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Circulatory white spot syndrome virus in South-West region of Bangladesh from 2014 to 2017: molecular characterization and genetic variation
White Spot Syndrome Virus (WSSV), the etiological agent of White Spot Disease (WSD) is a major impediment for shrimp aquaculture in the worldwide. A critical threshold level of WSSV load in infected shrimp is an important trait for disease manifestation and WSSV transmission in cultured shrimp and s...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5818386/ https://www.ncbi.nlm.nih.gov/pubmed/29460184 http://dx.doi.org/10.1186/s13568-018-0553-z |
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author | Siddique, Mohammad Anwar Haque, Md. Inja-Mamun Sanyal, Santonu Kumar Hossain, Anwar Nandi, Shuvro Prokash Alam, A. S. M. Rubayet Ul Sultana, Munawar Hasan, Mahmud Hossain, M. Anwar |
author_facet | Siddique, Mohammad Anwar Haque, Md. Inja-Mamun Sanyal, Santonu Kumar Hossain, Anwar Nandi, Shuvro Prokash Alam, A. S. M. Rubayet Ul Sultana, Munawar Hasan, Mahmud Hossain, M. Anwar |
author_sort | Siddique, Mohammad Anwar |
collection | PubMed |
description | White Spot Syndrome Virus (WSSV), the etiological agent of White Spot Disease (WSD) is a major impediment for shrimp aquaculture in the worldwide. A critical threshold level of WSSV load in infected shrimp is an important trait for disease manifestation and WSSV transmission in cultured shrimp and subsequently make outbreaks. The present study investigated 120 naturally infected cultured shrimp samples by SYBR Green based qPCR assay for WSD diagnosis and quantification of WSSV load. Among them, 94 samples resulted a variable count of WSSV load ranging from 2.1 × 10(8) to 2.64 × 10(14) copies/g of shrimp tissue. The severity of WSSV infection was assessed based on the established critical threshold load of WSSV in shrimp tissue. Compared to the established critical threshold value of WSSV load in shrimp tissue, our findings showed the horrifying scenario of the severity of WSSV infection in cultured shrimps of Bangladesh that was found to be above the critical limit to initiate an outbreak in the Bangladeshi shrimp aquaculture industry. The latest phylogenetic pattern was altered from the former monophyletic history among WSSVs of Bangladesh due to a variation at 500th nucleotide of VP28 coding gene. Viruses characterized from recent outbreaks in 2015 and 2017 displayed amino acid substitution at position 167 (G→E) on the surface of VP28 protein which has demonstrated the probable replacement of indigenous virus pool. Therefore, it is imperative to take initiative for the management and prevention of WSSV outbreak to sustain shrimp aquaculture in South-West region of Bangladesh. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13568-018-0553-z) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5818386 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-58183862018-02-27 Circulatory white spot syndrome virus in South-West region of Bangladesh from 2014 to 2017: molecular characterization and genetic variation Siddique, Mohammad Anwar Haque, Md. Inja-Mamun Sanyal, Santonu Kumar Hossain, Anwar Nandi, Shuvro Prokash Alam, A. S. M. Rubayet Ul Sultana, Munawar Hasan, Mahmud Hossain, M. Anwar AMB Express Original Article White Spot Syndrome Virus (WSSV), the etiological agent of White Spot Disease (WSD) is a major impediment for shrimp aquaculture in the worldwide. A critical threshold level of WSSV load in infected shrimp is an important trait for disease manifestation and WSSV transmission in cultured shrimp and subsequently make outbreaks. The present study investigated 120 naturally infected cultured shrimp samples by SYBR Green based qPCR assay for WSD diagnosis and quantification of WSSV load. Among them, 94 samples resulted a variable count of WSSV load ranging from 2.1 × 10(8) to 2.64 × 10(14) copies/g of shrimp tissue. The severity of WSSV infection was assessed based on the established critical threshold load of WSSV in shrimp tissue. Compared to the established critical threshold value of WSSV load in shrimp tissue, our findings showed the horrifying scenario of the severity of WSSV infection in cultured shrimps of Bangladesh that was found to be above the critical limit to initiate an outbreak in the Bangladeshi shrimp aquaculture industry. The latest phylogenetic pattern was altered from the former monophyletic history among WSSVs of Bangladesh due to a variation at 500th nucleotide of VP28 coding gene. Viruses characterized from recent outbreaks in 2015 and 2017 displayed amino acid substitution at position 167 (G→E) on the surface of VP28 protein which has demonstrated the probable replacement of indigenous virus pool. Therefore, it is imperative to take initiative for the management and prevention of WSSV outbreak to sustain shrimp aquaculture in South-West region of Bangladesh. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13568-018-0553-z) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2018-02-20 /pmc/articles/PMC5818386/ /pubmed/29460184 http://dx.doi.org/10.1186/s13568-018-0553-z Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Article Siddique, Mohammad Anwar Haque, Md. Inja-Mamun Sanyal, Santonu Kumar Hossain, Anwar Nandi, Shuvro Prokash Alam, A. S. M. Rubayet Ul Sultana, Munawar Hasan, Mahmud Hossain, M. Anwar Circulatory white spot syndrome virus in South-West region of Bangladesh from 2014 to 2017: molecular characterization and genetic variation |
title | Circulatory white spot syndrome virus in South-West region of Bangladesh from 2014 to 2017: molecular characterization and genetic variation |
title_full | Circulatory white spot syndrome virus in South-West region of Bangladesh from 2014 to 2017: molecular characterization and genetic variation |
title_fullStr | Circulatory white spot syndrome virus in South-West region of Bangladesh from 2014 to 2017: molecular characterization and genetic variation |
title_full_unstemmed | Circulatory white spot syndrome virus in South-West region of Bangladesh from 2014 to 2017: molecular characterization and genetic variation |
title_short | Circulatory white spot syndrome virus in South-West region of Bangladesh from 2014 to 2017: molecular characterization and genetic variation |
title_sort | circulatory white spot syndrome virus in south-west region of bangladesh from 2014 to 2017: molecular characterization and genetic variation |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5818386/ https://www.ncbi.nlm.nih.gov/pubmed/29460184 http://dx.doi.org/10.1186/s13568-018-0553-z |
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