Yingdee Orr-Weaver, Terry L. |
author_sort | Von Stetina, Jessica R. |
collection | PubMed |
description | Regulation of cell size is crucial in development. In plants and animals two cell cycle variants are employed to generate large cells by increased ploidy: the endocycle and endomitosis. The rationale behind the choice of which of these cycles is implemented is unknown. We show that in the Drosophila nervous system the subperineurial glia (SPG) are unique in using both the endocycle and endomitosis to grow. In the brain, the majority of SPG initially endocycle, then switch to endomitosis during larval development. The Notch signaling pathway and the String Cdc25 phosphatase are crucial for the endocycle versus endomitosis choice, providing the means experimentally to change cells from one to the other. This revealed fundamental insights into the control of cell size and the properties of endomitotic cells. Endomitotic cells attain a higher ploidy and larger size than endocycling cells, and endomitotic SPG are necessary for the blood-brain barrier. Decreased Notch signaling promotes endomitosis even in the ventral nerve cord SPG that normally are mononucleate, but not in the endocycling salivary gland cells, revealing tissue-specific cell cycle responses. |
format | Online Article Text |
id | pubmed-5818001 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | The Company of Biologists Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-58180012018-02-28 Variant cell cycles regulated by Notch signaling control cell size and ensure a functional blood-brain barrier Von Stetina, Jessica R. Frawley, Laura E. Unhavaithaya, Yingdee Orr-Weaver, Terry L. Development Research Article Regulation of cell size is crucial in development. In plants and animals two cell cycle variants are employed to generate large cells by increased ploidy: the endocycle and endomitosis. The rationale behind the choice of which of these cycles is implemented is unknown. We show that in the Drosophila nervous system the subperineurial glia (SPG) are unique in using both the endocycle and endomitosis to grow. In the brain, the majority of SPG initially endocycle, then switch to endomitosis during larval development. The Notch signaling pathway and the String Cdc25 phosphatase are crucial for the endocycle versus endomitosis choice, providing the means experimentally to change cells from one to the other. This revealed fundamental insights into the control of cell size and the properties of endomitotic cells. Endomitotic cells attain a higher ploidy and larger size than endocycling cells, and endomitotic SPG are necessary for the blood-brain barrier. Decreased Notch signaling promotes endomitosis even in the ventral nerve cord SPG that normally are mononucleate, but not in the endocycling salivary gland cells, revealing tissue-specific cell cycle responses. The Company of Biologists Ltd 2018-02-01 /pmc/articles/PMC5818001/ /pubmed/29440220 http://dx.doi.org/10.1242/dev.157115 Text en © 2018. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Research Article Von Stetina, Jessica R. Frawley, Laura E. Unhavaithaya, Yingdee Orr-Weaver, Terry L. Variant cell cycles regulated by Notch signaling control cell size and ensure a functional blood-brain barrier |
title | Variant cell cycles regulated by Notch signaling control cell size and ensure a functional blood-brain barrier |
title_full | Variant cell cycles regulated by Notch signaling control cell size and ensure a functional blood-brain barrier |
title_fullStr | Variant cell cycles regulated by Notch signaling control cell size and ensure a functional blood-brain barrier |
title_full_unstemmed | Variant cell cycles regulated by Notch signaling control cell size and ensure a functional blood-brain barrier |
title_short | Variant cell cycles regulated by Notch signaling control cell size and ensure a functional blood-brain barrier |
title_sort | variant cell cycles regulated by notch signaling control cell size and ensure a functional blood-brain barrier |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5818001/ https://www.ncbi.nlm.nih.gov/pubmed/29440220 http://dx.doi.org/10.1242/dev.157115 |
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