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Human antibody responses against non-covalently cell wall-bound Staphylococcus aureus proteins

Human antibody responses to pathogens, like Staphylococcus aureus, are important indicators for in vivo expression and immunogenicity of particular bacterial components. Accordingly, comparing the antibody responses to S. aureus components may serve to predict their potential applicability as antige...

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Autores principales: Romero Pastrana, Francisco, Neef, Jolanda, Koedijk, Dennis G. A. M., de Graaf, Douwe, Duipmans, José, Jonkman, Marcel F., Engelmann, Susanne, van Dijl, Jan Maarten, Buist, Girbe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5818649/
https://www.ncbi.nlm.nih.gov/pubmed/29459694
http://dx.doi.org/10.1038/s41598-018-21724-z
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author Romero Pastrana, Francisco
Neef, Jolanda
Koedijk, Dennis G. A. M.
de Graaf, Douwe
Duipmans, José
Jonkman, Marcel F.
Engelmann, Susanne
van Dijl, Jan Maarten
Buist, Girbe
author_facet Romero Pastrana, Francisco
Neef, Jolanda
Koedijk, Dennis G. A. M.
de Graaf, Douwe
Duipmans, José
Jonkman, Marcel F.
Engelmann, Susanne
van Dijl, Jan Maarten
Buist, Girbe
author_sort Romero Pastrana, Francisco
collection PubMed
description Human antibody responses to pathogens, like Staphylococcus aureus, are important indicators for in vivo expression and immunogenicity of particular bacterial components. Accordingly, comparing the antibody responses to S. aureus components may serve to predict their potential applicability as antigens for vaccination. The present study was aimed at assessing immunoglobulin G (IgG) responses elicited by non-covalently cell surface-bound proteins of S. aureus, which thus far received relatively little attention. To this end, we applied plasma samples from patients with the genetic blistering disease epidermolysis bullosa (EB) and healthy S. aureus carriers. Of note, wounds of EB patients are highly colonized with S. aureus and accordingly these patients are more seriously exposed to staphylococcal antigens than healthy individuals. Ten non-covalently cell surface-bound proteins of S. aureus, namely Atl, Eap, Efb, EMP, IsaA, LukG, LukH, SA0710, Sle1 and SsaA2, were selected by bioinformatics and biochemical approaches. These antigens were recombinantly expressed, purified and tested for specific IgG responses using human plasma. We show that high exposure of EB patients to S. aureus is mirrored by elevated IgG levels against all tested non-covalently cell wall-bound staphylococcal antigens. This implies that these S. aureus cell surface proteins are prime targets for the human immune system.
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spelling pubmed-58186492018-02-26 Human antibody responses against non-covalently cell wall-bound Staphylococcus aureus proteins Romero Pastrana, Francisco Neef, Jolanda Koedijk, Dennis G. A. M. de Graaf, Douwe Duipmans, José Jonkman, Marcel F. Engelmann, Susanne van Dijl, Jan Maarten Buist, Girbe Sci Rep Article Human antibody responses to pathogens, like Staphylococcus aureus, are important indicators for in vivo expression and immunogenicity of particular bacterial components. Accordingly, comparing the antibody responses to S. aureus components may serve to predict their potential applicability as antigens for vaccination. The present study was aimed at assessing immunoglobulin G (IgG) responses elicited by non-covalently cell surface-bound proteins of S. aureus, which thus far received relatively little attention. To this end, we applied plasma samples from patients with the genetic blistering disease epidermolysis bullosa (EB) and healthy S. aureus carriers. Of note, wounds of EB patients are highly colonized with S. aureus and accordingly these patients are more seriously exposed to staphylococcal antigens than healthy individuals. Ten non-covalently cell surface-bound proteins of S. aureus, namely Atl, Eap, Efb, EMP, IsaA, LukG, LukH, SA0710, Sle1 and SsaA2, were selected by bioinformatics and biochemical approaches. These antigens were recombinantly expressed, purified and tested for specific IgG responses using human plasma. We show that high exposure of EB patients to S. aureus is mirrored by elevated IgG levels against all tested non-covalently cell wall-bound staphylococcal antigens. This implies that these S. aureus cell surface proteins are prime targets for the human immune system. Nature Publishing Group UK 2018-02-19 /pmc/articles/PMC5818649/ /pubmed/29459694 http://dx.doi.org/10.1038/s41598-018-21724-z Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Romero Pastrana, Francisco
Neef, Jolanda
Koedijk, Dennis G. A. M.
de Graaf, Douwe
Duipmans, José
Jonkman, Marcel F.
Engelmann, Susanne
van Dijl, Jan Maarten
Buist, Girbe
Human antibody responses against non-covalently cell wall-bound Staphylococcus aureus proteins
title Human antibody responses against non-covalently cell wall-bound Staphylococcus aureus proteins
title_full Human antibody responses against non-covalently cell wall-bound Staphylococcus aureus proteins
title_fullStr Human antibody responses against non-covalently cell wall-bound Staphylococcus aureus proteins
title_full_unstemmed Human antibody responses against non-covalently cell wall-bound Staphylococcus aureus proteins
title_short Human antibody responses against non-covalently cell wall-bound Staphylococcus aureus proteins
title_sort human antibody responses against non-covalently cell wall-bound staphylococcus aureus proteins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5818649/
https://www.ncbi.nlm.nih.gov/pubmed/29459694
http://dx.doi.org/10.1038/s41598-018-21724-z
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